Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 246-186-9 | CAS number: 24347-58-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 15 June 1999 -17 June 1999
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- guideline version from 1984
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 1984
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Batch no. 34-2127
Date of production: December 1998
Colour: colourless/yellowish - Analytical monitoring:
- yes
- Details on sampling:
- Sample preparation: To the whole sample (5 ml in a 10 ml vial) 4.5 ml drinking water and 0.5 ml IS solution were added.
The concentration control analyses were performed with test solutions of the following nominal concentrations (mg/l) of the test substance: 0 (control), 100, 12.5
At the start of the test (after 0 h) samples from vessels without daphnids, at the end of the test (after 48 h) samples from vessels with and without daphnids were analyzed. - Vehicle:
- no
- Details on test solutions:
- - Preparation of the stock solution and dilution
The test substance was stirred in M4 medium for about 20 hours at 20 ± 2°C. Undissolved test substance was removed by centrifugation (approximately 20 min at about 17700 G). The nominal concentration of the eluate was 100 mg/I.
- The test substance was tested in the range of concentrations between 12,5 and 100 mg/I. The dilution factor was 2. The test was performed with an aqueous extract (eluate) of the test substance (dilution with with M4 medium). - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Strain: STRAUS
- Source: The clone of Daphnia magna STRAUS 1820 used was supplied by the Institut National de Recherche Chimique Appliquée, France, in 1978. From this date on this clone was cultured and bred continuously in the Laboratory of Ecology of BASF AG in Ludwigshafen.
- Age at study initiation (mean and range, SD): 2 - 24 h ( starting with the 3rd breed of parent animals) - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 2.20 - 3.20 mmol/l
- Test temperature:
- 19.9-20.3
- pH:
- 7.9-8.0
- Dissolved oxygen:
- 8.0-8.7 mg/L
- Conductivity:
- 550 - 650 µS/cm
- Nominal and measured concentrations:
- Nominal concentrations: 0 (control), 100, 20, 25, 12.5 mg/L
Measured concentrations: < 5, 13.2, 104 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: Test tubes (glass) with flat bottom (nominal volume 20 ml)
- Type (delete if not applicable): open
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Number of parallels without animals:
1 (parallel 5) per test concentration and control for measurement of the pH and oxygen content at the beginning of the test. This parallel was thrown away after measurement.
2 per test concentration and control for concentration control analysis.
OTHER TEST CONDITIONS
- Photoperiod: Artificial light, type warm white (e.g: OSRAM L58 W31) day:night rhythm = 16 : 8 h
- Light intensity: About 2 - 7 NE/(m^2•s) at a wave length of 400 - 700 nm
- Measurement of temperature: Continuously during the whole test period in a separate vessel close to the test vessels.
- Measurement of pH and oxygen: After Oh in an additional replicate without daphnids and after 48h in replicate 1 of each tested concentration.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Test parameter: Swimming ability of the test animals; visually after 0, 24 and 48 h
RANGE-FINDING STUDY
- No - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: Nominal concentration verified analytically
- Key result
- Duration:
- 48 d
- Dose descriptor:
- EC50
- Effect conc.:
- 147 mg/L
- Nominal / measured:
- estimated
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: extrapolated value
- Remarks:
- 95% CI: 112 - 240
- Details on results:
- No immobility of daphnids was observed at test concentrations 12.5 and 20 mg/L during exposure period of 24 hours and 48 hours. One daphnid was observed immobile at 50 mg/L after 48 hours of exposure, and two daphnids were observed immobile at 100 mg/L after 48 hours.
Based on a new dose-response assessment of the available data with the online modelling tool MOSAIC Surv-Standard (MOdeling and StAtistical tools for ecotoxICology), an 48h-EC50 value was estimated (extrapolated) at 147 mg/L (95% CI: 112 - 240).
No immobility was observed in the control. - Results with reference substance (positive control):
- The EC50(24h) of the control substance potassium dichromate was 1.30 mg/I.
(Date of the last control experiment: 25 May 1999). - Reported statistics and error estimates:
- No statistical evaluation were performed in the original study, because adverse effects were <= 10 % immobilisation at 100 mg/L.
A new curve-fitting assessment was performed with the online modelling tool MOSAIC Surv-Standard (MOdeling and StAtistical tools for ecotoxICology), Laboratoire de Biométrie et Biologie Évolutive Université de Lyon. - Validity criteria fulfilled:
- yes
- Remarks:
- according to OECD adopted 1984, 2004
- Conclusions:
- The derived effect concentration 48h-EC50 (Daphnia magna, immobilisation) > 100 mg/L (nominal); 48-EC50 value was estimated (extrapolated) at 147 mg/L (95% CI: 112 - 240).
- Executive summary:
The study investigated acute toxicity of 2,3 -Butanediol to aquatic invertebrate Daphnia magna. The study was performed according to OECD guideline 202 (adopted 1984) and in compliance with GLP. The study was performed under static test conditions at the following test concentrations: 0 (control), 12.5, 25, 50 and 100 mg/L. Test concentrations 0 (control), 12.5 and 100 mg/L were analytically verified.
No immobility of daphnids was observed at test concentrations 0 (control), 12.5 and 20 mg/L during exposure period of 24 hours and 48 hours. One daphnid was observed immobile at 50 mg/L after 48 hours of exposure, and two daphnids were observed immobile at 100 mg/L after 48 hours. As this is considered within the acceptable number of immobile daphnids within a valid control according to the guideline, no further assessment of adverse effects were performed for this test within the study. The derived effect concentration is: 48h-EC50 > 100 mg/L was derived.
However, as there is a slight indication of a dose-response effect of immobility with increasing concentrations, a posterior curve fitting was performed with available data using the online modelling tool MOSAIC Surv-Standard (MOdeling and StAtistical tools for ecotoxICology). Based on the new dose-response assessment of MOSAIC Surv-Standard, the 48h-EC50 value was estimated (extrapolated) at 147 mg/L (95% CI: 112 - 240).
The validity criteria of the test guideline (adopted 1984) were fulfilled. Despite of some deficiencies with respect to the updated guideline, the study was considered adequate and reliable for environmental hazard assessment.
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- For details on endpoint specific justification please refer to the read-across report, endpoint specific justification.
- Reason / purpose for cross-reference:
- assessment report
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: Effect conc. are identical for the submission substance, because target and source substance have the same MW.
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 147 mg/L
- Nominal / measured:
- estimated
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: extrapolated value
- Remarks:
- Effect conc. are identical for the submission substance, because target and source substance have the same MW.
- Validity criteria fulfilled:
- yes
- Remarks:
- according to OECD adopted 1984, 2004
- Conclusions:
- The derived effect concentration 48h-EC50 (Daphnia magna, immobilisation) > 100 mg/L (nominal); 48-EC50 value was estimated (extrapolated) at 147 mg/L (95% CI: 112 - 240).
- Executive summary:
The study used as source investigated acute toxicity (immobility) to aquatic invertebrate Daphnia magna during 48 hours. The study results of the source compound were considered applicable to the target compound, and were used for classification and labelling acc. to Regulation (EC) No 1272/2008. Justification and applicability of the read-across approach (structural analogue) is outlined in the read-across report in section 13.
Referenceopen allclose all
Number of mobile Daphnids in the different test concentrations.
Conc.[mg/l] | Parallel 1 [h] |
Parallel 2 [h] |
Parallel 3 [h] |
Parallel 4 [h] |
∑ [h] |
|||||||||
0 | 24 | 48 | 0 | 24 | 48 | 0 | 24 | 48 | 0 | 24 | 48 | 24 | 48 | |
control | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 20 | 20 |
100 | 5 | 5 | 4 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 4 | 20 | 18 |
50 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 4 | 5 | 5 | 5 | 20 | 19 |
25 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 20 | 20 |
12,5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 20 | 20 |
Number of test animals per replicate: 5
Number of test animals per concentration: 20
Analytical measurements for test item concentrations
With an analytical recovery rate of > 80% no transformation of the nominal EC-values into the values of the concentration control analysis occurs.
Time [h] |
Stocked with daphnids | Minimum [%] |
Maximum [%] |
0 | no | 104,0 | 105,6 |
48 | no | 88,0 | 97,0 |
48 | yes | 89,6 | 97,0 |
Description of key information
The derived effect concentration 48h-EC50 (Daphnia magna, immobilisation) > 100 mg/L (nominal); 48-EC50 value was estimated (extrapolated) at 147 mg/L (95% CI: 112 - 240).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 147 mg/L
Additional information
No toxicity data concerning respiration inhibition of activated sludge is available for the submission substance. However, adequate and reliable data are reported here for a structural analogue (source substance 2,3 -butanediol). Please also refer to the read-across justification document.
In the available key study, acute toxicity to aquatic invertebrate Daphnia magna was investigated. The study was performed according to OECD guideline 202 (adopted 1984) and in compliance with GLP. No immobility of daphnids was observed at test concentrations 0 (control), 12.5 and 20 mg/L during exposure period of 24 hours and 48 hours. One daphnid was observed immobile at 50 mg/L after 48 hours of exposure, and two daphnids were observed immobile at 100 mg/L after 48 hours. As this is considered within the acceptable number of immobile daphnids within a valid control according to the guideline, no further assessment of adverse effects were performed for this test within the study. The derived effect concentration is: 48h-EC50 > 100 mg/L.
However, as there is a slight indication of a dose-response effect of immobility with increasing concentrations, a posterior curve fitting was performed with available data using the online modelling tool MOSAIC Surv-Standard (MOdeling and StAtistical tools for ecotoxICology). Based on the new dose-response assessment of MOSAIC Surv-Standard, the 48h-EC50 value was estimated (extrapolated) at 147 mg/L (95% CI: 112 - 240).
The validity criteria of the test guideline (adopted 1984) were fulfilled. Despite of some deficiencies with respect to the updated guideline, the study was considered adequate and reliable for environmental hazard assessment.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)