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EC number: 829-608-1 | CAS number: 106396-29-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 2017-11-08 to 2017-11-10
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 2004
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Purity: > 99%
Batch No.: 101Z4 - Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test sample 0.0500 g and dilution water 500 mL were mixed to prepare the nominal concentration of 100 mg/L, and they were stirred with a magnetic stirrer for 48 hours at about 20 °C. Total three suspensions were made in above same manner. Then, these suspension were filtered with a glass fiber filter by suction, and all filtrate were mixed. The mixed filtrate was stirred for 30 minutes at about 20 °C, then used as the stock solution.
- Controls: Dilution water processing similar to stock solution without the test item - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Strain/clone: Daphnia magna Clone A
- Source: The University of Sheffield, UK
- Age of parental stock (mean and range, SD): 14-day old
- Feeding during test: No feeding
ACCLIMATION
- Acclimation period:
- Acclimation conditions (same as test or not): same as test
- Type and amount of food: Chlorella vulgaris of 0.1-0.2 mgC (Organic carbon content)
- Feeding frequency: once a day
- Health during acclimation (any mortality observed): survival rate: 100% - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 32 mg/L (Ca, Mg)
- Test temperature:
- 20.0-20.2 °C
- pH:
- 7.5-7.8
- Dissolved oxygen:
- 7.3-8.8 mg/L
- Nominal and measured concentrations:
- Nominal: stock solution content of 100, 50.0, 25.0, 12.5 and 6.25%
Measured: 77.6, 38.4, 18.9, 9.17 and 4.57 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 mL glass beaker
- Aeration: No
- No. of organisms per vessel: 5 daphnia / test vessel
- No. of vessels per concentration (replicates): 4 replicates / test level
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Total organic carbon: < 0.5 mg/L
- Alkalinity: 36 mg/L
- Conductivity: 13 mS/m
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: Room light, 16-hour light / 8-hour dark
RANGE-FINDING STUDY
- Test concentrations: 5.00, 10.0, 50.0, 100% stock solution content and a control
- Results used to determine the conditions for the definitive study: The immobilization was 10%, 40% in 100% stock solution content at 24 and 48 hours, respectively. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 54.6 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% confìdence limits: 43.8-75.2 mg/L
- Details on results:
- - Other adverse effects control: one daphnid showed immobilization (5%). However, other daphnids did not show any abnormal response (discolor of body, trapping at the surface of the water and so on)
- Abnormal responses: Observed abnormal responses during exposure were immobilization, lethargic and reduced activity.
- Observation and measurement of test solution:
The test solution in the exposure level and the control were colorless and clear at the start and end of exposure.
The measured concentrations of the test item in the test solutions at the start of exposure were 4.48-78.1 mg/L, and those at the end of exposure were 4.66-77.1 mg/L which were 98.6% of the concentrations at the start of exposure.
- Immobility: The minimum concentration causing 100% immobility at 48 hours was >77.6 mg/L, because it was not obtained from the concentration range in the definitive study. Maximum concentration causing no immobility at 48 hours was 9.17 mg/L. - Results with reference substance (positive control):
- The 48-hour EC50 of a reference substance with the test organisms was 0.12 mg/L. This value was within the stipulated range (mean ± 2S.D.) [mean ± S.D.: 0 .23 ± 0.06 mg/L (n= 102)] to background data in this laboratory.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 24-hour and 48-hour EC50 of the test item for Daphnia magna were > 77.6 mg/L and 54.6 mg/L (95% confidence limits: 43.8-75.2 mg/L), respectively.
- Executive summary:
The objective of this study is to determine the 48-hour median effective concentration (EC50) by conducting an acute immobilization study of the test item in Daphnia magna according to OECD Guideline 202.
Five test concentrations of 100, 50.0, 25.0, 12.5, 6.25% as stock solution content (a geometric series with a factor of 2.0) (77.6, 38.4, 18.9, 9.17, 4.57 mg/L as measured concentrations) and a control were prepared. Daphnia were exposed to the test solutions of these concentrations for 48 h.
The minimum concentration causing 100% immobility at 48 hours was > 77.6 mg/L, because it was not obtained from the concentration range in the definitive study. Maximum concentration causing no immobility at 48 hours was 9.17 mg/L. Immobility in the control during exposure was 5%. Observed abnormal responses during exposure were immobilization, lethargic and reduced activity. In the control, one daphnid showed immobilization (5%). However, other daphnids did not show any abnormal response (discolor of body, trapping at the surface of the water and so on).
The measured concentrations of the test item in the test solutions at the start of exposure were 4.48-78.1 mg/L, and those at the end of exposure were 4.66-77.1 mg/L which were 98.6% of the concentrations at the start of exposure.
The 24-hour and 48-hour EC50 of the test item for Daphnia magna were >77.6 mg/L and 54.6 mg/L (95% confidence limits: 43.8-75.2 mg/L), respectively.
Reference
Description of key information
The 24-hour and 48-hour EC50 of the test item for Daphnia magna were > 77.6 mg/L and 54.6 mg/L (95% confidence limits: 43.8-75.2 mg/L), respectively.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 54.6 mg/L
Additional information
The objective of this study is to determine the 48-hour median effective concentration (EC50) by conducting an acute immobilization study of the test item in Daphnia magna according to OECD Guideline 202.
Five test concentrations of 100, 50.0, 25.0, 12.5, 6.25% as stock solution content (a geometric series with a factor of 2.0) (77.6, 38.4, 18.9, 9.17, 4.57 mg/L as measured concentrations) and a control were prepared. Daphnia were exposed to the test solutions of these concentrations for 48 h.
The minimum concentration causing 100% immobility at 48 hours was > 77.6 mg/L, because it was not obtained from the concentration range in the definitive study. Maximum concentration causing no immobility at 48 hours was 9.17 mg/L. Immobility in the control during exposure was 5%. Observed abnormal responses during exposure were immobilization, lethargic and reduced activity. In the control, one daphnid showed immobilization (5%). However, other daphnids did not show any abnormal response (discolor of body, trapping at the surface of the water and so on).
The measured concentrations of the test item in the test solutions at the start of exposure were 4.48-78.1 mg/L, and those at the end of exposure were 4.66-77.1 mg/L which were 98.6% of the concentrations at the start of exposure.
The 24-hour and 48-hour EC50 of the test item for Daphnia magna were >77.6 mg/L and 54.6 mg/L (95% confidence limits: 43.8-75.2 mg/L), respectively.
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