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EC number: 284-634-5 | CAS number: 84961-45-5 Extractives and their physically modified derivatives such as tinctures, concretes, absolutes, essential oils, oleoresins, terpenes, terpene-free fractions, distillates, residues, etc., obtained from Ceratonia siliqua. Leguminosae.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 05 October 2017 - 13 October 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Version / remarks:
- 22 July 2010
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Test material form:
- liquid
- Details on test material:
- - Physical appearance: Dark brown to black liquid
- Storage of test material: At room temperature
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- No correction was made for the purity/composition of the substance.
- Method: a stock solution of 10 g/L was prepared by adding 4.9924 g to 500 mL of Milli-RO water. Magnetic stirring for 19 minutes was applied to accelerate dissolution and to ensure homogeneity. Volumes of the clear dark yellow to orange stock solution (pH 5.4) corresponding to the test concentrations were then added to the test media (16 mL synthetic medium and made up to 250 mL with Milli-RO). Thereafter, 250 mL of activated sludge was added resulting in the required concentrations. Optimal contact between the test item and test organisms was ensured by applying continuous aeration and stirring.
- Controls: test medium without test item, treated in the same way as the test item solutions (blank control)
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Name and location of sewage treatment plant where inoculum was collected: 'Waterschap Aa en Maas', 's-Hertogenbosch, The Netherlands, receiving predominantly domestic sewage.
- Preparation of inoculum for exposure: the sludge was coarsely sieved (1 mm) and allowed to settle. The supernatant was removed and ISO-medium was added. A small amount of the sludge was weighed and dried overnight at ca. 105°C to determine the amount of suspended solids (3.0 g/L of sludge, as used for the test). The pH was 7.7 on the day of testing. The batch of sludge was used one day after collection; therefore 50 mL of synthetic medium (=sewage feed) was added per litre of activated sludge at the end of the collection day. The sludge was kept aerated at test temperature until use.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
Test conditions
- Test temperature:
- 20 - 22 °C
- pH:
- 7.2-7.5 (before addition of sludge), 7.2-8.2 (after the 3-hour exposure period)
- Nominal and measured concentrations:
- - Nominal concentrations: 10, 100 and 1000 mg/L
- Details on test conditions:
- TEST SYSTEM:
- Test vessel: all glass open bottles/vessels, fill volume: 500 mL
- Aeration: yes; clean, oil-free compressed air - aeration was adjusted in such a way that the dissolved oxygen concentration at the start was above 60-70% saturation (60% of air saturation is > 5 mg/l at 20°C) and to maintain the sludge flocs in suspension.
- No. of vessels per concentration (replicates): 3 (highest concentration) and 1 (lower concentrations)
- No. of vessels per control (replicates): 6
- No. of vessels for the reference item (replicates): 1
- Suspended solids concentration in final test solution: 1.5 g/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water purified by reverse osmosis (Millipore Corp., USA)
- Test medium: adjusted ISO medium
- Culture medium different from test medium: no
- Intervals of water quality measurement: temperature - continuously in a control vessel, pH - at the start and the end of the test period
OTHER TEST CONDITIONS
- Adjustment of pH: no
EFFECT PARAMETERS MEASURED: rate of oxygen consumption at the end of the period
RANGE-FINDING STUDY:
Since the results from the combined limit/range-finding test were adequate for determination of the effect concentrations, no further testing was performed and the combined limit/range-finding test was reported as the final test. - Reference substance (positive control):
- yes
- Remarks:
- 3.5-dichlorophenol (3,5-DCP)
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- - No EC50 value could be determined as no effects were observed at the highest tested concentration (the EC50 value exceeded the highest concentration).
- The test conditions during the exposure period were within the requirements prescribed in the study plan. - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Tested concentrations: 1.0,3.2, 10 and 32 mg/L
- The EC50 for 3,5-DCP was 6.62 mg/L, 95% CI: 3.20-10.12 mg/L - Reported statistics and error estimates:
- ECx calculation:
For the reference item, calculation of the EC50 value was based on a 3-parameter logistic cumulative distribution function (CDF) using non-linear regression analysis, with the percentages of respiration inhibition versus the logarithms of the corresponding concentrations of the reference item.
For the test item, no ECx values could be calculated.
NOEC calculation:
An effect was considered to be significant if statistical analysis of the data obtained for the test concentrations compared with those obtained in the control revealed significant inhibition of the respiration rate (Step-down Jonckheere-Terpstra Test Procedure and Williams Multiple Sequential t-test Procedure for the first and second full test, respectively, with: α=0.05, one-sided, smaller).
Calculations were performed with ToxRat Professional v. 3.2.1. (ToxRat Solutions® GmbH,
Any other information on results incl. tables
Table 1 Results - Respiration Rate/Inhibition and pH Values
Replicate |
Concentration (mg/L) |
pH |
Respiration rate |
% Inhibition respiration rate (mean value) |
||
Start |
End |
(mg O2/L.h) |
(mg O2/g.h)¹ |
|||
C 1 |
0 |
7.5 |
8.0 |
33.10 |
22.07 |
|
C 2 |
0 |
7.5 |
7.9 |
31.42 |
20.95 |
|
C 3 |
0 |
7.4 |
7.9 |
29.48 |
19.65 |
|
C 4 |
0 |
7.4 |
7.9 |
29.62 |
19.75 |
|
C 5 |
0 |
7.4 |
7.8 |
26.99 |
17.99 |
|
C 6 |
0 |
7.4 |
7.9 |
26.66 |
17.77 |
|
C Mean |
|
|
|
29.55 |
19.70 (RC) |
|
SD |
|
|
|
2.49 |
1.66 |
|
CV (%) |
|
|
|
8 |
8 |
|
|
|
|
|
|
|
|
R 1 |
1.0 |
7.5 |
8.1 |
28.24 |
18.83 |
4.42 |
R 2 |
3.2 |
7.5 |
8.2 |
17.16 |
11.44 |
41.92 |
R 3 |
10 |
7.5 |
8.2 |
12.34 |
8.23 |
58.23 |
R 4 |
32 |
7.5 |
8.2 |
6.49 |
4.33 |
78.03 |
|
|
|
|
|
|
|
T 1 |
10 |
7.4 |
8.0 |
25.31 |
16.87 |
14.33 |
T 2 |
100 |
7.4 |
7.8 |
35.54 |
23.69 |
-20.29 |
T 3a |
1000 |
7.2 |
7.9 |
26.57 |
17.71 |
10.07 |
T 3b |
1000 |
7.2 |
7.9 |
30.24 |
20.16 |
-2.35 |
T 3c |
1000 |
7.2 |
7.7 |
39.75 |
26.50 |
-34.54 |
T3 Mean |
|
|
32.19 |
21.46 (RT) |
-8.94 (IT) |
|
|
|
|
|
|
|
|
TA |
1000 |
7.2 |
7.2 |
0.00# |
0.00# |
100.00 |
C: Control; R: Reference item, 3,5-dichlorophenol; T: Test item, Carob Bean Extract; TA: Abiotic control of Carob Bean Extract; SD: Standard deviation; CV: Coefficient of variation
¹ The amount of suspended solids in the final test mixture was 1.5 g/L.; RC: Total respiration in the control; RT: Total respiration with Carob Bean Extract; IT: % inhibition of total respiration relative to RC
# No respiration, therefore expressed as 0 mg O2/L.h (see paragraph 6.1)
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- See 'Overall remarks'
- Conclusions:
- In a respiration inhibition test, Carob Bean Extract was not toxic to waste water bacteria at and below a concentration of 1000 mg/L (NOEC). The EC50 for inhibition of total respiration exceeded the highest tested concentration (>1000 mg/L).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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