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EC number: 271-676-4 | CAS number: 68603-84-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Test performed in 2001.
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- GLP compliance:
- yes
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): n-Heptanoic acid
- Substance type: acid
- Physical state: Liquid
- Analytical purity: >97.7%
- Lot/batch No.: FHH02 - Analytical monitoring:
- yes
- Details on sampling:
- The test medium was sampled 1 mL at a time 4 times during the exposure period and before and after water replacement for each test vessel in all testing groups, and analysed by GC after analysis pre-processing. The tested substance of each test fluid was quantitated from the ratio with the standard solution by the internal standard fluid addition method.
Test solutions - Vehicle:
- no
- Details on test solutions:
- Elendt M4 medium (according to OECD 211 guideline)
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Source: Ministry of the Environment, National Institute of Environmental Studies (presently the National Institute for Environmental Studies)
- Feeding during test: yes
- Food type: Chlorella vulgaris
- Amount: 0.2 mg C per daphnia per day
- Frequency: daily
ACCLIMATION
- Acclimation period: 6 weeks
- Acclimation conditions (same as test or not): yes - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Post exposure observation period:
- None
- Hardness:
- Not specified
- Test temperature:
20.0-20.6°C (min-max)- pH:
6.1-8.2- Dissolved oxygen:
from 4.3 to 8.8- Salinity:
- None
- Conductivity:
- Not measured
- Nominal and measured concentrations:
Nominal concentrations: 0 ; 5 ; 10 ; 20 ; 40 and 80 mg/L. See table 1 for measured concentrations. Nominal concentrations were used for the derivation of the effect concentrations.- Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 mL glass beaker covered by a Teflon sheet
- Renewal rate of test solution (frequency/flow rate): semi-static, 3 times a week
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
TEST MEDIUM / WATER PARAMETERS
Elendt M4
OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: 16L:8D
- Light intensity: 800 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): parental mortality, cumulative larvae production - Reference substance (positive control):
- no
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 40 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 20 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other:
- Remarks:
- Time to first brood
- Details on results:
- All daphnia died at the highest concentration (80 mg/L). No adverse effect was observed on cumulative larval production until the end of the experiment in the other treatments, except larval production was significantly delayed in the 40 mg/L group. Therefore NOEC was considered as 20 mg/L.
- Results with reference substance (positive control):
- None
- Reported statistics and error estimates:
The 21-day cumulative juveniles per surviving parent for each test vessel was calculated, and the presence of a significant difference between each testing group and the control group was derived by statistical methods and the maximum no observable effect concentration (NOEC) and the lowest observable effect concentration (LOEC) were determined. As the result of Bartlett’s test for homogeneity of variances showed no homogeneity, the Kruskal-Wallis test as well as the non-parametric Dunnett’s multiple comparison test were performed. In the statistical analysis, Yukms Software Statlight “#4 multi-group comparison” (Yukms Corp., Tokyo) was used.- Validity criteria fulfilled:
- yes
- Conclusions:
- Daphnia magna was exposed to graded concentrations (0 ; 5 ; 10 ; 20 ; 40 and 80 mg/L) of n-heptanoic acid for 21 days. Adult mortality, cumulative juvenile production and time to first brood were examined. The most stringent outcome was time to first brood with a LOEC of 40 mg/L. Therefore, a NOEC of 20 mg/L was considered.
All validity criteria were fulfilled.
Reference
Table 1: Analytical measurements of test item concentrations at days 0, 1, 7, 8, 15, 16 and 17 of the experiment.
Days | 0 | 1 | 7 | 8 | 15 | 16 | 16 | 17 |
Nominal concentrations (mg/l) | New | Old | New | Old | New | Old | New | Old |
Control | <0.2 | <0.2 | <0.2 | <0.2 | <0.2 | <0.2 | <0.2 | <0.2 |
5 | 4.5 | 4.7 | 4.6 | 2.6 | 3.8 | 1.5 | 4.1 | 1 |
10 | 9.8 | 11.3 | 8.5 | 8.4 | 7.5 | 5.6 | 8.4 | 6.8 |
20 | 21.1 | 20.7 | 18 | 17.4 | 13.8 | 13.2 | 16.1 | 15.1 |
40 | 41.1 | 40.4 | 42 | 41.8 | 35.3 | 34.8 | 38.6 | 40.3 |
80 | 75.6 | 80 | * | * | * | * | * | * |
* All daphnia dead
Table 2: Time to first broods (in days)
Nominal concentrations (mg/l) | ||||||
Vessel | Control | 5 | 10 | 20 | 40 | |
1 | 8 | 9 | 8 | 8 | 11 | |
2 | 8 | 8 | 8 | 8 | 8 | |
3 | 8 | 8 | 7 | 8 | 11 | |
4 | 8 | 8 | 8 | 8 | 8 | |
5 | 8 | 8 | 8 | 8 | 11 | |
6 | 8 | 8 | 8 | 8 | 11 | |
7 | 8 | 8 | 8 | 8 | 11 | |
8 | 9 | 9 | 8 | 8 | 8 | |
9 | 8 | 8 | 8 | 7 | 8 | |
10 | 8 | 8 | 8 | 8 | 11 | |
Mean | 8.1 | 8.2 | 7.9 | 7.9 | 9.8* | |
S.D. | 0.32 | 0.42 | 0.32 | 0.32 | 1.55 |
* Significantly different from the control
Description of key information
Heptanoic acid (one of the constituents of the target UVCB substance: Daphnia magna: 21-d NOEC: 18 mg/L (OECD 211; NITE, 2009)
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 18 mg/L
Additional information
The chronic toxicity to aquatic invertebrates of the constituent heptanoic acid was tested in a reproduction test according to OECD 211. The 21 -d NOEC was determined to be 18 mg/L, the EC50 47 mg/L (NITE, 2009).
Chronic toxicity is therefore not expected.
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