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EC number: 207-332-7 | CAS number: 463-04-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Parameter followed for biodegradation estimation:
- O2 consumption
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 95.7
- Sampling time:
- 28 d
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- Percentage of biodegradation at 28 days : 95.7 %
Conclusion : Readily biodegradable - Executive summary:
The objective of this study was to assess the aerobic ready biodegradability of test item by measurement of manometric respirometry over a 28-day period according to the OECD 301F guideline (July 1992).
The test item, at approximately 100 mg/L, as the sole source of carbon and energy, was incubated in a buffer-mineral salts medium which was inoculated with a mixed population of micro-organisms. The consumption of oxygen was determined from the change in pressure in the apparatus. Evolved carbon dioxide was absorbed in a solution of sodium hydroxide. The amount of oxygen taken up by the microbial population during biodegradation of the test substance (corrected for uptake by blank inoculum, run in parallel) was expressed as a percentage of ThODNH3.
As the test item is poorly soluble in test conditions and volatile, the test solutions were prepared by direct addition of the test item on the silica gel.
The study is valid since:
Based on a biodegradation rate higher than 60% in 14 days (experimental value: 78.9 and 69.1 % in 6 days) in the reference control, the test was carried out under good conditions.
At the end of the test, measurements of Biological Oxygen Demand (BOD) in two replicates of the test item solution did not show a difference of more than 20% (experimental value: 8.3 %).
The oxygen uptake of the control was not greater than 60 mg/l in 28 days (experimental value: 33.1 and 27.0 mg O2/L).
Based on a biodegradation rate higher than 25% in 14 days (experimental value: 97.4 % in 13 days) in the toxicity control, the test item was not toxic to micro-organisms.
Results are the following:
Percentage of biodegradation at 28 days : 95.7 %
Conclusion : Readily biodegradable
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- SOURCE AND TARGET CHEMICAL(S)
The read-across has been performed between pentyl nitrite (CAS No. 463-04-7; target chemical) and isoamyl nitrite (CAS No. 110-46-3; source chemical). The RA is based on an experimental study performed on the source substance with a purity of 98.3% in accordance with the OECD TG 301F and under GLP conditions.
ANALOGUE APPROACH JUSTIFICATION
The source substance and the target substance have the same chemical formula (C5H11NO2) and belong to the alkyl nitrites family. Like other alkyl nitrites, chemical and biological properties are mainly due to the nitrite group. Both target and source substances have similar physico-chemical properties.
For all these reasons, it is expected that the registered substance pentyl nitrite will have similar environmental fate than the source substance isoamyl nitrite. The read-across approach between both alkyl nitrite substances is therefore considered relevant for this endpoint.
Isoamyl nitrite was found to be readily biodegradable with a biodegradation rate of 95.7% at 28 days. The study was performed according to the OECD TG 301F under GLP conditions and all validity criteria were successful. This experimental study was therefore considered acceptable.
Because of the high biodegradation rate of the source substance under the conditions of the study and the similarity between both target and source substances, it is expected that pentyl nitrite would be also found readily biodegradable if a test was performed. - Reason / purpose for cross-reference:
- read-across source
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 95.7
- Sampling time:
- 28 d
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- Based on a read-across on an experimental study performed on another alkyl nitrite substance (isoamyl nitrite) in accordance with the OECD TG 301F and under GLP conditions, the registered substance pentyl nitrite is expected to be readily biodegradable.
- Executive summary:
For that endpoint, a read-across was performed between pentyl nitrite (CAS No. 463-04-7; target chemical) and isoamyl nitrite (CAS No. 110-46-3; source chemical).
The source substance and the target substance have the same chemical formula (C5H11NO2) and belong to the alkyl nitrites family. Like other alkyl nitrites, chemical and biological properties are mainly due to the nitrite group. Both target and source substances have similar physico-chemical properties.
For all these reasons, it is expected that the registered substance pentyl nitrite will have similar environmental fate than the source substance isoamyl nitrite. The read-across approach between both alkyl nitrite substances is therefore considered relevant for this endpoint.
An experimental study performed on the source substance isoamyl nitrite was available, and was conduted according to the OECD TG 301F under GLP conditions.
In this study, the test item, at approximately 100 mg/L, as the sole source of carbon and energy, was incubated in a buffer-mineral salts medium which was inoculated with a mixed population of micro-organisms. The consumption of oxygen was determined from the change in pressure in the apparatus. Evolved carbon dioxide was absorbed in a solution of sodium hydroxide. The amount of oxygen taken up by the microbial population during biodegradation of the test substance (corrected for uptake by blank inoculum, run in parallel) was expressed as a percentage of ThODNH3.
As the test item was poorly soluble in test conditions and volatile, the test solutions were prepared by direct addition of the test item on the silica gel.
The study is considered valid since:
Based on a biodegradation rate higher than 60% in 14 days (experimental value: 78.9 and 69.1 % in 6 days) in the reference control, the test was carried out under good conditions.
At the end of the test, measurements of Biological Oxygen Demand (BOD) in two replicates of the test item solution did not show a difference of more than 20% (experimental value: 8.3 %).
The oxygen uptake of the control was not greater than 60 mg/l in 28 days (experimental value: 33.1 and 27.0 mg O2/L).
Based on a biodegradation rate higher than 25% in 14 days (experimental value: 97.4 % in 13 days) in the toxicity control, the test item was not toxic to micro-organisms.
Results were the following for the source substance:
Percentage of biodegradation at 28 days : 95.7 %
Conclusion : Readily biodegradable
Because of the high biodegradation rate of the source substance under the conditions of the study and the similarity between both target and source substances, it is expected that pentyl nitrite would be also found readily biodegradable if a test was performed.
Referenceopen allclose all
Description of key information
Based on a read-across on an experimental study performed on another alkyl nitrite substance (isoamyl nitrite) in accordance with the OECD TG 301F and under GLP conditions, the registered substance pentyl nitrite is expected to be readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
- Type of water:
- freshwater
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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