[No public or meaningful name is available]

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

June-October 2009

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2009

Report date:

2009

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2-Amino-5-cyano-N,3-dimethylbenzamide

Cas Number:

890707-29-6

Molecular formula:

C10H11N3O

IUPAC Name:

2-Amino-5-cyano-N,3-dimethylbenzamide

Test material form:

solid: particulate/powder

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 120 mg/L
Analytical confirmation was performed at test initiation and termination.

Test solutions

Vehicle:

yes

Details on test solutions:

The 120 mg/L nominal limit test solution was prepared by adding approximately 0.480 grams of test material to 0.040 mL of N,N-dimethylformamide and Elendt M4 medium in a 500 ml glass beaker (final volume 400 mL), and sonicate for approximately 30 minutes. The maximum solvent (N,N-dimethylformamide) concentration in the solvent control and limit test solution was 0.1 mL/L. The test solution was turbid throughout the test.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Age at study initiation: Neonates, less than 24 hours old
- Feeding during test: No

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

206 mg/L (prior to start of the test)

Test temperature:

20.1 to 20.4°C

pH:

pH ranged from 7.49 to 8.24

Dissolved oxygen:

Dissolved oxygen concentrations ranged from 8.24 to 8.76 mg/L

Conductivity:

474 μs/cm (prior to start of the test)

Nominal and measured concentrations:

Nominal concentration: 120 mg/L;
Mean measured concentration: 114.85 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass beakers (100-mL) containing approximately 50 mL of test solution
- Aeration: No
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4 medium prepared according to Annex III of OECD test guideline 202
- Intervals of water quality measurement: Dissolved oxygen concentration, pH, and temperature were measured at test start and at 48 hours

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light and 8 hours darkness
- Light intensity: 700 – 750 Lux

EFFECT PARAMETERS MEASURED: Mobility was tested daily

VEHICLE CONTROL PERFORMED: yes

RANGE-FINDING STUDY
- Test concentrations: 7.5, 15, 30, 60 and 120 mg/L test substance (nominal concentrations)
- Results used to determine the conditions for the definitive study: No immobility or sublethal effects were observed in the daphnids exposed to dilution water control, solvent control (0.1 mL/L N,N dimethylformamide), and all test item concentrations after 48 hours of exposure

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 114.85 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

act. ingr.

Basis for effect:

mobility

Details on results:

Exposure of daphnids to the mean measured limit test concentration of the test substance at 114.85 mg/L resulted in 0% immobility, at the end of 48 hours. No immobility or sublethal effects were observed in the dilution water control and solvent control (0.1 mL/L N,N dimethylformamide) daphnids.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 48-hour EC50 value, based on the mean measured limit test concentration of the test substance and immobility, was greater than 114.85 mg/L.

Executive summary:

The acute toxicity of the test substance to unfed Daphnia magna neonates less than 24 hours old at test start, was determined in an unaerated, static 48-hour limit test. The test was conducted in accordance with OECD Guideline 202. Treatments consisted of a dilution water control, a solvent control (0.1mL/L N,N dimethylformamide), and one nominal limit test concentration of 120 mg/L test substance. The 48-hour EC50 for Daphnia magna based on immobility and the mean measured limit test concentration was greater than 114.85 mg/L test substance.