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EC number: 948-544-6 | CAS number: 2141947-89-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 18 May 2018 to 17 Jul 2018
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- Non-GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- other: ISO 14669
- Version / remarks:
- ISO International Standard 14669. "Water quality - Determination of acute lethal toxicity to marine copepods (Copepoda, Crustacea)", First edition 1999-04-01.
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: OECD 23
- Version / remarks:
- Guidance document on aquatic toxicity testing of difficult substances and mixtures, OECD series on testing and assessment number 23, 2000.
- Deviations:
- no
- GLP compliance:
- no
- Remarks:
- Screening study completed - o evaluate the test item for its ability to generate acute lethal toxic effects on marine copepod Tisbe battagliai during an exposure period of 48 hours.
Test material
- Reference substance name:
- 4-[3-formyl-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenoxy]benzonitrile
- EC Number:
- 948-544-6
- Cas Number:
- 2141947-89-7
- Molecular formula:
- C20H20BNO4
- IUPAC Name:
- 4-[3-formyl-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenoxy]benzonitrile
- Test material form:
- solid: particulate/powder
Constituent 1
- Specific details on test material used for the study:
- Purity/Composition: 99.73%
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- 2.2.2. Test Samples
Test samples were stored in the freezer (£ -15°C). Storage stability of samples under these conditions was demonstrated in project 20141828.
On the day of analysis, the test samples were thawed at room temperature. The samples were diluted in a 9:1 (v:v) ratio with acetonitrile and analyzed. If necessary, the samples were further diluted with 10/90 (v/v) acetonitrile/natural seawater to obtain concentrations within the calibration range. All solutions containing the test item were protected from light.
2.2.3. Preparation of Solutions
All solutions containing the test item were protected from light.
Stock and Spiking Solutions
Stock solutions of the test item were prepared in acetonitrile at concentrations of 1000 and 2000 mg/L.
Spiking solutions were made up from a stock solution and/or dilutions of this solution. The solvent of the spiking solutions was acetonitrile.
Calibration Solutions
Six calibration solutions in the concentration range of 0.08 – 20 mg/L were prepared from two stock solutions. The end solution of the calibration solutions was 10/90 (v/v) acetonitrile/natural seawater.
Quality Control (QC) Samples
4.5 mL blank medium was spiked with the test item at a target concentration of 0.1 or 100 mg/L. The QC samples were treated similarly as the test samples (see paragraph 2.2.2 Test Samples’). Blank QC samples consisting of blank medium were treated similarly to the QC and test samples.
2.2.4. Sample Injections
Calibration solutions were injected in duplicate. Test samples and QC samples were analyzed by single injection.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- 4.8.2.1. Test Concentrations
PF-06961030 Solutions containing 4.6, 10, 22, 46 and 100% of the SS prepared at a loading rate of 100 mg/L
Test organisms
- Test organisms (species):
- other aquatic crustacea: Tisbe battagliai (Copepoda, Crustacea) (Volkmann-Rocco),
- Details on test organisms:
- Source Guernsey Sea Farms Ltd., Guernsey, United Kingdom.
Reason for selection This system has been selected as an internationally accepted invertebrate species.
Characteristics 6 ± 2 days old copepodids
Study design
- Test type:
- static
- Water media type:
- saltwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Test temperature:
- 18-22°C, constant within 2°C.
- pH:
- Between 6.0 and 8.5.
- Dissolved oxygen:
- dissolved oxygen concentration >80% of the air saturation value at the start of the test, >= 4 mg/L at the end of the test.
- Salinity:
- 35‰ at start of test
- Nominal and measured concentrations:
- 4.6, 10, 22, 46 and 100% of the SS prepared at a loading rate of 100 mg/L.
- Details on test conditions:
- Test duration 48 hours
Test type Static
Test vessels 24-well plastic plates covered with a lid.
Volume of vessel 3 ml
Medium 10 μm filtered and UV treated natural marine water of the same origin as the water that was used for culturing the organisms. Water was obtained from Guernsey Sea Farms Ltd., Guernsey, United Kingdom and stored at 4ºC until test initiation. Specifications: dissolved oxygen concentration >80% of the air saturation value, pH = 8, Salinity = 35‰.
Number of copepodids 20 for each control and test item concentration
Loading 5 per vessel containing 2.5 ml of test solution
Light The study was performed in the dark (max. 1 lux)
Temperature 18-22°C, constant within 2°C.
Oxygen concentration >= 4 mg/L at the end of the test.
Aeration No aeration of the test solutions.
pH Between 6.0 and 8.5.
Feeding No feeding
Introduction of copepodids Within 53 minutes after preparation of the test solutions. - Reference substance (positive control):
- yes
- Remarks:
- Zinc sulfate heptahydrate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 12 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 11 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- The NOEC was determined with Fisher’s Exact Binomial Test with Bonfferoni Correction. The NOEC was determined to be 4.6 mg/L.
- Results with reference substance (positive control):
- At the end of the test, 50% mortality was observed in the reference control (see Table 2). Since the effect in this treatment should be between 20 and 80% it was concluded that the batch copepodids used showed normal sensitivity to zinc sulphate heptahydrate.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In conclusion, under the conditions of the present study with Tisbe battagliai the 48h-LC50 was 11 mg/L based on analytically confirmed nominal concentrations (95% confidence interval between 9.3 and 14 mg/L). The NOEC was determined to be 4.6 mg/L.
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