Fatty acids, lanolin, lithium salts

Partition coefficient

Link to relevant study record(s)

Reference

Endpoint:

partition coefficient

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 117 (Partition Coefficient (n-octanol / water), HPLC Method)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method A.8 (Partition Coefficient - HPLC Method)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

HPLC method

Partition coefficient type:

octanol-water

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: SOAP012-2
- Expiration date of the lot/batch: 1 November 2022

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Ambient

Analytical method:

high-performance liquid chromatography

Type:

log Pow

Partition coefficient:

< 1

Temp.:

25 °C

Remarks on result:

not determinable because of methodological limitations

Remarks:

The soluble components of the test item co-eluted with formamide, therefore it was assumed that the partition coefficients of the soluble components of the test item were less than that of the shortest eluting standard assessed (acetanilide) with a log10 Pow value less than 1.0

Details on results:

The soluble components of the test item co-eluted with formamide (Internal deadtime standard (IDS).

It was therefore concluded that although the method was not strictly applicable to the test item, it is logical to assume that the partition coefficients of the soluble components of the test item were less than that of the shortest eluting standard assessed (acetanilide) with a log10Pow value less than 1.0. Furthermore, it could be concluded that the larger molecular weight components, which were not soluble in water, would have much higher Pow values.

Conclusions:

The soluble components of the test item were determined to have a log10 Pow values less than 1.0. The higher molecular weight components, which were not soluble in water, would have much higher Pow values.

Executive summary:

A study to determine the partition coefficient of the test item, fatty acids lanolin lithium salts, was conducted according to the OECD 117 guideline (Dunning 2018). The experimental determination of partition coefficient is not applicable to surface active materials; however, an attempt was made to assess the property by the HPLC method since often the retention time of a material can provide a good indication of the partition coefficient. The partition coefficient was determined by the HPLC method. Six reference compounds were selected to have log10 Pow values between 1 and 6.2. An attempt was made to prepare a solution of the test item, however, a solvent was not found which could dissolve the entire test item. Consequently the decision was taken to saturate a portion of water with the test item, recognising that the resulting solution would primarily contain the six components of lowest molecular weight.

The soluble components of the test item co-eluted with the Internal deadtime standard (formamide). It was therefore concluded that the partition coefficients of the soluble components of the test item were less than that of the shortest eluting standard assessed (acetanilide) with a log10 Pow value less than 1.0. Furthermore, it could be concluded that the higher molecular weight components, which were not soluble in water, would have much higher Pow values.

The study is GLP-compliant and was conducted according to the standard guideline, therefore is considered reliable without restrictions and suitable as a key study for this endpoint.

Description of key information

The soluble components of the test item were determined to have a log10 Pow values less than 1.0. The higher molecular weight components, which were not soluble in water, would have much higher Pow values.

Additional information

A study to determine the partition coefficient of the test item, fatty acids lanolin lithium salts, was conducted according to the OECD 117 guideline (Dunning 2018). The experimental determination of partition coefficient is not applicable to surface active materials; however, an attempt was made to assess the property by the HPLC method since often the retention time of a material can provide a good indication of the partition coefficient. The partition coefficient was determined by the HPLC method. Six reference compounds were selected to have log10 Pow values between 1 and 6.2. An attempt was made to prepare a solution of the test item, however, a solvent was not found which could dissolve the entire test item. Consequently the decision was taken to saturate a portion of water with the test item, recognising that the resulting solution would primarily contain the six components of lowest molecular weight.

The soluble components of the test item co-eluted with the Internal deadtime standard (formamide). It was therefore concluded that the partition coefficients of the soluble components of the test item were less than that of the shortest eluting standard assessed (acetanilide) with a log10 Pow value less than 1.0. Furthermore, it could be concluded that the higher molecular weight components, which were not soluble in water, would have much higher Pow values.

The study is GLP-compliant and was conducted according to the standard guideline, therefore is considered reliable without restrictions and suitable as a key study for this endpoint.