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EC number: 204-854-7 | CAS number: 127-65-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to terrestrial plants
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to terrestrial plants: short-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2007
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- GLP test according to protocol
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 208 (Terrestrial Plants Test: Seedling Emergence and Seedling Growth Test)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- The concentration of the stock solution and the control was analysed.
- Vehicle:
- no
- Details on preparation and application of test substrate:
- - Substrate characteristics: A 2:l mixture of natural soil (LUFA 2.2) and quartz sand with a C-content of 1.18 %. The soil has been stored at the test facility at green house conditions until use.
Certified LUFA soil No. 2.2 (batch: Sp221107)
loamy sand (IS, acc. to German DIN classification)
≤ 2mm
Carbon content: 2.36 ± 0.22
5.6 ± 0.4
Cation exchange: 11 ± 2
Origin: LANDWIRTSCHAFTLICHE UNTERSUCHUNGS- UND FORSCHUNGSANSTALT
SPEYER (LUFA), Obere Langgasse 40, D-67346 Speyer.
Dorentruper crystal quartz sand 12 a 0.063 - 0.5 mm
Origin: DORENTRUP QUARZ GMBH CO. KG, An der Sandgrube 1, - Species:
- Avena sativa
- Plant group:
- Monocotyledonae (monocots)
- Details on test organisms:
- - Origin: LOCHOW PETKUS GMBH, Postfach 1197, D-29296 Bergen
- Storage: The seeds were stored dark at the test facility at room temperature (20 ± 10 °C) and protected from moisture until use.
- Sowing: Test containers were filled each with the same amount of treated soil (see application technique) and seeding holes were made with a seedling pistil. One seed was given in each hole and covered with soil. During cultivation the test container were bottom watered and fertilized as necessary. - Species:
- Brassica napus
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Origin: LOCHOW PETKUS GMBH, Postfach 1197, D-29296 Bergen
- Storage: The seeds were stored dark at the test facility at room temperature (20 ± 10 °C) and protected from moisture until use.
- Sowing: Test containers were filled each with the same amount of treated soil (see application technique) and seeding holes were made with a seedling pistil. One seed was given in each hole and covered with soil. During cultivation the test container were bottom watered and fertilized as necessary. - Species:
- Glycine max (G. soja)
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Origin: SAATBAU LINZ, A-4021 Linz, Schimmerstrasse 19, P.O.B. 317
- Storage: The seeds were stored dark at the test facility at room temperature (20 ± 10 °C) and protected from moisture until use.
- Sowing: Test containers were filled each with the same amount of treated soil (see application technique) and seeding holes were made with a seedling pistil. One seed was given in each hole and covered with soil. During cultivation the test container were bottom watered and fertilized as necessary. - Test type:
- seedling emergence toxicity test
- Study type:
- laboratory study
- Substrate type:
- other: mixture of natural soil and quartz sand (2:1)
- Limit test:
- no
- Total exposure duration:
- 21 d
- Test temperature:
- 17-26 °C
- Moisture:
- Relative humidity: 50-89%
- Details on test conditions:
- TEST CONDITIONS
- Method of application: The control and test item were applied once at the beginning of the study. The test item was weighed out and diluted with the required water amount. The test item solution was mixed carefully with the soil with a mixer to ensure a homogeneous distribution of the test item.
The treated soil was transferred to the test container and the seeds were sown.
- Light regime Nominal: > 3500 Ix, actual: 4362 ± 495
- Observations: day 7, 14 and 21
- Watering of the plants: Watering from the bottom as necessary
TEST SYSTEM
- Container type: Plastic container (standard flower pots), B 12 cm
- Number of replicates: 8
- Numbers of plants per replicate per dose: 5
- Date of planting: 2007-04-1 8
- Date of test substance application: 2007-04-18
- Date of phytotoxicity rating: Day 7, 14, 21 - Nominal and measured concentrations:
- Nominal: 1000 - 333 - 111 - 37 - 12.3 mg/kg soil dry weight (DW)
For soybean 4.1 mg/kg DW was tested additionally. - Reference substance (positive control):
- no
- Species:
- Glycine max (G. soja)
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 111 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: shoot height
- Species:
- Glycine max (G. soja)
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- 316 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: shoot height
- Remarks on result:
- other: 95% C.L. 264-370
- Details on results:
- see attached table
- Reported statistics and error estimates:
- NOEL: of biomass Determination of the NOEL for inhibition of shoot height, fresh weight and rate of emergence by One Way Analysis of Variances (ANOVA), followed by Dunnet’s method if statistically significant differences compared to control replicates were found.
ED-values and confidence and ranges of growth: ED25 and ED50 values were calculated by sigmodial dose-response regression. Calculation of the confidence intervals for and values were carried out using standard procedures according to Clopper and Pearson (1934). - Validity criteria fulfilled:
- yes
- Conclusions:
- Test item related phytotoxic effects were observed for the plant species oats at test concentrations ≥ 111 mg/kg and for plant species rape and soybean at concentrations ≥ 333 mg/kg
- Executive summary:
The phytotoxicity of the test item Halamid@ (batch number 0608681224) to three terrestrial plant species was determined in a seedling emergence test over a period of 21 days, from April 18 to May 09, 2007 according to OECD 208 (July 2006) at DR.U.NOACK-LABORATORIEN, 31157 Sarstedt, Germany. The test was conducted with the nominal concentrations 1000 - 333 - 111 - 37 - 12.3 mg/kg soil dry weight (DW) and for the plant species soybean 4.1 mg/kg soil dry weight (DW) was tested additionally. Test systems were one monocotyledon, oats (Gramineae), and two dicotyledons, rape (Brassicaceae) and soybean (Leguminosae). The test item was incorporated into the soil in which the seeds were sown. The test container were bottom watered with nutrient solution as needed. The toxic effects of the test item were determined on day 7, 14 and 21 by visual observations (number of emerged seedlings and phytotoxic effects) and on day 21 by shoot fresh weight and shoot height determination. The inhibition of shoot height, shoot fresh weight and rate of emergence was calculated in relation to the untreated control.
After 21 days test item related phytotoxic effects were observed for the tested plant species at the tested concentrations. The NOELs of the test item, as well as the statistically determined ED25 - and ED50- values, are summarized in the attached tables.
Reference
Description of key information
The phytotoxicity of the test item Halamid to three terrestrial plant species was determined in a seedling emergence test over a period of 21 days, according to OECD 208 (July 2006). The test was conducted with the nominal concentrations 1000, 333, 111, 37, 12.3 mg/kg soil dry weight (DW) and for the plant species soybean 4.1 mg/kg soil dry weight (DW) was tested additionally. Test systems were one monocotyledon, oats (Gramineae), and two dicotyledons, rape (Brassicaceae) and soybean (Leguminosae). The test item was incorporated into the soil in which the seeds were sown. The test container were bottom watered with nutrient solution as needed. The toxic effects of the test item were determined on day 7, 14 and 21 by visual observations (number of emerged seedlings and phytotoxic effects) and on day 21 by shoot fresh weight and shoot height determination. The inhibition of shoot height, shoot fresh weight and rate of emergence was calculated in relation to the untreated control. After 21 days test item related phytotoxic effects were observed for the tested plant species at the tested concentrations. Test item related phytotoxic effects were observed for the plant species oats at test concentrations ≥ 111 mg/kg and for plant species rape and soybean at concentrations ≥ 333 mg/kg
Key value for chemical safety assessment
- Short-term EC50 or LC50 for terrestrial plants:
- 316 mg/kg soil dw
- Long-term EC10, LC10 or NOEC for terrestrial plants:
- 111 mg/kg soil dw
Additional information
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