Fatty acids, C16-18 (even numbered), reaction products with diethylene triamine, di-Me sulfate quaternized

Water solubility

Link to relevant study record(s)

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2009-10-12 to 2009-12-07

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

test procedure in accordance with generally accepted scientific standards and described in sufficient detail

Qualifier:

no guideline followed

Principles of method if other than guideline:

- Principle of test: RP-HPLC method with charged aerosol detection (CAD)
- Short description of test conditions: hydrogenated tallow/nortallow based IQAC is a mixture of components the determination of the water solubility was
referred to the particular main components. For the determination of the water samples these three substances were analysed. The quantification was based on an external standard calibration derived from the test sample described above. The total water solubility of hydrogenated tallow/nortallow based IQAC was calculated as their sum. Three 5L glass aspirator bottles were rinsed prior to use with a mixture 1:1 methylene chloride : acetone followed by petrolether 30/40. The bottles were dried by a gentle nitrogen flow and rinsed three times with Millipore Type I water. A 4 cm glass-coated stir bar was placed in each bottle. The bottles were placed on magnetic stir plates and filled each with 5L Millipore Type I water. The tests were performed at approximately 22°C. The water was stirred quiescently with no visible vortex (< 90 rpm). After 24 h equilibration time about 250 mg test substance is added to each bottle. Samples were taken after 24 h, 12d , and 14d. Prior sampling the mixing was stopped 1h before. A 150 – 200 ml aliquot was removed from the vessels. The first 100 ml were discarded and the rest was collected in Nalgene bottles. As it was impossible to separate the water phase by centrifugation the solutions were filtrated under pressure with PORAFIL ® PE 0,05 m (part no. 671005047). Beaker, filter, and filtration unit were pre-conditioned with sample solution to avoid sample adsorption. The clear water phases were analysed by HPLC.
- Parameters analysed / observed: Recovery of the test item in water determined by HPLC-CAD.

GLP compliance:

no

Type of method:

other: slow stirring method (Letinski et al, 2002, Chemosphere 48)

Key result

Water solubility:

< 0.5 mg/L

Conc. based on:

act. ingr.

Remarks:

C16/C16 imidazolinium quat

Incubation duration:

14 d

Temp.:

22 °C

Remarks on result:

other: pH was not reported

Key result

Water solubility:

< 0.5 mg/L

Conc. based on:

act. ingr.

Remarks:

C16/C18 imidazolinium quat

Incubation duration:

14 d

Temp.:

22 °C

Remarks on result:

other: pH was not reported

Key result

Water solubility:

< 0.5 mg/L

Conc. based on:

act. ingr.

Remarks:

C18/C18 imidazolinium quat

Incubation duration:

14 d

Temp.:

22 °C

Remarks on result:

other: pH was not reported

Table 2: Results of the HPLC-CAD

Day	1			12			14
	W75H 1-1	W75H 1-1	W75H 1-1	W75H 1-1	W75H 1-1	W75H 1-1	W75H 1-1	W75H 1-1	W75H 1-1
C 16 /C 16 imidazolinium quat	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.
C 16 /C 18 imidazolinium quat	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.
C 18 /C 18 imidazolinium quat	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.

Conclusions:

The water solubility of hydrogenated tallow/nortallow based IQAC is considered to be < 0.5 mg/L at 22°C.

Executive summary:

In the present study a HPLC-CAD was performed in order to determine the water solubility of hydrogenated tallow/nortallow based IQAC. Hydrogenated tallow/nortallow based IQAC is composed of three constituents and none of the constituents was detected after 24h, 12 days and 14 days above the detection limits for each constituent. Thus, the water solubility of hydrogenated tallow/nortallow based IQAC is considered to be < 0.5 mg/L at 22°C.

Description of key information

- Determination of water solubility using HPLC-CAD

Key value for chemical safety assessment

Water solubility:

0.1 mg/L

at the temperature of:

22 °C

Additional information

In the present study a HPLC-CAD was performed in order to determine the water solubility of hydrogenated tallow/nortallow based IQAC. Hydrogenated tallow/nortallow based IQAC is composed of three constituents and none of the constituents was detected after 24h, 12 days and 14 days above the detection limits for each constituent. Thus, the water solubility of hydrogenated tallow/nortallow based IQAC is considered to be < 0.5 mg/L at 22°C.