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EC number: 287-722-1 | CAS number: 85567-10-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017-04-08 to 2017-05-02
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- 1992
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: control and 100 mg/L
- Sampling method: taken in 24 h intervals from fresh and aged test solutions. For each sampling also a retain sample was taken.
- Sample storage conditions before analysis: stored deep frozen (10 mL with 0.05 mL formic acid) - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: stock solution S1: weighed into a volumetric flask and added test medium up to the bench mark then homogenised by shaking.
- Evidence of undissolved material: the solution was clear and yellow. - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: zebrafish
- Source: own stock breeding of the lab, originally purchased from the Karlsruhe Institute of Technology
- Method of breeding: fed with commercial fish flake food (TetraMin) and protein-enriched pellet food (Caviar) at least once per day
- Maintenance of the brood fish: continuously reared in the lab
FEEDING DURING TEST
- Frequency: none - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 232 mg CaCO3/L
- Test temperature:
- 21.7 – 22.6 °C
- pH:
- 7.13 – 7.88
- Dissolved oxygen:
- 98 - 104 % of air saturation
- Conductivity:
- 698 µS/cm
- Nominal and measured concentrations:
- Nominal: 100 mg/L
Measured (geometric mean): 79.4 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 2 L glass beaker
- Fill volume: filled with 2 L of test solution
- Aeration: continuous aeration of the test tanks with a membrane pump using a Pasteur pipette
- Renewal of test solution: every 24 hours
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: max. loading of 0.19 g fish/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted test water consisting of analytical grade salts dissolved in purified water with a ratio of Ca:Mg and Na:K of 4:1 and 10:1, respectively
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours daily
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Biological Assessment: effects and mortality assessed at 0, 4, 24, 48, 72 and 96 hours after test start; all fish were euthanized, weighed and measured at the end of test.
TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 0.1, 1.0, 10.0, 100 mg/L
- Results used to determine the conditions for the definitive study: No mortality occurred up to 100 mg/L. - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 79.4 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 79.4 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Mortality: No mortality was observed
- Clinical signs: No sublethal effects were observed
- Length and weight: The average weight of the test organisms was 0.0546 ± 0.0191 g; the average length was 18.2 ± 2.3 mm.
- Analytical Results: The measured content of test in fresh test solutions was between 94 % and 103 % of nominal and 52 -77 % of nominal in aged test solutions - Reported statistics and error estimates:
- All toxicological endpoints were evaluated using nominal and measured concentrations.
- Sublethal observations / clinical signs:
Biological results:
% mortality of fish in the main test:
Test item concentration nominal/ measured [mg/L]
Control
Nominal: 100 mg/L
Measured: 79.4 mg/L
Time [h]
Mortality (%)
0
0
0
4
0
0
24
0
0
48
0
0
72
0
0
96
0
0
Analytical results:
Determined concentrations of test item during the test:
Test item
nominal
[mg/L]
Sampling
Test item
Geometric mean
Test item concentration
measured
[mg/L][%]
[%]
[mg/L]
0
0 h fresh
n.d.
-
-
-
24 h aged
n.d.
-
24 h fresh
n.d.
-
48 h aged
n.d.
-
48 h fresh
n.d.
-
72 h aged
n.d.
-
72 h fresh
n.d.
-
96 h aged
n.d.
-
100
0 h fresh
100
100
79.4
79.4
24 h aged
56.6
57
24 h fresh
102
102
48 h aged
77.2
77
48 h fresh
94
94
72 h aged
51.7
52
72 h fresh
103
103
96 h aged
66.1
66
- Validity criteria fulfilled:
- yes
- Conclusions:
- The LC50 (96 h) of the test item was determined to be > 100 mg/L (test item nominal) and > 79.4 mg/L (test item measured). The corresponding NOEC (mortality) (96 h) was 100 mg/L test item (nominal) and 79.4 mg/L test item (measured), respectively. No sublethal effects were observed in the control and at the limit test concentration of 100 mg/L (nominal) and 79.4 mg/L (measured) after 96 h.
- Executive summary:
The effects of the test item on the mortality of the Zebrafish Danio rerio were determined under worst-case exposure conditions according to OECD 203. The test item was evaluated in a semi-static limit test with daily water renewal,with one test item concentration (100 mg/L, nominal) and one control with untreated test medium. The test concentration and control contained seven fish. The test duration was 96 hours. Assessments on effects and mortality after 0, 4, 24, 48, 72 and 96 hours were conducted.
The test concentration was analytically verified. The measured content of test in fresh test solutions was between 94 % and 103 % of nominal and 52 -77 % of nominal in aged test solutions. Therefore, the effect concentrations were also evaluated based on measured concentrations, more precisely, geometric mean of the measured concentrations.
The LC50 (96 h) of the test item was determined to be > 100 mg/L (test item nominal) and > 79.4 mg/L (test item measured, geometric mean). The corresponding NOEC (mortality) (96 h) was 100 mg/L test item (nominal) and 79.4 mg/L test item (measured, geometric mean), respectively. No sublethal effects were observed in the control and at the limit test concentration of 100 mg/L (nominal) and 79.4 mg/L (measured) after 96 h.
Reference
Description of key information
For this endpoint only one test is available. The effects of the test item on the mortality of the Zebra fish Danio rerio were determined according to OECD 203. The test item was evaluated in a semi-static limit test with daily water renewal. One test item concentration (100 mg/L, nominal) and one control with untreated test medium were used.
The LC50 (96 h) of the test item was determined to be > 100 mg/L (test item nominal), which corresponds to > 79.4 mg/L measured, geometric mean. The corresponding NOEC (mortality) (96 h) was 100 mg/L test item (nominal) and 79.4 mg/L test item (measured, geometric mean), respectively.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 79.4 mg/L
Additional information
For this endpoint, one test were the mortality of the Zebrafish Danio rerio was determined according to OECD 203 is available.The test item was evaluated in a semi-static limit test with daily water renewal. One test item concentration (100 mg/L, nominal) and one control with untreated test medium was used. The test concentration and control contained seven fish each. The test duration was 96 hours. Assessments of effects and mortality after 0, 4, 24, 48, 72 and 96 hours were conducted.
The LC50 (96 h) of the test item was determined to be > 100 mg/L (test item nominal), which corresponds to > 79.4 mg/L measured, geometric mean. The corresponding NOEC (mortality) (96 h) was 100 mg/L test item (nominal) and 79.4 mg/L test item (measured), respectively. No sublethal effects were observed in the control and at the limit test concentration of 100 mg/L (nominal) and 79.4 mg/L (measured, geometric mean) after 96 h.
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