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EC number: 610-641-3 | CAS number: 512802-65-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 16/04/1993 to 17/06/1993
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- Measurement of exposure concentrations was not included. Some study details are absent from the study report.
- Qualifier:
- according to guideline
- Guideline:
- other:
- Version / remarks:
- 88/302/EEC, inhibition of cell proliferation in the green alga Scenesdesmus subsicatus 1984.
Also stated according to OECD 1984. - Principles of method if other than guideline:
- The study was carried out according to the Guideline 88/302/EEC, which was based on the exposure of green alga Scenesdesmus subsicatus to various concentrations of the test substance over a 72 hour time period. Inhibition of cell proliferation was measured in order to assess inhibition of growth.
- GLP compliance:
- yes
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 40050951 (batch number)
- Purity: dry residue 99.97%, C: 73.14% 7 mol% glycerol; 45 mole%fatty acid derivative; 48 mol% of propylene oxide.
- Expiration date of the lot/batch: Not provided
- Purity test date: Not provided
- Appearance: yellowish, liquid
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: not stated
- Stability under test conditions: not stated
- Solubility and stability of the test substance in the solvent/vehicle: not stated
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: not stated
- Analytical monitoring:
- no
- Details on sampling:
- Limited detailed provided on sampling.
pH measured at beginning and end of the study. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: test substance added to deionised water and stirred for 18 hours. The solution was filtered and the DOC content determined at 120.2 mg/L, correspoding to a product concentration of 164.3mg/L. This served as the stock solution.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): None
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): Not stated - Test organisms (species):
- Scenedesmus sp.
- Details on test organisms:
- TEST ORGANISM
- Species name: Scenedesmus subspicatus
- Strain: (CHODAT (86.81 SAG))
- Source (laboratory, culture collection): Sourced from a culture from the Institute of Water, Soil and Water Air Hygiene, Berlin.
- Age of inoculum (at test initiation): not stated
- Method of cultivation: From a stock culture, 3 days before the start of the experiment, a subculture was prepared at a cell density of about 20,000 cells / ml.
- Apparatus for culture: The breeding of the algae takes place in sterile ventilated Erlenmeyer flasks on light desks.
ACCLIMATION: No details provided. - Test type:
- not specified
- Water media type:
- not specified
- Limit test:
- no
- Total exposure duration:
- 72 h
- Remarks on exposure duration:
- cell concentrations measured at 0, 24, 48 and 72 hours.
- Hardness:
- Not stated
- Test temperature:
- 24 ± 12 ˚C in a constant temperature room
- pH:
- 8.2 - 8.4 (beginning of the test), 8.0 - 9.4 (end of the test)
- Dissolved oxygen:
- Not stated
- Salinity:
- Not stated
- Conductivity:
- Not stated
- Nominal and measured concentrations:
- Nominal concentrations: 5.9, 9.9, 16.4, 27.9, 49.3, 82.2, 147.9 mg/L.
The concentrations refer to the anyhydrous product. - Details on test conditions:
- TEST SYSTEM
- Test vessel: not stated
- Material, size, headspace, fill volume: not stated
- Aeration: not stated
- Type of flow-through (e.g. peristaltic or proportional diluter): not stated
- Renewal rate of test solution (frequency/flow rate): not stated
- Initial cells density: not stated
- Control end cells density: not stated
- No. of organisms per vessel: not stated
- No. of vessels per concentration (replicates): unclear from report, between 5 and 8
- No. of vessels per control (replicates): unclear from report, between 5 and 8
GROWTH MEDIUM: no details provided
TEST MEDIUM / WATER PARAMETERS: no details provided
OTHER TEST CONDITIONS
- Sterile test conditions: not stated
- Adjustment of pH: not stated
- Photoperiod: not stated
- Light intensity and quality: about 8000 lux, white
- Salinity (for marine algae): not stated
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: photometrically. Measured by adsorption at 685nm.
- Reference substance (positive control):
- not specified
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 49.3 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- cell number
- Details on results:
- It is stated in the report that due to high egg turbidity, the results from the higher concentrations were not taken into account in the evaluation.
The results on the cell numbers, percentage inhibition and growth curves are included in Tables 1 and 2 below. Limited effects were evident for all concentrations in terms of cell numbers, apart from at the highest concentration of 147.9 mg/L. - Results with reference substance (positive control):
- Not data
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The toxicity of propoxylated palm oil to Scenedesmus subspicatus species was assessed. A 72 hour NOEC was determined at > 49.3 mg/L, however the test substance is not toxic to aquatic organisms at the limit of solubility.
- Executive summary:
A reliable, Klimisch 2 study was carried out to assess the toxicity of propoxylated palm oil on the algal species, Scenedesmus subspicatus. The study was carried out according to the Guideline 88/302/EEC, inhibition of cell proliferation in the green alga Scenesdesmus subsicatus 1984. Toxicity was measured at the nominal concentrations: 5.9, 9.9, 16.4, 27.9, 49.3, 82.2, 147.9 mg/L. Analytical determination of actual measured concentrations is not included in the study. The cell numbers were measured at 0, 24, 48 and 72 hour time periods, and used to determine the growth curve and subsequent inhibition of growth.
The results on the cell numbers, percentage inhibition and growth curves are included in Tables 1 and 2. Limited effects were evident in these parameters, apart from at the highest concentration of 147.9 mg/L. However, it is stated in the report that due to high egg turbidity, the higher concentrations were not taken into account in the results evaluation. The 72 hour NOEC was determined at > 49.3 mg/L, although the test substance is not toxic to aquatic organisms at the limit of solubility.
Reference
Table 1. Mean values of cell numbers.
Concentration |
Mean cell numbers |
|||
Time (hours) |
||||
0 |
24 |
48 |
72 |
|
Control |
2 |
2 |
26 |
102 |
5.9 |
2 |
5 |
22 |
96 |
9.9 |
2 |
6 |
24 |
104 |
16.4 |
2 |
6 |
27 |
114 |
27.9 |
2 |
4 |
27 |
118 |
49.3 |
2 |
4 |
33 |
119 |
82.2 |
2 |
12 |
29 |
94 |
147.9 |
2 |
16 |
38 |
53 |
Comment: Due to the high turbidity in the higher concentrations the product is above the NOEC value in the algae growth inhibition test.
Table 2. Areas under the growth curves for Scenedesmus subsgicatus, inhibition rates and pH values. Concentrations refer to the anhydrous product.
|
Control |
5.9 |
9.9 |
16.4 |
27.9 |
49.3 |
82.2 |
147.9 |
Area under the growth curve |
74 |
70 |
77 |
85 |
85 |
91.5 |
93 |
75.5 |
Area under growth curve (% of control) |
|
5.4 |
-4.1 |
-14.9 |
-14.9 |
-23.6 |
-25.7 |
-2 |
Inhibition rate |
1.311 |
1.29 |
1.317 |
1.3848 |
1.359 |
1.362 |
1.283 |
1.092 |
Inhibition rate (% of control) |
|
1.6 |
-0.5 |
-2.8 |
-3.7 |
-3.9 |
2.1 |
16.7 |
pH value in test conditions 0 hours |
8.3 |
8.4 |
8.3 |
8.4 |
8.3 |
8.2 |
8.2 |
8.2 |
pH value in test conditions 72 hours |
9.4 |
9.3 |
9.3 |
9.2 |
9.2 |
9.0 |
8.6 |
8.0 |
Description of key information
A reliable, Klimisch 2 study was carried out to assess the toxicity of propoxylated palm oil on the algal species, Scenedesmus subspicatus. The 72 hour NOEC was determined at > 49.3 mg/L. Based on this result, a 72 hour EC50 of >49.3mg/L has calculated, although the test substance is not toxic to aquatic organisms at the limit of solubility.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 49.3 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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