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EC number: 209-639-1 | CAS number: 589-18-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017-05-15 to 2017-05-29
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
- Version / remarks:
- 1992
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-A (Determination of the "Ready" Biodegradability - Dissolved Organic Carbon (DOC) Die-Away Test)
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 835.3110 (Ready Biodegradability)
- Version / remarks:
- 1998
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of activated sludge: aerobic activated sewage sludge from the aeration stage of the local wastewater treatment plant, ARA Birs (Birsfelden / Switzerland), which treats predominantly domestic sewage
- Storage conditions: 1 day aerated at room temperature
- Preperation: The aerobic activated sewage sludge was washed three times by centrifugation, decantation of the supernatant liquid phase and resuspension of the solid material in tap water and finally in mineral medium. Aliquots of the homogenized final sludge suspension were weighed, thereafter dried and the dry weight of the suspended solids was determined.
- Pretreatment: no
- Concentration of sludge: 4 g dry material per liter
- Final concentration in mineral medium: 30 mg dry material per liter - Duration of test (contact time):
- 12 d
- Initial conc.:
- 38 mg/L
- Based on:
- DOC
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- TEST CONDITIONS
- Composition of medium: mineral medium according to testing guidelines
- Additional substrate: no
- Test temperature: 20 - 22 °C
- pH: 7.3 - 7.4
- pH adjusted: no
- Continuous darkness: no, diffuse light
TEST SYSTEM
- Culturing apparatus: 2000-mL Erlenmeyer glass vessels, cleaned with alcoholic hydrochloric acid, rinsed with ultrapure water and dried
- Number of culture flasks/concentration: 3
- Measuring equipment: DOC analyses were performed with the above filtrates in triplicate per sample using a TOC infrared gas analyzer equipped with an automatic sampler (Elementar Analysensysteme GmbH, Langenselbold, Germany).
- Test performed in open system: no, loosely covered with an aluminum cap to reduce losses by evaporation
SAMPLING
- Sampling frequency: on exposure day 0, 2, 5, 7, 9 and 12
- Sampling method: one sample of 10-12 mL was taken
- Sample preparation: filtered through a 0.45-μm filter. The first 2-3 mL of the filtrate were discarded
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes, 3 replicates
- Procedure control: yes, 3 replicates
- Toxicity control: yes, 1 replicate - Reference substance:
- benzoic acid, sodium salt
- Preliminary study:
- Prior to test start, the solubility of the test item in mineral medium was checked, and the test item was found to be soluble at a concentration of 100 mg/L. The measured dissolved organic carbon content (DOC) of this solution was 0.764 mg C/mg test item. The measured total organic carbon content (TOC) was 0.771 mg C/mg test item.
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- 98
- Sampling time:
- 12 d
- Details on results:
- In the test vessels containing the test item in inoculated mineral medium the mean concentration of dissolved organic carbon (DOC) continuously decreased from initially 28.8 mg/L on Day 0 to 0.6 mg/L on Day 12, at the end of the test. Expressed as percentage DOC removal, this represents a mean biodegradation value of 98 %. The mean biodegradation of the test item was 79 % on Day 5. Therefore, the pass level for ready biodegradability, i.e. at least 70 % removal of DOC in a 10 day window within the 28 day period of the test, was reached.
In the procedure controls, average biodegradation of the reference item sodium benzoate was 99 % by Exposure Day 12, i.e. at the end of the test, thus confirming suitability of the activated sludge (≥ 60 % degradation by Exposure Day 14).
In the toxicity control, containing both Methyl Benzyl Alcohol (corresponding to 49.5 % of total DOC) and the reference item sodium benzoate (corresponding to 50.5 % of total DOC) in inoculated mineral medium, the initial DOC concentration of 59.4 mg/L measured on Day 0 decreased to 10.7 mg/L on Day 12. Biodegradation amounted to 82 % on Exposure Day 12, i.e. at the end of the test. Thus, according to the test guidelines, the test item Methyl Benzyl Alcohol was not inhibitory to activated sludge at the tested concentration of 38 mg/L because degradation was >35% within 14 days. - Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The test item was found to be readily biodegradable and degraded completely after 12 days of exposure to activated sludge under the conditions of the conducted DOC Die-Away Test (OECD 301 A).
- Executive summary:
The test item was investigated for its ready biodegradability in a 28-Day DOC Die-Away Test according to the OECD Guideline for Testing of Chemicals, No. 301 A (1992). In accordance with the test guidelines the test was ended after 12 days since the biodegradation curve of the test item had reached a plateau over at least three determinations and was biodegraded completely. Expressed as percentage DOC removal, this represents a mean biodegradation value of 98 %. The pass level for ready biodegradability, i.e. at least 70 % removal of DOC in a 10-day window within the 28-day period of the test, was reached. In conclusion, the test item was found to be readily biodegradable under the test conditions within 12 days. In the toxicity control, containing both test item and the reference item sodium benzoate, the initial DOC decreased by 82 % within 12 days of exposure. Thus, the test item had no inhibitory effect on the activity of activated sludge microorganisms at the tested concentration of 38 mg/L.
Reference
Description of key information
The test item was found to be readily biodegradable and degraded completely after 12 days of exposure to activated sludge under the conditions of the conducted DOC Die-Away Test (OECD 301 A).
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
The test item was investigated for its ready biodegradability in a 28-Day DOC Die-Away Test according to the OECD Guideline for Testing of Chemicals, No. 301 A (1992). In accordance with the test guidelines the test was ended after 12 days since the biodegradation curve of the test item had reached a plateau over at least three determinations and was biodegraded completely. Expressed as percentage DOC removal, this represents a mean biodegradation value of 98 %. The pass level for ready biodegradability, i.e. at least 70 % removal of DOC in a 10-day window within the 28-day period of the test, was reached. In conclusion, the test item was found to be readily biodegradable under the test conditions within 12 days. In the toxicity control, containing both test item and the reference item sodium benzoate, the initial DOC decreased by 82 % within 12 days of exposure. Thus, the test item had no inhibitory effect on the activity of activated sludge microorganisms at the tested concentration of 38 mg/L.
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