Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 265-479-2 | CAS number: 65122-08-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1980-1981
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Remarks:
- non GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 981
- Report date:
- 1981
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- GLP compliance:
- not specified
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- 2-[3-(1,3-dihydro-1,3,3-trimethyl-2H-indol-2-ylidene)prop-1-enyl]-1,3,3-trimethyl-3H-indolium acetate
- EC Number:
- 265-479-2
- EC Name:
- 2-[3-(1,3-dihydro-1,3,3-trimethyl-2H-indol-2-ylidene)prop-1-enyl]-1,3,3-trimethyl-3H-indolium acetate
- Cas Number:
- 65122-08-9
- Molecular formula:
- C25H29N2.C2H3O2
- IUPAC Name:
- 2-[3-(1,3-dihydro-1,3,3-trimethyl-2H-indol-2-ylidene)prop-1-enyl]-1,3,3-trimethyl-3H-indolium acetate
- Test material form:
- liquid
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Winkelmann Borchen
- Strain: Wistar TNO W4
- Females nulliparous and non-pregnant:no data
- Age at study initiation: males 9 weeks; females 14 weeks
- Weight at study initiation: males: 135-198 g; females: 152-183 g
- Fasting period before study: not mentioned
- Housing: 5/sex/cage in Macrolon type III cages
- Diet: Altromin 1324 (Altromin GmbH, Lage) ad libitum)
- Water: tap water ad libitum
- Acclimation period: no data
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22± 1,5 °C
- Humidity (%): 60 ± 5 %
- Air changes (per hr): not indicated
- Photoperiod (hrs dark / hrs light): 12/12
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- unchanged (no vehicle)
- Doses:
- males: 0.1, 0.2, 0.25, 0.45 and 0.5 mL/kg bw
females: 0.05, 0.08, 0.1, 0.2, 0.3 and 0.5 mL/kg bw - No. of animals per sex per dose:
- 10/sex/dose
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations: several time on day 1 and 2 times daily thereafter (with the exception of weekend days 1 time)
- Body weight: on day 1, 7 and 14:
- Necropsy of survivors performed: yes of all animals
- Statistics:
- probit analysis
Results and discussion
Effect levelsopen allclose all
- Key result
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 0.213 mL/kg bw
- Based on:
- test mat.
- 95% CL:
- >= 0.164 - <= 0.261
- Key result
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 243 mg/kg bw
- Based on:
- test mat.
- Remarks on result:
- other: calculated based on density
- Key result
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 0.135 mL/kg bw
- Based on:
- test mat.
- 95% CL:
- >= 0.1 - <= 0.184
- Key result
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 154 mg/kg bw
- Remarks on result:
- other: calculation based on density
- Mortality:
- males: 0/10, 5/10, 7/10, 9/10 and 10/10 at 0.1, 0.2, 0.25, 0.45 and 0.5 mL/kg bw
females: 0/10, 2/10, 6/10, 6/10, 8/10 and 10/10 at 0.05, 0.08, 0.1, 0.2, 0.3 and 0.5 mL/kg bw
Deaths from 2 hours post dosing onwards - Clinical signs:
- general poor condition, sedation and ruffled fur at 0.2 mL/kg and above in males and at 0.08 mL/kg and above in females (ruffled fur at 0.1 and above in females)
- Body weight:
- severe decrease in body weight at and above 0.2 mL/kg in males and at and above 0.1 mg/kg in females
- Gross pathology:
- no findings in decedents and survivors
Applicant's summary and conclusion
- Interpretation of results:
- Category 3 based on GHS criteria
- Conclusions:
- The LD50 of the substance tested is 154 mg/kg bw
- Executive summary:
In an acute oral toxicity tests male and female Wistar rats received the substances at 0.1, 0.2, 0.25, 0.45 and 0.5 mL/kg bw or 0.05, 0.08, 0.1, 0.2, 0.3 and 0.5 mL/kg bw, respectively. Mortality and severe body weight loss were seen in males at 0.2 mL/kg and above and in females at 0.08 -0.1 mL/kg and above. Clinical signs reported included a general poor condition, sedation and ruffled fur. The LD50 in males is 243 mg/kg bw and in females 154 mg/kg bw. The substance needs to be classified as toxic (H301).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.