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EC number: 210-807-1 | CAS number: 623-68-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Test type:
- acute toxic class method
- Limit test:
- no
Test material
- Reference substance name:
- 2-butenoic anhydride
- EC Number:
- 210-807-1
- EC Name:
- 2-butenoic anhydride
- Cas Number:
- 623-68-7
- Molecular formula:
- C8H10O3
- IUPAC Name:
- (2E)-but-2-enoyl (2E)-but-2-enoate
- Test material form:
- liquid
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Hoechst Aktiengesellschaft, Kastengrund, SPF breeding colony
- Females (if applicable) nulliparous and non-pregnant: [yes/no] yes
- Age at study initiation: male 6-8 wk, female 8 wk
- Weight at study initiation: male mean = 182 g+-15
female mean = 173 g +- 6
- Fasting period before study: no data
- Housing: in fully air-conditioned rooms in macrolon cages (type 4) on soft wood granulate in groups of 5 animals
- Diet (e.g. ad libitum): ssniff® R/M-H (V 1534), ad libitum
- Water (e.g. ad libitum): tap water in plastic bottles, ad libitum
- Acclimation period: at least one day (breeding at identical conditions)
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 ± 3°C
- Humidity (%): 50 ± 20 %
- Air changes (per hr): no data
- Photoperiod (hrs dark / hrs light): 12 h daily
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- other: sesame oil (Oleum sesami)
- Doses:
- The following dose levels of Crotonsäureanhydrid were tested:
Dose Concentration Volume applied Number of
mg/kg b.w. in % (w/v) (ml/kg body weight) males females
1250 12.5 10 5
1600 16.0 10 5 -
1800 18.0 10 5 -
2000 20.0 10 5 5
2500 25.0 10 5 - - Control animals:
- no
- Statistics:
- The LD50, the 95 % limits of confidence and the equation of the probitline were established in male animals on the basis of the lethality rates by probit analysis.
(method of FELLER and SIDAK, programs supplied by Pharma Informatic, Hoechst Aktiengesellschaft).
Results and discussion
Effect levels
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- ca. 2 061 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- >= 1 847 - <= 2 512
- Mortality:
- Under the conditions described above, the following lethality rates were observed:
Lethality
Dose male animals female animals
mg/kg b.w. absolute relative (%) absolute relative (%)
1250 0/5 0
1600 0/5 0
1800 0/5 0
2000 4/5 80 0/5 0
2500 4/5 80 - Gross pathology:
- Macroscopic examination of the animals found dead revealed the following findings:
- liver light discoloured
- kidneys light discoloured
- diffuse reddening of the stomach mucosa
- stomach full of gas
- diffuse reddening of the intestinal tract
- diffuse reddening of the small intestine
The animals killed at the end of the observation period showed no macroscopically visible changes.
Applicant's summary and conclusion
- Interpretation of results:
- Category 5 based on GHS criteria
- Conclusions:
- Based on the results obtained in this study the median lethal dose value (LD50) of Crotonsäureanhydrid was calculated by probit analysis for the male Wistar rat to be 2061 mg/kg body weight.
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