Fatty acids tall-oil, reaction products with acrylic acid, potassium salt

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

other: 40 CFR Part 797.1400, 9/27/85, "Fish acute toxicity test" USTC Procedure PRO/FT FISH 224-7

Deviations:

no

Principles of method if other than guideline:

Not relevant

GLP compliance:

not specified

Remarks:

Only signatures for GLP test plan available, GLP for test itself unclear

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 6.25, 12.5, 25, 50 and 100 mg/l (nominal)
- Sampling method: A two milliliter aliquot of each sample solution was withdrawn for analysis

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION:
- Method: No solvent was required to prepare the test solutions
- Controls: Yes

Test organisms (species):

Pimephales promelas

Details on test organisms:

TEST ORGANISM
- Common name: Fathead minnow
- Source: Chesapeake cultures
- Age at study initiation (mean and range, SD): 28 - 31 days
- Length at study initiation (length definition, mean, range and SD): <=18 mm
- Feeding during test: No feeding during test

ACCLIMATION
- Type and amount of food: Laboratory prepared diet of commercial food (Zing/Tetramin)
- Feeding frequency: twice a day

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

Not relevant

Hardness:

70 - 88 mg/l CaCO3

Test temperature:

22 °C

pH:

7.1 - 8.0

Dissolved oxygen:

4.5 - 8.2

Salinity:

Not relevant

Nominal and measured concentrations:

Nominal: 6.25, 12.5, 25, 50 and 100 mg/l
Measured: 2.4, 4.9, 9.8, 16.3 and 41.3 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: vessel
- Material, size, headspace, fill volume: polypropylene, 2L filled with 1L test medium
- Aeration: Aerate by mixing test solutions to saturation prior to test; if dissolved oxygen falls below 40 percent saturation in any replicate during the test, supply oil free air at 100 ±10 bubbles per minute.
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: US EPA moderately-hard reconstituted water:
NaHCO3 - 96 mg/L
CaS04.2H20 - 60 mg/L
MgSO4 - 60 mg/L
KCl - 4 mg/L

- Alkalinity: 72 - 100 mg/L CaCO3
- Conductivity: 232 - 248 umhos
- Culture medium different from test medium: no
- Intervals of water quality measurement: temperature, pH, oxygen and conductivity were recorded daily

OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 hours light, 8 hours dark
- Light intensity: 50 to 100 ft candles

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): mortality, reflex loss, erratic swim are recorded daily

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2

Reference substance (positive control):

no

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

18.5 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

17 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

16 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

15 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

9.8 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Details on results:

- Mortality of control: 0%

Results with reference substance (positive control):

Not relevant

Reported statistics and error estimates:

Probit Analysis or Spearman - Karber Method when possible, otherwise, graphical interpolation

Sublethal observations / clinical signs:

For detailed results, see attached file "Results.doc".

Validity criteria fulfilled:

yes

Remarks:

Mortality in controls at 0%, oygen saturation above 60%

Conclusions:

The acute toxicity (96h-LC50) of Diacid 1550 potassium salt towards Pimephales promelas is 15 mg/L based on the active ingredient.

Executive summary:

The acute toxicity of Diacid 1550 potassium salt towards Pimephales promelas was investigated according to 40 CFR Part 797.1400, 9/27/85, "Fish acute toxicity test" USTC Procedure PRO/FT FISH 224-7 under GLP. Fish were exposed to nominal concentrations of 6.25, 12.5, 25, 50 and 100 mg/l and were observed for 96 hours. Based on measured concentrations, the 48h- and 96h-LC50 and 96h-NOEC were found to be 17, 15 and 9.8 mg/L respectively based on the active ingredient.

Description of key information

The acute toxicity (96h-LC50) of Diacid 1550 potassium salt towards Pimephales promelas is 15 mg/L based on the active ingredient.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

15 mg/L

Additional information

The acute toxicity of Diacid 1550 potassium salt towards Pimephales promelas was investigated according to 40 CFR Part 797.1400, 9/27/85, "Fish acute toxicity test" USTC Procedure PRO/FT FISH 224-7 under GLP. Fish were exposed to nominal concentrations of 6.25, 12.5, 25, 50 and 100 mg/L and were observed for 96 hours. Based on measured concentrations, the 48h- and 96h-LC50 and 96h-NOEC were found to be 17, 15 and 9.8 mg/L respectively based on the active ingredient.