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EC number: 801-282-5 | CAS number: 1034343-98-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 04 Jun 2015 to 17 Jul 2015
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 015
- Report date:
- 2015
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 420 (Acute Oral Toxicity - Fixed Dose Method)
- GLP compliance:
- not specified
- Remarks:
- no information given
- Test type:
- fixed dose procedure
- Limit test:
- yes
Test material
- Reference substance name:
- carbon
- EC Number:
- 801-282-5
- Cas Number:
- 1034343-98-0
- Molecular formula:
- Cx
- IUPAC Name:
- carbon
- Test material form:
- solid: nanoform
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- ICR
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Laboratory Animal Center of Jiangsu University (Certificate: SCXK (SU) 2013-0011)
- Weight at study initiation: 18.3 - 22.0 g
- Fasting period before study: animals fasted overnight
- Housing: housed in solid-floor polypropylene cages with sawdust bedding
- Diet (e.g. ad libitum): free access to food (Rat and Mouse Common Formula Diet, supplied by Suzhou Shuangshi Laboratory Animal Feed Science Co.,Ltd. (Certificate: SUSISHENG (2009) 05032).
- Water (e.g. ad libitum): free access to drinking water
- Acclimation period:
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +/- 2 °C
- Humidity (%): 40 - 70 %
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- not specified
- Details on oral exposure:
- MAXIMUM DOSE VOLUME APPLIED: 20 mL/kg bw
- Doses:
- 5000 mg/kg bw
- No. of animals per sex per dose:
- 5000 mg/kg bw: 10 female/ 10 male mice
- Control animals:
- not specified
- Details on study design:
- - Duration of observation period following administration: 14 days (5000 mg/kg bw)
- Frequency of observations: daily
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, histopathology- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: within the first 4 hours, and thereafter at least once daily
- Other examinations performed: clinical signs
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Mortality:
- 5000 mg/kg bw: female: 0/10, male: 0/10
- Clinical signs:
- other: no sign of systemic toxicity detected
Applicant's summary and conclusion
- Interpretation of results:
- other: EU GHS criteria not met
- Conclusions:
- For the test item graphene the oral LD50 of both male and female mice is higher than 2000 mg/kg bw.
- Executive summary:
Study design
Acute oral toxicity was analysed according to the Chemical toxicity test instruction (2005), Ministry of Health of the People's Republic of China, which is in large part equivalent to method described in OECD TG 420.
Ten male and female ICR mice received doses of 5000 mg/kg bw graphene by gavage.
Results and discussion
No acute toxicity was observed at 5000 mg/kg b.w., the LD50 was found to be >2000 mg/kg b.w.
Conclusion
A classification according to Regulation (EC) 1272/2008 is not warranted.
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