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EC number: 206-169-9 | CAS number: 305-84-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 01 May - 21 Jun 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 2004
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Swiss Federal Office of Public Health, Bern, Switzerland
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: Control and 100 mg/L
- Sampling method: Samples were taken at test start and after 24 and 48 h (test end).
- Sample storage conditions before analysis: All samples were stored frozen (at -20 ± 5 °C) immediately after sampling until analysis. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: 50.04 mg of the test item were dissolved in 500 mL of test water using ultrasonic treatment for 15 min and stirring for 15 min at room temperature. After the dissolving procedure the single test concentration of 100 mg/L was a clear solution. The test medium was prepared just before the introduction of the daphnids.
- Controls: Yes
- Eluate: No
- Differential loading: Yes
- Evidence of undissolved material: No, test solutions were clear - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Feeding during test: none
- Food type: The green algae Desmodesmus subspicatus SAG or a mixture of this algal suspension and a commercial fish diet (Tetra Min® Hauptfutter; TETRA-Werke, Melle, Germany) were used.
- Others: At the start of the test, the organisms used in the test were 6-24 hours old and were not first brood progeny. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- 2.5 mmol/L corresponds to 250 as CaCO3
- Test temperature:
- 21 - 22 °C
- pH:
- 7.8 - 8.0
- Dissolved oxygen:
- 8.3 - 8.5 mg/L
- Nominal and measured concentrations:
- Control and 100 mg/L (nominal)
- Details on test conditions:
- TEST SYSTEM
Test vessel:
- Type: open (covered with glass plates)
- Material, size, headspace, fill volume: 100 mL glass beakers; with 50 mL of test medium and 50 mL headspace
- Aeration: Oxygen saturation of the test water was adjusted before test start
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ISO Test water according to OECD Guideline No. 202
- Alkalinity: 0.8 mmol/L
- Ca/mg ratio: 4:1
- Na:K ratio: 10:1
- Intervals of water quality measurement: start and end of the test medium renewal periods, the pH values, dissolved oxygen concentrations and water temperature were determined in each treatment. At the same observation dates, the appearance of the test media was visually controlled and documented at the same time.
OTHER TEST CONDITIONS
- Photoperiod: 16 h light to 8 h dark cycle with an 30 min transition period
- Light intensity: 19 and 20 μmol m-2 s-1
EFFECT PARAMETERS MEASURED: Immobilization after 24 and 48 h
RANGE-FINDING STUDY
- Test concentrations: 1.0, 10 and 100 mg/L
- Results used to determine the conditions for the definitive study: Since no statistically significant effect was determined up to 100 mg/L the main test was performed as limit test. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Other biological observations: No other adverse effects (symptoms) were observed.
- Mortality of control: 0%
- Any observations that might cause a difference between measured and nominal values: No remarkable observations were made concerning the appearance of the test media. All test media were clear solutions throughout the test.
- Other: Biological and analytical results are summarized in the tables 1-3 within the section "Any other information on results incl. tables" - Results with reference substance (positive control):
- The result of the latest positive control test in March 2017 24 h EC50: 1.2 mg/L, the test facility showed that the sensitivity of the test organisms was within the range given by the guideline (24 h EC50: 0.60-2.1 mg/L).
- Validity criteria fulfilled:
- yes
- Remarks:
- For further details please refer to “Any other information on results incl. tables”.
- Conclusions:
- In conclusion, the test item had no acute toxic effects on Daphnia magna in a 48 h static test up to the nominal concentration of 100 mg/L.
Reference
Analytical Results:
The measured concentrations of the test item in the test medium of the single test concentration of 100 mg/L were 97 and 98% of nominal value at the start and the end of the test, respectively.
Table1: Analytical Results
Sampling Day/ Sample Age |
Nominal Concentration of Test Item cnom |
Measured Concentration of Test Item x |
Sample Preparation Factor F |
Determined Concentration of Test Item c |
% of Nominal Concentration |
[d/h] |
[mg/L] |
[mg/L] |
|
[mg/L] |
[%] |
0/0 |
Control |
n.d. |
1.0 |
<LOQ |
n.a. |
(fresh) |
100 |
96.5 |
1.0 |
96.5 |
97 |
2/48 |
Control |
n.d. |
1.0 |
<LOQ |
n.a. |
(aged) |
100 |
97.9 |
1.0 |
97.9 |
98 |
n.d. = no test item detected
n.a. = not applicable
LOQ: 24.2 mg/L
Biological Results:
Table 2: Effect of test item on the mobility of Daphnia magna
Nominal Test Item Concentration |
No. of Daphnids Tested |
Immobilized Daphnids after 24 Hours |
Immobilized Daphnids after 48 Hours |
||
[mg/L] |
|
No. |
[%] |
No. |
[%] |
Control |
20 |
0 |
0 |
0 |
0 |
100 |
20 |
0 |
0 |
0 |
0 |
Table 3: Validity criteria for OECD 202
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
In the control, including the control containing the solubilising agent, not more than 10% of the daphnids should have been immobilized. |
No immobile Daphnids were observed |
Yes |
The dissolved oxygen concentration at the end of the test should be ≥ 3 mg/L in control and test vessels. |
8.3 - 8.5 mg/L |
Yes |
Description of key information
EC50 (48 h) >100 mg/L (Daphnia magna, nominal, OECD 202)
Key value for chemical safety assessment
Additional information
One experimental study is available investigating the short-term effects of the substance to aquatic invertebrates. The study was performed according to OECD 202 (GLP) with the water flea Daphnia magna. Since no effects were observed in the range-finding test, the main test was performed as limit test with a concentration of 100 mg/L. The initial and the aged measured test item concentrations were in the range of 80 to 120% of the nominal values. Therefore, the endpoints were evaluated using the nominal concentration. No immobilisation of Daphnia magna was observed at the limit concentration of 100 mg/L. Thus, the EC50 (48 h) was determined to be >100 mg/L.
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