N-(4-ethoxyphenyl)-3-hydroxy-4-[(2,4,5-trichlorophenyl)azo]naphthalene-2-carboxamide

Short-term toxicity to aquatic invertebrates

Description of key information

In the acute immobilisation test with Daphnia magna (STRAUS) the effect of the saturated solution# (100 mg/L) as limit concentration of the submission substance, was determined compliant to GLP according to OECD 202 (1999, draft) and EC Directive 92/69/EC Method C.2 (1992) (reliability category 1). The limit test was conducted under static conditions over 48 h. 20 test organisms were exposed to the limit concentration and control. No test item analysis was carried out. A reference test was carried out with potassium dichromate to determine the toxicity of the reference item. The EC50-value of the reference item of 2.07 mg/L after 24 h was within the prescribed concentration range of 1.0 to 2.5 mg/L according to AQS, DIN Guideline 38412 L 30. Water quality parameters pH-value and dissolved oxygen concentration, measured at 0 and 48 h, were determined to be within the acceptable limits. The validity criteria of the test guideline were fulfilled. At the saturated solution (100 mg/L) of the test item no biologically significant effect was determined. Neither for the control nor for the treatment replicates any immobilized daphnids could be observed.

#) Saturated solution: The maximum dissolved concentration of the test item that can be achieved under the test conditions in the test medium, acc. to OECD Series, No. 23 (2000)6.