Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 201-782-8 | CAS number: 87-90-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_environmental-fate-and-pathways.png)
Bioaccumulation: aquatic / sediment
Administrative data
Link to relevant study record(s)
- Endpoint:
- bioaccumulation in aquatic species: fish
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Principles of method if other than guideline:
- A GLP study was performed to determine cyanuric acid residues in catfish and trout filets harvested at 1, 3, 7, 14, 28 and 42 days after a single oral dose of 20 mg/kg body weight.
- GLP compliance:
- yes
- Radiolabelling:
- no
- Details on sampling:
- Thawed fish filets were cut into small pieces, which were all blended with dry ice in a blender ⁄ homogenizer with pulsed action until contents were uniform and had the consistency of a fine powder. The homogenate was allowed to degas in the freezer overnight, and then was tightly sealed and stored (-80°C) until analysis. Fortified (25 and 50 ng ⁄ mL) and un-spiked (negative control) samples of tissue homogenate from store-bought filets of farm-raised catfish and rainbow trout were extracted and analyzed with each set of depletion study samples to ensure method performance criteria for cyanuric acid residue determination.
- Test organisms (species):
- other: Ictalurus punctatus and Oncorhynchus mykiss
- Details on test organisms:
- TEST ORGANISM
- Common name: Channel catfish and rainbow trout
- Source: Bought from commercial fish farms
- Length at termination: Catfish (n = 100) were 25.3 ± 1.7 cm and trout (n = 120) were 30.2 ± 4.2 cm
- Weight at termination: Catfish (n = 100) were 195.5 ± 40.0 g and trout (n = 120) were 408.3 ± 168.9 g
- Description of housing/holding area: Catfish were housed in 200-L flow-through glass aquaria. Trout were held in 1800 L round fiberglass tanks.
- Food type: Catfish were fed a commercially available extruded diet at 35% protein, 8% fat, and 5% fiber (Burris Aquaculture, Franklinton, LA, USA). Trout were fed a pelleted commercial diet at 40% protein, 14% fat, and 4% fiber (Rangen Inc. Buhl, ID, USA).
- Amount: Catfish at 1-2% bw/day, trout 1-4% bw/day.
ACCLIMATION
- Acclimation period: 1 month before use
- Acclimation conditions: The holding systems and experimental systems were supplied with the same source of freshwater - Route of exposure:
- other: Gelatin capsules administered via intragastric feeding tubes
- Test type:
- flow-through
- Water / sediment media type:
- natural water: freshwater
- Test temperature:
- Catfish tank water: 24.3 ± 1.0°C. Trout water: 12.5 ± 1.1°C
- pH:
- Catfish tank water: 7.2 ± 0.2. Trout water: 7.0 ± 0.1
- Dissolved oxygen:
- Catfish tank water: 8.4 ± 1.3 ppm. Trout water: 9.3 ± 3.4 ppm
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 60 L flow through glass aquaria
- No. of organisms per vessel: One fish/tank. A total of 8 fish were used for each withdrawal time: six fish for each of the three chemical treatments and two control fish. - Nominal and measured concentrations:
- 20 mg/kg bw cyanuric acid (nominal concentration). Actual mean dosages calculated on the basis of bw at necropsy were 21.9 ± 2.3 mg/kg for catfish and 20.6 ± 2.1 mg/kg for trout.
- Reference substance (positive control):
- no
- Details on estimation of bioconcentration:
- The mean residue levels of cyanuric acid from each treatment group for each sample day were used to calculate the tissue half-life (t 1/2) and elimination rate constant (Kel). Calculations were carried out by regression of the semi-logarithmic concentration vs. time data using PK functions for Microsoft Excel developed by Usansky et al (Department of Pharmacokinetics and Drug Metabolism, Allergan, Irvine, CA, USA).
- Details on results:
- The highest concentration of cyanuric acid, occurred on day 1 following administration, 0.68 mg⁄ kg in catfish (highest individual) or 0.46 mg/kg (mean of 6), 2.59 mg⁄ kg in trout (highest individual) or 0.86 mg/kg (mean of 6) with mean cyanuric acid concentrations declining rapidly during the first week. Other than one trout on day 1, no fish had cyanuric acid muscle residues greater than 2.5 mg⁄ kg at any time point.
- Reported statistics:
- Cyanuric acid residues in trout and catfish following the individual dose were only statistically significant from the control on day 1 (P-value < 0.001).
- Conclusions:
- Cyanuric acid residues in all dosage groups on day 3 had decreased to a level similar to that of controls. The t1/2 for fish receiving only cyanuric acid was less than 1 day for either catfish or trout. Only 2 of 30 catfish receiving only cyanuric acid developed renal crystals (days 14 and 28)
Reference
Table 1: Cyanuric acid residues in catfish filets (mg/kg) following single 20 mg/kg bw dose
Days |
1 |
3 |
7 |
14 |
28 |
|
0.68 |
0.03 |
0.00 |
0.00 |
0.00 |
|
0.48 |
0.00 |
0.00 |
0.00 |
0.00 |
|
0.46 |
0.00 |
0.00 |
0.00 |
0.00 |
|
0.44 |
0.00 |
0.00 |
0.00 |
0.00 |
|
0.37 |
0.00 |
0.00 |
0.00 |
0.00 |
|
0.30 |
0.00 |
0.00 |
0.00 |
0.00 |
Mean |
0.46 |
0.01 |
0.00 |
0.00 |
0.00 |
SD |
0.13 |
0.01 |
0.00 |
0.00 |
0.00 |
Table 2: Cyanuric acid residues in trout filets (mg/kg) following single 20 mg/kg bw dose
Days |
1 |
3 |
7 |
14 |
28 |
|
2.59 |
0.14 |
0.01 |
0.00 |
0.00 |
|
0.75 |
0.04 |
0.00 |
0.00 |
0.00 |
|
0.68 |
0.03 |
0.00 |
0.00 |
0.00 |
|
0.47 |
0.01 |
0.00 |
0.00 |
0.00 |
|
0.41 |
0.00 |
0.00 |
0.00 |
0.00 |
|
0.26 |
0.00 |
0.00 |
0.00 |
0.00 |
Mean |
0.86 |
0.03 |
0.00 |
0.00 |
0.00 |
SD |
0.87 |
0.05 |
0.00 |
0.00 |
0.00 |
Description of key information
The log kow of CYA is - 1.31 indicating no potential to bioaccumulate. In a study to determine cyanuric acid residues in catfish and trout following oral administration of 20 mg/kg cyanuric residues had decreased to a similar level to the controls by day 3. Cyanuric acid does not have any potential to bioaccumulate.
Key value for chemical safety assessment
Additional information
The Log Kow of cyanuric acid is ≤ 3. In a supporting study in catfish and trout (Reimschuessel et al 2009) cyanuric acid was administered as a single oral dose at 20 mg/kg bw. Cyanuric acid residues in all dosage groups on day 3 had decreased to a level similar to the controls. Cyanuric acid t1/2 was less than 1 day in catfish and between 1 and 2 days in trout. Cyanuric acid has no potential to bioaccumulate.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)