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EC number: 274-352-0 | CAS number: 70161-16-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Remarks:
- of read across substance
- Justification for type of information:
- Data for the target chemical is summarized based on the structurally similar read across chemicals
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- WoE report is based on three short term toxicity study of fish for the test chemical :1.ECOSAR estimates log Kow values using the US EPA's KOWWIN Program for aquatic toxicity.Toxicity of a chemical was measured on the basis of similarity of structure to chemicals for which the aquatic toxicity has been previously measured by structure-activity relationships (SARs) program based upon the octanol /water partition coefficient (Kow). Data from ECOSAR class,neutral organics was used.2.The 48 h immobilization bioassay was performed to evaluate toxicity of test material on Daphnia magna3.Short term toxicity study to Daphnia similis was carried out for 48 hrs.
- Specific details on test material used for the study:
- - Name of test material (IUPAC name): tetrasodium 2-[[4-[[4-[(4-amino-6-chloro-1,3,5-triazin-2-yl)amino]-5-sulphonato-1-naphthyl]azo]-7-sulphonato-1-naphthyl]azo]benzene-1,4-disulphonate- Molecular formula: C29H20ClN9O12S4.4Na- Molecular weight:938.173 g/mol- Smiles notation: [Na+].[Na+].[Na+].[Na+].c1(c(cc(cc1)S(=O)(=O)[O-])\N=N\c1ccc(c2ccc(cc12)S(=O)(=O)[O-])\N=N\c1ccc(c2c(cccc12)S(=O)(=O)[O-])Nc1nc(nc(n1)N)Cl)S(=O)(=O)[O-]- InChl: 1S/C29H20ClN9O12S4.4Na/c30-27-33-28(31)35-29(34-27)32-22-10-9-20(17-2-1-3-25(26(17)22)55(49,50)51)37-36-19-7-8-21(18-12-14(52(40,41)42)4-6-16(18)19)38-39-23-13-15(53(43,44)45)5-11-24(23)54(46,47)48;;;;/h1-13H,(H,40,41,42)(H,43,44,45)(H,46,47,48)(H,49,50,51)(H3,31,32,33,34,35);;;;/q;4*+1/p-4/b37-36+,39-38+;;;;- Substance type: Organic- Physical State: Solid
- Analytical monitoring:
- not specified
- Vehicle:
- not specified
- Test organisms (species):
- other: 1 and 2 Daphnia magna 3. Daphnia similis
- Details on test organisms:
- 2.- Common name: Daphnia magna- Age at study initiation: neonatal less than 24 h- Food type: lyophilized spirulina - Feeding during test: 2 h prior to the test3.TEST ORGANISM- Common name: Daphnia similis- Strain: No data available- Source: No data available- Age at study initiation (mean and range, SD): Neonates of less than 24 h old isolated from Daphnia culture were used for the study.- Weight at study initiation (mean and range, SD): No data available- Length at study initiation (length definition, mean, range and SD): No data available- Valve height at study initiation, for shell deposition study (mean and range, SD): No data available- Peripheral shell growth removed prior to test initiation: No data available- Method of breeding: No data available- Feeding during test: No data available- Food type: No data available- Amount: No data available- Frequency: No data availableACCLIMATION- Acclimation period: No data available- Acclimation conditions (same as test or not): No data available- Type and amount of food: No data available- Feeding frequency: No data available- Health during acclimation (any mortality observed): No data availableQUARANTINE (wild caught)- Duration: No data available- Health/mortality: No data available
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 48 h
- Test temperature:
- 2) 20±1 °C, 3) 21 ± 0.3°C
- Nominal and measured concentrations:
- 2) 100 mg/l ,
- Details on test conditions:
- 2) - Test vessel: exposure chamber- No. of organisms per vessel: 25 organisms3)TEST SYSTEM- Test vessel: Screw cap tubes- Type (delete if not applicable): No data available- Material, size, headspace, fill volume: No data available- Aeration: No data available- Type of flow-through (e.g. peristaltic or proportional diluter): No data available- Renewal rate of test solution (frequency/flow rate): No data available- No. of organisms per vessel: 5 organism/vessel- No. of vessels per concentration (replicates): 4 repicates- No. of vessels per control (replicates): No data available- No. of vessels per vehicle control (replicates): No data available- Biomass loading rate: No data availableTEST MEDIUM / WATER PARAMETERS- Source/preparation of dilution water: No data available- Total organic carbon: No data available- Particulate matter: No data available- Metals: No data available- Pesticides: No data available- Chlorine: No data available- Alkalinity: No data available- Ca/mg ratio: No data available- Conductivity: No data available- Culture medium different from test medium: No data available- Intervals of water quality measurement: No data availableOTHER TEST CONDITIONS- Adjustment of pH: No data available- Photoperiod: No data available- Light intensity: No data availableEFFECT PARAMETERS MEASURED (with observation intervals if applicable): After 48 hrs of exposure, the number of immobile organisms was recorded and EC50 was calculated.TEST CONCENTRATIONS- Spacing factor for test concentrations: No data available- Justification for using less concentrations than requested by guideline: No data available- Range finding study: No data available- Test concentrations: No data available- Results used to determine the conditions for the definitive study: No data available
- Reference substance (positive control):
- yes
- Remarks:
- K2Cr2o7
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 42.8 mg/L
- Nominal / measured:
- estimated
- Conc. based on:
- test mat.
- Basis for effect:
- not specified
- Remarks on result:
- other: Neutral Organics
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 58.22 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 87 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: Confidence interval (p – 0.05) is 76-99 mg/l.
- Conclusions:
- The test chemical tetrasodium 2-[[4-[[4-[(4-amino-6-chloro-1,3,5-triazin-2-yl)amino]-5-sulphonato-1-naphthyl]azo]-7-sulphonato-1-naphthyl]azo]benzene-1,4-disulphonate is likely to be toxic to aquatic invertebrate in the concentration range of 42 - 87 mg/l
- Executive summary:
Data available for the structurally similar read across chemicals and predicted study has been reviewed to determine the short term toxicity of aquatic invertebrate of the test chemical tetrasodium 2-[[4-[[4-[(4-amino-6-chloro-1,3,5-triazin-2-yl)amino] -5-sulphonato-1-naphthyl]azo] -7-sulphonato-1-naphthyl]azo] benzene-1,4-disulphonate (70161 -16 -9).The studies are as mentioned below:
1.Based on the prediction done by EPI suite, ECOSAR version 1.1, on the basis of similarity of structure to chemicals for which the aquatic toxicity has been previously measured by structure-activity relationships (SARs) program, the LC 50 value for short term toxicity to aquatic invertebrates was predicted.
On the basis of this program, the LC 50 value for short term toxicity to aquatic invertebrates was predicted to be 42.8 mg/l for CAS nametetrasodium 2-[[4-[[4-[(4-amino-6-chloro-1,3,5-triazin-2-yl)amino]-5-sulphonato-1-naphthyl]azo]-7-sulphonato-1-naphthyl]azo]benzene-1,4-disulphonatein 48 hrs.
Based on this value it can be concluded that the substance is considered to be toxic to aquatic environment and can be classified in aquatic acute 3 category as per the criteria mentioned in CLP regulation.
2.Test material was used to evaluate short term toxicity on 24 h old neonates Daphnia magna as per OECD guideline 202. Untreated ISO formulation freshwater and potassium dichromate (0.32 – 3.2 mg/L) were used as negative and positive controls respectively. The effect concentration (LC50) with Daphnia magna at 48 h was observed to be 58.22 mg/l.
3.Short term toxicity study to Daphnia similis was carried out for 48 hrs. Neonates of less than 24 h old isolated from Daphnia culture were used for the study. Five test organisms were placed in each screw cap tubes, filled with 10 mL solution, and then incubated at 21±0.3°C in the dark. After 48 h of exposure, the number of immobile organisms was recorded. Jspear software was used to calculate the median 50% immobilization concentration (EC50). Based on immobilization of test organism by test chemical , the EC50 value was determine to be 87 mg/l along with confidence interval (p – 0.05) of 76-99 mg/l.
Thus, based on the above summarised studies, and it’s structurally similar read across substance, it can be concluded that effect concetration value is in the range of 42 - 87 mg/L. Thus, comparing this value with the criteria of CLP regulation, tetrasodium 2-[[4-[[4-[(4-amino-6-chloro-1,3,5-triazin-2-yl)amino]-5-sulphonato-1-naphthyl]azo]-7-sulphonato-1-naphthyl]azo]benzene-1,4-disulphonate can be classified as aquatic acute 3 for short term toxicity for aquatic invertebrate .
Reference
Description of key information
Short- term toxicity to aquatic invertebrate:
Data available for the structurally similar read across chemicals and predicted study has been reviewed to determine the short term toxicity of aquatic invertebrate of the test chemical tetrasodium 2-[[4-[[4-[(4-amino-6-chloro-1,3,5-triazin-2-yl)amino] -5-sulphonato-1-naphthyl]azo] -7-sulphonato-1-naphthyl]azo] benzene-1,4-disulphonate (70161 -16 -9).The studies are as mentioned below:
1.Based on the prediction done by EPI suite, ECOSAR version 1.1, on the basis of similarity of structure to chemicals for which the aquatic toxicity has been previously measured by structure-activity relationships (SARs) program, the LC 50 value for short term toxicity to aquatic invertebrates was predicted.
On the basis of this program, the LC 50 value for short term toxicity to aquatic invertebrates was predicted to be 42.8 mg/l for CAS nametetrasodium 2-[[4-[[4-[(4-amino-6-chloro-1,3,5-triazin-2-yl)amino]-5-sulphonato-1-naphthyl]azo]-7-sulphonato-1-naphthyl]azo]benzene-1,4-disulphonatein 48 hrs.
Based on this value it can be concluded that the substance is considered to be toxic to aquatic environment and can be classified in aquatic acute 3 category as per the criteria mentioned in CLP regulation.
2.Test material was used to evaluate short term toxicity on 24 h old neonates Daphnia magna as per OECD guideline 202. Untreated ISO formulation freshwater and potassium dichromate (0.32 – 3.2 mg/L) were used as negative and positive controls respectively. The effect concentration (LC50) with Daphnia magna at 48 h was observed to be 58.22 mg/l.
3.Short term toxicity study t oDaphnia similis was carried out for 48 hrs. Neonates of less than 24 h old isolated from Daphnia culture were used for the study. Five test organisms were placed in each screw cap tubes, filled with 10 mL solution, and then incubated at 21±0.3°C in the dark. After 48 h of exposure, the number of immobile organisms was recorded. Jspear software was used to calculate the median 50% immobilization concentration (EC50). Based on immobilization of test organism by test chemical , the EC50 value was determine to be 87 mg/l along with confidence interval (p – 0.05) of 76-99 mg/l.
Thus, based on the above summarised studies, and it’s structurally similar read across substance, it can be concluded that effect concetration value is in the range of 42 - 87 mg/L. Thus, comparing this value with the criteria of CLP regulation, tetrasodium 2-[[4-[[4-[(4-amino-6-chloro-1,3,5-triazin-2-yl)amino]-5-sulphonato-1-naphthyl]azo]-7-sulphonato-1-naphthyl]azo]benzene-1,4-disulphonate can be classified as aquatic acute 3 for short term toxicity for aquatic invertebrate .
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 42.8 mg/L
Additional information
Short- term toxicity to aquatic invertebrate:
Data available for the structurally similar read across chemicals and predicted study has been reviewed to determine the short term toxicity of aquatic invertebrate of the test chemical tetrasodium 2-[[4-[[4-[(4-amino-6-chloro-1,3,5-triazin-2-yl)amino] -5-sulphonato-1-naphthyl]azo] -7-sulphonato-1-naphthyl]azo] benzene-1,4-disulphonate (70161 -16 -9).The studies are as mentioned below:
1.Based on the prediction done by EPI suite, ECOSAR version 1.1, on the basis of similarity of structure to chemicals for which the aquatic toxicity has been previously measured by structure-activity relationships (SARs) program, the LC 50 value for short term toxicity to aquatic invertebrates was predicted.
On the basis of this program, the LC 50 value for short term toxicity to aquatic invertebrates was predicted to be 42.8 mg/l for CAS nametetrasodium 2-[[4-[[4-[(4-amino-6-chloro-1,3,5-triazin-2-yl)amino]-5-sulphonato-1-naphthyl]azo]-7-sulphonato-1-naphthyl]azo]benzene-1,4-disulphonatein 48 hrs.
Based on this value it can be concluded that the substance is considered to be toxic to aquatic environment and can be classified in aquatic acute 3 category as per the criteria mentioned in CLP regulation.
2.Test material was used to evaluate short term toxicity on 24 h old neonates Daphnia magna as per OECD guideline 202. Untreated ISO formulation freshwater and potassium dichromate (0.32 – 3.2 mg/L) were used as negative and positive controls respectively. The effect concentration (LC50) with Daphnia magna at 48 h was observed to be 58.22 mg/l.
3.Short term toxicity study toDaphnia similiswas carried out for 48 hrs. Neonates of less than 24 h old isolated from Daphnia culture were used for the study. Five test organisms were placed in each screw cap tubes, filled with 10 mL solution, and then incubated at 21±0.3°C in the dark. After 48 h of exposure, the number of immobile organisms was recorded. Jspear software was used to calculate the median 50% immobilization concentration (EC50). Based on immobilization of test organism by test chemical , the EC50 value was determine to be 87 mg/l along with confidence interval (p – 0.05) of 76-99 mg/l.
Thus, based on the above summarised studies, and it’s structurally similar read across substance, it can be concluded that effect concetration value is in the range of 42 - 87 mg/L. Thus, comparing this value with the criteria of CLP regulation, tetrasodium 2-[[4-[[4-[(4-amino-6-chloro-1,3,5-triazin-2-yl)amino]-5-sulphonato-1-naphthyl]azo]-7-sulphonato-1-naphthyl]azo]benzene-1,4-disulphonate can be classified as aquatic acute 3 for short term toxicity for aquatic invertebrate .
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