Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 202-223-0 | CAS number: 93-15-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 19.10.2017-28.12.2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- GLP compliance:
- yes
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Yasho Industries Pvt. Ltd. / 40003010617
- Expiration date of the lot/batch: 2019 February
- Purity test date: 2017 March
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: at room temperature
- Stability under test conditions: stable
- Solubility and stability of the test substance in the solvent/vehicle: test item formed an emulsion in mineral medium at 220 mg/L
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: not observed
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: not specified
- Preliminary purification step (if any): no - Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, domestic, adapted
- Details on inoculum:
- - Source of inoculum (e.g. location, sampling depth, contamination history, procedure): Sewage Treatment Plant Bengaluru - 560058 India
- Storage conditions: at room temperature, aerated
- Storage length: 5 days
- Preparation of inoculum for exposure: The effluent was allowed to settle for one hour, decanted and the decanted effluent was used in the test
- Initial cell/biomass concentration: 7.0 x 10^7 CFU/L
- Water filtered: no - Duration of test (contact time):
- 28 d
- Initial conc.:
- >= 10 - <= 20 mg/L
- Based on:
- TOC
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Mixture of 24 mL of stock solution A with 1920 mL of Milli-Q water, then 2.4 mL each of stock solutions B, C and D was added and finally the volume was made up to 2400 mL using Milli-Q water (See tables)
- Test temperature: 21-24 C
- pH: approx. 7.8
- pH adjusted: no
- Aeration of dilution water: with CO2 free air
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: Conical flask 5 L - each fitted with an aeration tube reaching nearly to the bottom of the flask and an outlet
- Number of culture flasks/concentration: 2 / 10-20 mg/L
- Method used to create aerobic conditions: The flasks were aerated with CO2 free air at 46 to 50 mL/minute
- Test performed in closed vessels due to significant volatility of test substance: no
- Details of trap for CO2 and volatile organics if used:
SAMPLING
- Sampling frequency: Days 2, 4, 7, 9, 11, 14, 18, 21, 25, 29
- Sampling method: the CO2 was trapped in barium hydroxide and was measured by titration of the residual hydroxide or as inorganic carbon
CONTROL AND BLANK SYSTEM
- Inoculum blank: 2 flasks
- Toxicity control: yes - Reference substance:
- benzoic acid, sodium salt
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- ca. 10.12
- St. dev.:
- 0.608
- Sampling time:
- 2 d
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- ca. 68.03
- St. dev.:
- 5.721
- Sampling time:
- 11 d
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- ca. 92.11
- St. dev.:
- 2.729
- Sampling time:
- 29 d
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- Based on the results, the test item was concluded as readily biodegradable as 68.03% mean degradation was achieved in between Days 2 and 11, crossing 60% pass level in 10-day window period after reaching 10% of ThCO2 production and within the 28-d period of the test.
- Executive summary:
The ready biodegradability of Methyl Eugenol was tested using the CO2 Evolution Test. The test item was added to two test vessels at the concentration of 66 mg/L of mineral medium (equivalent to 16.3 mg of Total Organic Carbon/L). Two control treatments containing only the inoculum, one reference item treatment and one toxicity control treatment containing the test item and the reference item were also tested. All the treatments were added with equal volume of inoculum which was collected from the secondary effluent treatment plant receiving predominantly domestic sewage.
Treatment mixtures were aerated for 29 days with carbon dioxide (CO2) free air. The CO2 released was trapped in a series of bottles containing barium hydroxide, which were connected to the outlet of each test vessel. The residual barium hydroxide was measured on Days 2, 4, 7, 9, 11, 14, 18, 21, 25 and 29 after the initiation of the test.
The mean per cent degradation of test item was 92.11% at the end of the test while, the percent degradation of reference item was 93.31% and the toxicity control was 88.16% at the end of the test. It was observed that the mean degradation of test item reached 68.03% on Day 11 after reaching 10.12% on Day 2. Therefore, the degradation of test item, Methyl Eugenol reached more than 60% pass level in 10 -day window period after reaching 10% of ThCO2 production and within the 28 -d period of the test.
The test fulfilled all the validatity criteria.
Based on the results, the test item was concluded as readily biodegradable as 68.03% mean degradation was achieved in between Day 2 and Day 11, crossing 60% pass level in 10 day window period after reaching 10% of ThCO2 production and within the 28 day period of the test.
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- (Q)SAR
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- results derived from a valid (Q)SAR model and falling into its applicability domain, with limited documentation / justification
- Justification for type of information:
- 1. SOFTWARE
Danish QSAR database
2. MODEL (incl. version number)
BIOWIN 5 and BIoWIN 6
3. SMILES OR OTHER IDENTIFIERS USED AS INPUT FOR THE MODEL
c1(OC)c(OC)cc(CC=C)cc1
4. SCIENTIFIC VALIDITY OF THE (Q)SAR MODEL
[Explain how the model fulfils the OECD principles for (Q)SAR model validation. Consider attaching the QMRF or providing a link]
- Defined endpoint: not specified
- Unambiguous algorithm: not specified
- Defined domain of applicability: not specified
- Appropriate measures of goodness-of-fit and robustness and predictivity: not specified
- Mechanistic interpretation: not specified
5. APPLICABILITY DOMAIN
[Explain how the substance falls within the applicability domain of the model]
- Descriptor domain: not specified
- Structural and mechanistic domains: not specified
- Similarity with analogues in the training set: not specified
- Other considerations (as appropriate): not specified
6. ADEQUACY OF THE RESULT
[Explain how the prediction fits the purpose of classification and labelling and/or risk assessment]
The prediction supports the findings of the test results, that the substnace is readily biodegradable. - Qualifier:
- no guideline available
- Principles of method if other than guideline:
- - Software tool(s) used including version:
Danish QSAR database
- Model(s) used: BIOWIN 5 and BIOWIN 6
- Model description: see field 'Justification for non-standard information', 'Attached justification'
- Justification of QSAR prediction: see field 'Justification for type of information', 'Attached justification' - Key result
- Parameter:
- probability of ready biodegradability (QSAR/QSPR)
- Value:
- 0.561
- Remarks on result:
- readily biodegradable based on QSAR/QSPR prediction
- Remarks:
- Biowin 5
- Key result
- Parameter:
- probability of ready biodegradability (QSAR/QSPR)
- Value:
- 0.595
- Remarks on result:
- readily biodegradable based on QSAR/QSPR prediction
- Remarks:
- Biowin 6
- Interpretation of results:
- readily biodegradable
- Conclusions:
- According to the QSAR predictions of the Danish QSAR database the substance considered readily biodegradable.
- Executive summary:
According to the QSAR predictions of the Danish QSAR database the substance considered readily biodegradable.
Referenceopen allclose all
Table 1: Carbon dioxide Production
Day | CO2 Production (mg) | Cumulative CO2 produced (mg) Test - Blank Mean |
|||||||||
Test Flasks | Blank | ||||||||||
Flask 1 | Flask 2 | Flask 5 | Flask 6 | Flask 3 | Flask 4 | Mean | Flask 1 | Flask 2 | Flask 5 | Flask 6 | |
Day 2 | 30.14 | 31.68 | 36.63 | 31.24 | 12.54 | 11.88 | 12.21 | 17.93 | 19.47 | 24.42 | 19.03 |
Day 4 | 39.49 | 34.54 | 41.25 | 31.46 | 12.98 | 14.19 | 13.59 | 43.83 | 40.42 | 52.08 | 36.9 |
Day 7 | 41.03 | 43.56 | 44.55 | 38.61 | 9.68 | 12.65 | 11.17 | 73.69 | 72.81 | 85.46 | 64.34 |
Day 9 | 37.18 | 33.33 | 41.14 | 33.55 | 13.86 | 11.77 | 12.82 | 98.05 | 93.32 | 113.78 | 85.07 |
Day 11 | 29.48 | 48.73 | 17.16 | 28.93 | 13.53 | 11.88 | 12.71 | 114.82 | 129.34 | 118.23 | 101.29 |
Day 14 | 25.19 | 10.89 | 13.75 | 17.38 | 9.9 | 11.44 | 10.67 | 129.34 | 129.56 | 121.31 | 108 |
Day 18 | 30.58 | 31.68 | 34.1 | 19.91 | 21.89 | 19.36 | 20.63 | 139.29 | 140.61 | 134.78 | 107.28 |
Day 21 | 13.64 | 10.01 | 10.34 | 41.14 | 0.88 | 1.98 | 1.43 | 151.5 | 149.19 | 143.69 | 146.99 |
Day 25 | 24.09 | 14.08 | 20.24 | 12.54 | 7.48 | 0.88 | 4.18 | 171.41 | 159.09 | 159.75 | 155.35 |
Day 29 | 5.17 | 10.56 | 3.19 | 5.83 | 10.67 | 4.95 | 7.81 | 168.77 | 161.84 | 155.13 | 153.37 |
Total CO2 produced in the inoculum blank during the test = 107.22 mg/L
= 35.74 mg/L
Note: Flask 1 and 2: Contain test item (methyl eugenol) and inoculum in mineral media
Flask 3 and 4: Contain inoculum in mineral media
Flask 5: Contain reference item (sodium benzoate) and inoculum in mineral media
Flask 6: Contain test item, reference item and inoculum in mineral media
Table 2: ph of Test Solutions
Flask No. | pH at the end of the test (Day 28) |
Flask 1 | 7.84 |
Flask 2 | 7.83 |
Flask 3 | 7.81 |
Flask 4 | 7.85 |
Flask 5 | 7.82 |
Flask 6 | 7.83 |
Note: Flask 1 and 2: Contain test item (methyl eugenol) and inoculum in mineral media
Flask 3 and 4: Contain inoculum in mineral media
Flask 5: Contain reference item (sodium benzoate) and inoculum in mineral media
Flask 6: Contain test item, reference item and inoculum in mineral media
Table 3: Degradation of Test Item and Reference Item
Day | Test Item | Refernce Item | Test Item + Reference Item | ||
Flask 1 | Flask 2 | Flask 3 | |||
Day 2 | 9.99 | 10.85 | 10.42 | 14.69 | 10.94 |
Day 4 | 24.42 | 22.52 | 23.47 | 31.33 | 21.21 |
Day 7 | 41.06 | 40.57 | 40.82 | 51.4 | 36.99 |
Day 9 | 54.64 | 52 | 53.32 | 68.44 | 48.9 |
Day 11 | 63.98 | 72.07 | 68.03 | 71.12 | 58.23 |
Day 14 | 72.07 | 72.19 | 72.13 | 72.97 | 62.08 |
Day 18 | 77.61 | 78.35 | 77.98 | 81.07 | 61.67 |
Day 21 | 84.42 | 83.13 | 83.78 | 86.43 | 84.5 |
Day 25 | 95.51 | 88.65 | 92.08 | 96.09 | 89.3 |
Day 29 | 94.04 | 90.18 | 92.11 | 93.31 | 88.16 |
% degradation = (mg CO2 produced / (mg TOC added in test x 3.67)) x 100
Note: Flask 1 and 2: Contain test item (methyl eugenol) and inoculum in mineral media
Flask 3 and 4: Contain inoculum in mineral media
Flask 5: Contain reference item (sodium benzoate) and inoculum in mineral media
Flask 6: Contain test item, reference item and inoculum in mineral media
Description of key information
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
- Type of water:
- freshwater
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.