Cesium acetate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From Jan 7, 1997 to Jan 30, 1997

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1997

Report date:

1997

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: EPA Pesticide Assessment Guidelines, Subdivision F (81-1) EPA Health Effects Testing Guidelines (TSCA Guideline No. 798.1175)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test type:

acute toxic class method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Cesium acetate

EC Number:

222-248-0

EC Name:

Cesium acetate

Cas Number:

3396-11-0

Molecular formula:

C2H4O2.Cs

IUPAC Name:

cesium acetate

Test material form:

liquid

Remarks:

Cloudy

Details on test material:

- Name of test material (as cited in study report): Cesium acetate
- Physical state: A cloudy liquid
- Storage condition of test material: Room temperature

Test animals

Species:

other: Albino rats

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

Naive, young adult male and female Sprague-Dawleyd eriveda lbino rats weighing 198-303 g were used. The animals were purchase from a vendor who equals or exceeds U.S.D.A. standards even though rats are not regulated animals. All animals were acclimated to the laboratory for at least five days before being used. Animals were housed in groups of five in wire mesh suspension cages and were supplied Teklad 4% mouse/rat diet and tap water ad libitum during both acclimation and test periods except for withholding food overnight prior to dosing. The animal room was maintained on a 12 h light/12 h dark cycle and at a temperature of 64-79°F and a relative humidity of 30-70%. There were no contaminants in either the feed or the water that were expected to affect the outcome of this study.

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

unchanged (no vehicle)

Details on oral exposure:

The test substance was administered by gavage.

Doses:

1250, 1580, 2000 and 5000 mg/kg bw

No. of animals per sex per dose:

Forty animals (five/sex/dose level)

Control animals:

no

Details on study design:

Overnight prior to dosing, food (but not water) was withheld from the rats. The time of fasting and dosing was documented. Groups of five male and five female rats received the test substance by gavage at varying dose levels so that a median lethal dose could be calculated. Four groups were used for the study. The test substance was administered undiluted using the bulk density to determine the dose volume. Individual doses were calculated using post-fast body weights.

Results and discussion

Effect levels

Key result

Sex:

male/female

Dose descriptor:

LD50

Effect level:

1 550 mg/kg bw

Based on:

test mat.

95% CL:

ca. 1 450 - ca. 1 660

Mortality:

Based on the cumulative mortality observed during the 14 d observation period following a single oral dose of undiluted test substance the acute oralLD50 value was calculated to be 1550 mg/kg bw with 95% Confidence Intervals of 1450 mg/kg bw and 1660 mg/kg bw.

Clinical signs:

other: Clinical signs noted during the observation period included varying degrees of depression, convulsions, respiratory distress, excess salivation, gross signs of distress, diarrhea, and external staining. Clinical signs were noted at all dose levels investi

Gross pathology:

Gross necropsy findings for animals that died during the observation period included those generally seen in agonal animals indications of gastro-intestinal irritation, and external staining. There were no gross pathological changes observed in animals which survived the 14 d observation period.

Applicant's summary and conclusion

Conclusions:

Under the study conditions, the acute oral LD50 value of the test substance in rats was calculated to be 1550 mg/kg bw.

Executive summary:

A study was conducted to assess the acute oral toxicity of the test substance according to OECD 401 and EPA Pesticide Assessment Guidelines, Subdivision F (81-1) EPA Health Effects Testing Guidelines (TSCA Guideline No. 798.1175), in compliance with GLP. The substance was administered undiluted to groups of five male and five female Sprague Dawley rats at dose levels of 0, 1250, 1580, 2000 and 5000 mg/kg bw. Animals were then observed for 14 d. Clinical signs included varying degrees of depression, convulsions, respiratory distress, excess salivation, gross signs of distress, diarrhea and external staining. Clinical signs were noted at all dose levels, however only faecal staining was seen at 1250 mg/kg bw. All surviving animals exhibited bodyweight gain at Day 14. Gross necropsy findings for animals that died during the observation period included those generally seen in agonal animals, i.e. indications of gastro-intestinal irritation and external staining. There were no gross pathological changes in animals which survived the 14 d observation period. Under the study conditions, the acute oral LD50 value of the test substance in rats was calculated to be 1550 mg/kg bw (Harrod, 1997).