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EC number: 947-407-8 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms, other
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Remarks:
- Data of read across substance
- Justification for type of information:
- Data for the target chemical is summarized based on the structurally similar read across chemicals
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- other: As mention below
- Principles of method if other than guideline:
- WoE report was prepared based on two toxicity studies of microorganisms for the test chemical :
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- - Name of test material : Reaction mass of Benzenamine, N,N-dimethyl- , molybdate, tungstate & phosphates- Substance type: Organic- Physical state: Solid- Physical Appearance: Violet powder
- Analytical monitoring:
- not specified
- Vehicle:
- not specified
- Test organisms (species):
- other: Agrobacterium radiobacter, A. tumefaciens, Bradyrhizobium japonicum, E. coli, Erwinia atroseptica, E. herbicola, E. uredovora, P.fluorescence, P. phaseolicola, P. syringae, Rhizobium trifolii, Xanthomonas malvacearum, X. phaseoli, X. stewartii
- Details on inoculum:
- 1 and 2 - Laboratory culture: Test organisms were taken from the collection of the Plant Protection Institute, Hungarian Academy of Sciences (Budapest, Hungary).- Name and location of sewage treatment plant where inoculum was collected: No data available- Method of cultivation: A layer of 5 mm depth of inoculated medium was dispensed into Petri dishes of 90 mm diameter. Filter paper discs of 5 mm diameter were impregnated with 1 µM solution of test compounds and placed centrally on the surface of the agar plate. Growth inhibition zones were measured after 24 h incubation at 21 ± 1 °C. After incubation colony diameters were determined and growth inhibition was calculated in percentage of dye free colony growth.- Preparation of inoculum for exposure: Bacterial suspensions for screening were prepared by washing cells with sterile tap water containing 0.3% peptone from slants of 20 h old cultures grown at (21±1) °C.- Pretreatment: No data available- Initial biomass concentration: No data availableIn 2nd study another gram positive bacteria were used: Gram – positive bacteria: Corynebacterium fascians, Corynebacterium flaccumfaciens, Corynebacterium michiganense, Corynebacterium oortii, Stapylococcus aureus, Micrococcus luteus, Bacillus subtilis and Bacillus thuringiensis Berliner subp. kurstaki
- Test type:
- not specified
- Water media type:
- not specified
- Total exposure duration:
- 24 h
- Remarks on exposure duration:
- After 24 hrs of incubation, colony diameters were determined and growth inhibition was calculated in percentage of dye free colony growth.
- Test temperature:
- 21 ± 1 °C
- Nominal and measured concentrations:
- 0.6918 mg/l (1 µM)
- Details on test conditions:
- 1. TEST SYSTEM - Test vessel: Petri dishes- Material, size, headspace, fill volume: The diameter of Petri dishes was 90 mm.EFFECT PARAMETERS MEASURED (with observation intervals if applicable): After 24 hrs of incubation, colony diameters were determined and growth inhibition was calculated in percentage of dye free colony growth. - Test concentrations: 0.6918 mg/l (1 µM)
- Reference substance (positive control):
- not specified
- Key result
- Duration:
- 24 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.692 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: In 1st study
- Key result
- Duration:
- 24 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.62 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: 1 and 2nd study
- Validity criteria fulfilled:
- not specified
- Conclusions:
- 1. Based on no effect on growth inhibition of gram negative test organisms, the NOEC value of test material was determine to be 0.6918 mg/l for Erwinia herbicola and Pseudomonas syringae. Whereas the growth inhibition was observed on the other organisms so LOEC was 0.6918 mg/l for remaining 12 bacteria.2. Based on growth inhibition of gram positive test organisms, the LOEC value of test chemical was determine to be 0.6918 mg/l. Based on above experimental studies it was concluded that the reaction mass of Benzenamine, N,N-dimethyl- , molybdate, tungstate & phosphates shows low effect at 0.6194 mg/l.
- Executive summary:
Summarized results of the data available for the structurally and functional similar read across chemicals study has been reviewed to determine the toxicity of the test reaction mass of Benzenamine, N,N-dimethyl- , molybdate, tungstate & phosphates on the growth of microorganisms. The studies are as mentioned below:
Determination of toxicity of test material on the growth of 14 gram negative microorganisms. Inhibitory activity of test chemical was determined by the traditional agar gel method. The conc. of test chemical used for the study was 0.6918 mg/l (1 µM). 14 different gram negative bact. were used for the study was Agrobacterium radiobacter, Agrobacterium tumefaciens, Bradyrhizobium japonicum, Escherichia coli, Erwinia atroseptica,Erwinia herbicola, Erwinia uredovora, Pseudomonas fluorescence, Pseudomonas phaseolicola, Pseudomonas syringae , Rhizobium trifolii, Xanthomonas malvacearum, Xanthomonas phaseoli and X. stewartii. These test organisms were taken from the collection of the Plant Protection Institute, Hungarian Academy of Sciences (Budapest, Hungary). The bacteria were maintained on Nutrient Agar (Oxoid CM3) completed with vitamins pyridoxine. HCl, thiamine. HCl, riboflavine and nicotinamide at 1, 10, 1 and 20 mg/l concentrations. Bacterial suspensions for screening were prepared by washing cells with sterile tap water containing 0.3% peptone from slants of 20 h old cultures grown at (21±1) °C. A layer of 5 mm depth of inoculated medium was dispensed into Petri dishes of 90 mm diameter. Filter paper discs of 5 mm diameter were impregnated with 1 µM solution of test compounds and placed centrally on the surface of the agar plate. Growth inhibition zones were measured after 24 h incubation at 21 ± 1 °C. After incubation colony diameters were determined and growth inhibition was calculated in percentage of dye free colony growth. Each determination was run in quadruplicate. Based on no effect on growth inhibition of gram negative test organisms, the NOEC value of test material was determine to be 0.6918 mg/l for Erwinia herbicola and Pseudomonas syringae. Whereas the growth inhibition was observed on the other organisms so LOEC was 0.6918 mg/l for remaining 12 bacteria.
Determination of toxicity of test chemical on the growth of 8 gram positive microorganisms. Inhibitory activity of test chemical was determined by the traditional agar gel method. The conc. of test chemical used for the study was 0.6918 mg/l (1 µM).
8 different gram positive bact. were used for the study-
Gram – positive bacteria:
Corynebacterium fascians
Corynebacterium flaccumfaciens
Corynebacterium michiganense
Corynebacterium oortii
Stapylococcus aureus
Micrococcus luteus
Bacillus subtilis and
Bacillus thuringiensis Berliner subp. kurstaki
These test organisms were taken from the collection of the Plant Protection Institute, Hungarian Academy of Sciences (Budapest, Hungary). The bacteria were maintained on Nutrient Agar (Oxoid CM3) completed with vitamins pyridoxine. HCl, thiamine. HCl, riboflavine and nicotinamide at 1, 10, 1 and 20 mg/l concentrations. Bacterial suspensions for screening were prepared by washing cells with sterile tap water containing 0.3% peptone from slants of 20 h old cultures grown at (21±1) °C. A layer of 5 mm depth of inoculated medium was dispensed into Petri dishes of 90 mm diameter. Filter paper discs of 5 mm diameter were impregnated with 1 µM solution of test compounds and placed centrally on the surface of the agar plate. Growth inhibition zones were measured after 24 h incubation at 21 ± 1 °C. After incubation colony diameters were determined and growth inhibition was calculated in percentage of dye free colony growth. Each determination was run in quadruplicate. Based on growth inhibition of gram positive test organisms, the LOEC value of test substance was determine to be 0.6918 mg/l.
Based on above experimental studies it was concluded that the reaction mass of Benzenamine, N,N-dimethyl- , molybdate, tungstate & phosphates shows low effect at 0.6194 mg/l.
Reference
1. Table:Microbiological activity of synthetic dyes towards bacteria.
Test organism | Growth inhibition (in %) |
Agrobacterium radiobacter | 11 |
Agrobacterium tumefaciens | 11 |
Bradyrhizobium japonicum | 28 |
Escherichia coli | 15 |
Erwinia atroseptica | 8 |
Erwinia herbicola | 0 |
Erwinia uredovora | 13 |
Pseudomonas fluorescence | 6 |
Pseudomonas phaseolicola | 13 |
Pseudomonas syringae | 0 |
Rhizobium trifolii | 11 |
Xanthomonas malvacearum | 6 |
Xanthomonas phaseoli | 19 |
X. stewartii | 9 |
Study 2: Table: Microbiological activity of test chemical toward bacterias.
Test organism | Growth inhibition (in %) |
Corynebacterium fascians
| 18 |
Corynebacterium flaccumfaciens
| 19 |
Corynebacterium michiganense
| 17 |
Corynebacterium oortii
| 13 |
Stapylococcus aureus
| 30 |
Micrococcus luteus
| 19 |
Bacillus subtilis | 20 |
Bacillus thuringiensisBerliner subp.kurstaki | 17 |
Description of key information
1. Based on no effect on growth inhibition of gram negative test organisms, the NOEC value of test material was determine to be 0.6918 mg/l for Erwinia herbicola and Pseudomonas syringae. Whereas the growth inhibition was observed on the other organisms so LOEC was 0.6918 mg/l for remaining 12 bacteria.
2. Based on growth inhibition of gram positive test organisms, the LOEC value of test chemical was determine to be 0.6918 mg/l.
Based on above experimental studies it was concluded that the reaction mass of Benzenamine, N,N-dimethyl- , molybdate, tungstate & phosphates shows low effect at 0.6194 mg/l.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 0.692 mg/L
Additional information
Summarized results of the data available for the structurally and functional similar read across chemicals study has been reviewed to determine the toxicity of the test reaction mass of Benzenamine, N,N-dimethyl- , molybdate, tungstate & phosphates on the growth of microorganisms. The studies are as mentioned below:
Determination of toxicity of test material on the growth of 14 gram negative microorganisms. Inhibitory activity of test chemical was determined by the traditional agar gel method. The conc. of test chemical used for the study was 0.6918 mg/l (1 µM). 14 different gram negative bact. were used for the study was Agrobacterium radiobacter, Agrobacterium tumefaciens, Bradyrhizobium japonicum, Escherichia coli, Erwinia atroseptica,Erwinia herbicola, Erwinia uredovora, Pseudomonas fluorescence, Pseudomonas phaseolicola, Pseudomonas syringae , Rhizobium trifolii, Xanthomonas malvacearum, Xanthomonas phaseoli and X. stewartii. These test organisms were taken from the collection of the Plant Protection Institute, Hungarian Academy of Sciences (Budapest, Hungary). The bacteria were maintained on Nutrient Agar (Oxoid CM3) completed with vitamins pyridoxine. HCl, thiamine. HCl, riboflavine and nicotinamide at 1, 10, 1 and 20 mg/l concentrations. Bacterial suspensions for screening were prepared by washing cells with sterile tap water containing 0.3% peptone from slants of 20 h old cultures grown at (21±1) °C. A layer of 5 mm depth of inoculated medium was dispensed into Petri dishes of 90 mm diameter. Filter paper discs of 5 mm diameter were impregnated with 1 µM solution of test compounds and placed centrally on the surface of the agar plate. Growth inhibition zones were measured after 24 h incubation at 21 ± 1 °C. After incubation colony diameters were determined and growth inhibition was calculated in percentage of dye free colony growth. Each determination was run in quadruplicate. Based on no effect on growth inhibition of gram negative test organisms, the NOEC value of test material was determine to be 0.6918 mg/l for Erwinia herbicola and Pseudomonas syringae. Whereas the growth inhibition was observed on the other organisms so LOEC was 0.6918 mg/l for remaining 12 bacteria.
Determination of toxicity of test chemical on the growth of 8 gram positive microorganisms. Inhibitory activity of test chemical was determined by the traditional agar gel method. The conc. of test chemical used for the study was 0.6918 mg/l (1 µM).
8 different gram positive bact. were used for the study-
Gram – positive bacteria:
Corynebacterium fascians
Corynebacterium flaccumfaciens
Corynebacterium michiganense
Corynebacterium oortii
Stapylococcus aureus
Micrococcus luteus
Bacillus subtilis and
Bacillus thuringiensis Berliner subp. kurstaki
These test organisms were taken from the collection of the Plant Protection Institute, Hungarian Academy of Sciences (Budapest, Hungary). The bacteria were maintained on Nutrient Agar (Oxoid CM3) completed with vitamins pyridoxine. HCl, thiamine. HCl, riboflavine and nicotinamide at 1, 10, 1 and 20 mg/l concentrations. Bacterial suspensions for screening were prepared by washing cells with sterile tap water containing 0.3% peptone from slants of 20 h old cultures grown at (21±1) °C. A layer of 5 mm depth of inoculated medium was dispensed into Petri dishes of 90 mm diameter. Filter paper discs of 5 mm diameter were impregnated with 1 µM solution of test compounds and placed centrally on the surface of the agar plate. Growth inhibition zones were measured after 24 h incubation at 21 ± 1 °C. After incubation colony diameters were determined and growth inhibition was calculated in percentage of dye free colony growth. Each determination was run in quadruplicate. Based on growth inhibition of gram positive test organisms, the LOEC value of test substance was determine to be 0.6918 mg/l.
Based on above experimental studies it was concluded that the reaction mass of Benzenamine, N,N-dimethyl- , molybdate, tungstate & phosphates shows low effect at 0.6194 mg/l.
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