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Diss Factsheets
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EC number: 923-900-3 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2009
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Deviations:
- no
- Principles of method if other than guideline:
- This test method is equal to OECD Guideline 209 (1984).
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- TEST ORGANISM:
- mixed population of aquatic microorganisms, origin of an aeration tank of a domestic sewage treatment plant (Municipal STP Cologne-Stammheim, Germany)
- pretreatment: aeration at 20 +/- 2 °C, daily feed with synthetic medium
- pH of the synthetic sewage feed 7.5 +/- 0.5
- test concentration of the activated sludge: 800 mg/l suspended solids - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Hardness:
- No data
- Test temperature:
- 19.6 °C (concerning the highest concentration of 1000 mg/l)
- pH:
- 7.9 (concerning the highest concentration of 1000 mg/l)
- Dissolved oxygen:
- oxygen (mg/l): O2-start 5.5; O2-end 2.9 (concerning the highest concentration of 1000 mg/l)
- Details on test conditions:
- TEST CONDITIONS:
- type of application: direct weighing
- temperature: 20 +/- 2 °C
- stirring period before start of incubation time: 16 h
- test concentration: 3 (10, 100, 1000 mg/l)
- test parameter: respiration rate
- aeration: permanent - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol, concentration (5, 10, 20 mg/l)
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: probit analysis
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: probit analysis
- Details on results:
- Test concentrations are given as nominal concentrations and were not confirmed by analytical methods. The physico-chemical oxygen consumption has been determined at 1000 mg/L test item concentration. As no physico-chemical oxygen consumption was observed at that test item concentration this observation also holds true for the lower test item concentrations.
- Results with reference substance (positive control):
- EC50 of the reference substance 3,5-dichlorophenol is in the range 5 – 10 mg/L
- Validity criteria fulfilled:
- yes
- Remarks:
- (respiratory rates of the 2 controls differ less than 15 % from each other; the EC50 of the reference compound 3,5-dichlorophenol is in the range 5–30 mg/L)
- Conclusions:
- The test item showed 0 % respiration inhibition of activated sludge at the highest test item concentration of 1000 mg/L.
- Executive summary:
In order to test the toxicity to microorganism of the substance, activated sludge from predominantly domestic sewage plant was exposed to three nominal concentrations of the substance (10, 100, 1000 mg/l) and to the solution with positive reference substance for a period of 3 hours. Dissolved oxygen was recorded and respiration rates were calculated. 0 % respiration inhibition was observed at the highest concentration of test substance. This toxicity study is classified as acceptable and satisfies the guideline requirements for the toxicity study to microorganisms according to the OECD guideline 209.
Reference
Description of key information
The substance showed 0 % respiration inhibition of activated sludge at the highest test item concentration of 1000 mg/L according to the OECD guideline 209. The EC50 is higher than 1000 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
- EC10 or NOEC for microorganisms:
- 1 000 mg/L
Additional information
"Should read: > 1000 mg/L"
In order to test the toxicity to microorganism of the substance, activated sludge from predominantly domestic sewage plant was exposed to three nominal concentrations of the substance (10, 100, 1000 mg/l) and to the solution with positive reference substance for a period of 3 hours. Dissolved oxygen was recorded and respiration rates were calculated. 0 % respiration inhibition was observed at the highest concentration of test substance. This toxicity study is classified as acceptable and satisfies the guideline requirements for the toxicity study to microorganisms according to the OECD guideline 209.
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