Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 259-627-5 | CAS number: 55406-53-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- fish short-term toxicity test on embryo and sac-fry stages
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1992-06-22
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EPA OPP 72-4 (Fish Early Life-Stage and Aquatic Invertebrate Life-Cycle Studies)
- GLP compliance:
- yes
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 9102-6111 - Analytical monitoring:
- yes
- Details on sampling:
- Sample storage conditions before analysis:
The dilution water control and the high, middle, and low test concentrations were sampled and analyzed for test item concentration twice prior to the start of the definitive exposure. During the in-life phase of the definitive study, water samples were removed from both replicate test solutions of each treatment level and the controls on test days 0, 5,12,19, 26,29, 33 and 35 for analysis of test material concentration. Three Quality Control (QC) samples were also prepared at each sampling interval and remained with the set of exposure solution samples throughout the analytical process - Vehicle:
- yes
- Remarks:
- acetone
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Stock solutions at a concentration of 9.8 mg/mL were prepared on test days -1,6,13, 20, 27 and 33 by dissolving the appropriate amount of test item with acetone (CAS #67-64-1) to a total volume of 50 mL.
- Controls: no treatment control, vehicle control
- Chemical name of vehicle: acetone
- Concentration of vehicle in test medium: maximum amount present in any test concentration (0.010 mL/L)
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): no - Test organisms (species):
- Pimephales promelas
- Details on test organisms:
- TEST ORGANISM
- Common name: fathead minnow
- Strain: brood stock maintained at Springborn Laboratories Inc.
- Age at study initiation: < 24 h
- Feeding during test: During the definitive test, subsequent to the completion of hatch (day 5), the fry were fed live brine shrimp nauplii (Memia salina) three times daily on weekdays and two times daily on weekends.
- Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 35 d
- Remarks on exposure duration:
- 30 d post hatch
- Hardness:
- 24 - 30 mg/L
- pH:
- 6.8 - 7.0
- Conductivity:
- 110 - 140 µmhos/cm
- Nominal and measured concentrations:
- nominal: 100; 50 ; 25; 12; 6.2 µg/L
measured: 57; 27; 19; 8.4; 4 µg/L - Details on test conditions:
- TEST SYSTEM
- Embryo cups: 14 glass jars (5 cm O.D., 8 cm high) with 40-mesh Nitex screen bottoms.
- Test vessel: each test aquarium measured 39 x 20 x 25 cm with a 14.5-cm high side drain that maintained a constant exposure solution volume of approximately 11 L
- Type of flow-through: proportional diluter
- Renewal rate of test solution: 6.5 aquarium volumes per 24 h, 90 % replacement time of approx. 8.5 h
- No. of fertilized eggs/embryos per vessel: 60
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- No. of vessels per vehicle control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: The dilution and control water was well water which was pumped into an epoxy-coated concrete reservoir where it was supplemented with Town of Wareham untreated well water and aerated
- Total organic carbon: Based on monthly analyses, the TOC concentration of the dilution water source was determined to be 1.1 and 0.38 mg/L for the months of September and October 1991, respectively
- Pesticides: In accordance with requirements stated within the EPA-GLP regulations, routine analyses were also conducted on representative samples from the dilution water source for the presence of pesticides and PCB's. None of these compounds have been detected at concentrations that are considered toxic in any of the water samples analyzed in agreement with US EPA and ASTM guidelines.
- Alkalinity: 19-25 mg/L
- Ca/mg ratio: 24-30 mg/L
- Culture medium different from test medium: no
- Intervals of water quality measurement: During the study, weekly characterization of the well water was performed
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light, 8 h dark
- Light intensity: 20-55 footcandles (equivalent to approx. 215.3 - 592.0 lux)
EFFECT PARAMETERS MEASURED: embryo survival at hatch, larval survival, total length and wet weight
VEHICLE CONTROL PERFORMED: yes - Reference substance (positive control):
- no
- Key result
- Duration:
- 35 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 19 µg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: length and weight
- Key result
- Duration:
- 35 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 8.4 µg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: length and weight
- Key result
- Duration:
- 35 d
- Dose descriptor:
- other: Maximum Acceptable Toxicant Concentration (MATC)
- Effect conc.:
- > 8.4 - < 19 µg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: length and weight
- Details on results:
- - Overall mortality/survival: At test termination (day 35) larval surviving ranging from 76-90 % was recorded in all test item concentrations. There were no statistical significant differences to the control in any of the treatment groups
- Length and weight of surviving fish: Statistical analysis demonstrated a significant reduction (p <0.05) in larval length in all three of these treatment level solutions when compared to the mean total length of the pooled control larvae (32.7 mm). The mean total length in the remaining concentrations (8.4 and 4.0 µg/L) was 32.7 and 33.1, respectively, and was comparable to the pooled control organism data. Similarly, the mean wet weight of organisms exposed to the three highest treatment levels (57, 27 and 19 µg/L) was 189, 266 and 284 mg, respectively, and was significantly reduced when compared to the mean wet weight of the pooled control organisms (340 mg). The mean wet weight of larvae exposed to the 8.4 and 4.0 µg/L treatment levels was 329 and 343 mg, respectively.
- Effect concentrations exceeding solubility of substance in test medium: no
- Incidents in the course of the test which might have influenced the results: no - Results with reference substance (positive control):
- not applicable
- Validity criteria fulfilled:
- yes
- Conclusions:
- In this study early life stages of fathead minnow were exposed to test item concentrations of 57; 27; 19; 8.4; 4 µg/L (measured). Body length and wet weight were found to be the most sensitive indications of the test item toxicity. A respective NOEC was determined to be 8.4 µg/L.
- Executive summary:
The objective of this study was to determine the effects of test item on fathead minnow (Pimephales promelas) embryos and larvae during continuous aqueous exposure. Test concentrations were selected based on the results of preliminary flow-through exposures of juvenile fathead minnows. The definitive embryo/larval exposure was initiated within 24 hours after egg fertilization and continued through 35 days (30 days post-hatch). The effects on embryo survival at hatch and on survival and growth (wet weight and total length) of larvae at test termination were measured and used to estimate LOEC, NOEC and the Maximum Acceptable Toxicant Concentration (MATC).
Reference
Description of key information
In a GLP and guideline compliant study, early life stages of fathead minnow were exposed to test item concentrations of 57; 27; 19; 8.4; 4 µg/L (measured). Body length and wet weight were found to be the most sensitive indications of the test item toxicity. Respective NOEC was determined to be 8.4 µg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 8.4 µg/L
Additional information
Fish toxicity test on embryo and sac-fry stages
The objective of this study was to determine the effects of test item on fathead minnow (Pimephales promelas) embryos and larvae during continuous aqueous exposure. Nominal test concentrations were 100; 50 ; 25; 12; 6.2 µg/L and were selected based on the results of preliminary flow-through exposures of juvenile fathead minnows. The definitive embryo/larval exposure was initiated within 24 hours after egg fertilization and continued through 35 days (30 days post-hatch). The effects on embryo survival at hatch and on survival and growth (wet weight and total length) of larvae at test termination were measured and used to estimate LOEC, NOEC and the Maximum Acceptable Toxicant Concentration (MATC).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.