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EC number: 821-762-8 | CAS number: 4563-56-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2002
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- No analytical measurements of test material. TOC analyticals did not show gradient
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- not specified
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Water accommodated fraction (WAFs)
Test material was added to 21 L of water and stirred for 48 hours. The stir rate was set to form a slight dimple at the water surface. After stirring, the mixture was allowed to stand for 4 hours. A wide bore glass tube, covered at one end with Parafilm was submerged into the vessel, sealed end down, to a depth of approximately 5 cm from the bottom of the vessel. The aqueous phase or WAF was removed by mid-depth siphoning to give the necessary loading rates.
- Evidence of undissolved material (e.g. precipitate, surface film, etc): Microscopic inspection of the WAFs showed no micro-dispersions or undissolved test material - Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: Rainbow trout
- Source: Brow Well Fisheries Limited, Hebden, Yorkshire, UK
- Length at study initiation (length definition, mean, range and SD): 4.3 cm
- Weight at study initiation (mean and range, SD): 1.07 g
- Food type: Commerical trout pellets. Feeding was discontinued 24 hours prior to start of the study. Food was held for 24 hours.
ACCLIMATION
- Acclimation period: Two weeks
- Acclimation conditions (same as test or not): same as test conditions - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- none
- Hardness:
- 100 mg/l as CaCO3
- Test temperature:
- 14 °C
- pH:
- 7.6 - 8.0
- Dissolved oxygen:
- 9.0 - 9.8 mg Oxygen/L
- Salinity:
- not applicable/freshwater
- Nominal and measured concentrations:
- Nominal: 1.0, 1.8, 3.2, 5.6, 10.0 mg/L
- Details on test conditions:
- TEST SYSTEM
- Type: closed
- Material, size, headspace, fill volume: 20 litre glass vessels
- Aeration: None
- Renewal rate of test solution (frequency/flow rate): Daily
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dechlorinated laboratory tap water passed through activated carbon filter
- Total organic carbon: 2 mg C/L
- Particulate matter: 0.10 mg/L
- Alkalinity: 104 mg/L as CaCO3
- Conductivity: 473 µS
OTHER TEST CONDITIONS
- Photoperiod: 16 hours light
TEST CONCENTRATIONS
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations: 1.0, 10, 100, and 1000 mg/L
- Results used to determine the conditions for the definitive study:
1.0 mg/L: 0/3
10, 100, and 1000 mg/L: 3/3 for each dose - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LL50
- Effect conc.:
- 4.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 4.4 - 4.6 mg/L
- Duration:
- 96 h
- Dose descriptor:
- NOELR
- Effect conc.:
- 3.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- Behavioural abnormalities: Loss of equilibrium in 5.6 mg/L Test group at 48 hours
- Mortality of control: 0/10 - Validity criteria fulfilled:
- yes
- Conclusions:
- LL50 (96h) = 4.4 mg/L (OECD 203, Oncorhynchus mykiss, mortality, nominal)
- Executive summary:
No data are available on the toxicity of Phosphorodithioic acid, O,O-di-dodecyl-esters, zinc salts, neutral and basic (CAS No.4563-56-8) to fish. Therefore data from the analogue substance Zinc bis[O,O-bis(2-ethylhexyl)] bis(dithiophosphate) (CAS 4259-15-8) were used for read across. This read-across is in accordance with Regulation (EC) No. 1907/2006 Annex XI, 1.5 and justified in detail in the overall summary (IUCLID Chapter 6.1) and within the analogue justification attached in IUCLID Section 13.
The short-term toxicity of Zinc bis[O,O-bis(2-ethylhexyl)] bis(dithiophosphate) (CAS 4259-15-9) (Safepharm laboratories Ltd, 2002) to fish was investigated in a study according to OECD 203 and following GLP principles (CAS 4259-15-8, Oronite, Short-term toxicity to fish). In this test, Oncorhynchus mykiss were exposed to nominal loading rates of 1.0, 1.8, 3.2, 5.6 and 10.0 mg/L under semi-static conditions. Due to a low solubility of the test item a water accommodated fraction design was performed (stirred for 48 h and allowed to stand for additional 4 h; the aqueous phase or WAF was removed by mid-depth siphoning to give the necessary loading rates). 10 fish per vessel were exposed to the test item loading for a period of 96 h. The LL50 was determined to be at 4.4 mg/L.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- Please refer to the analogue justification provided in Section 13.
- Reason / purpose for cross-reference:
- read-across source
- Duration:
- 96 h
- Dose descriptor:
- LL50
- Effect conc.:
- 4.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 4.4 - 4.6 mg/L
Referenceopen allclose all
Description of key information
LL50 (96h) = 4.4 mg/L (OECD 203, Oncorhynchus mykiss, mortality, nominal)
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 4.4 mg/L
Additional information
No data are available on the toxicity of Phosphorodithioic acid, O,O-di-dodecyl-esters, zinc salts, neutral and basic (CAS No.4563-56-8) to fish. Therefore data from the analogue substance zinc bis[O,O-bis(2-ethylhexyl)] bis(dithiophosphate) (CAS 4259-15-8) were used for read across. This read-across is in accordance with Regulation (EC) No. 1907/2006 Annex XI, 1.5 In this case of read-across, the best suited (highest degree of structural similarity, nearest physico-chemical properties) read-across substance was used for the assessment. This read-across is justified in within the analogue justification in IUCLID Section 13.
The short-term toxicity of Zinc bis[O,O-bis(2-ethylhexyl)] bis(dithiophosphate) (CAS 4259-15-9) (Safepharm laboratories Ltd, 2002) to fish was investigated in a study according to OECD 203 and following GLP principles (CAS 4259-15-8, Oronite, Short-term toxicity to fish). In this test, Oncorhynchus mykiss were exposed to nominal loading rates of 1.0, 1.8, 3.2, 5.6 and 10.0 mg/L under semi-static conditions. Due to a low solubility of the test item a water accommodated fraction design was performed (stirred for 48 h and allowed to stand for additional 4 h; the aqueous phase or WAF was removed by mid-depth siphoning to give the necessary loading rates). 10 fish per vessel were exposed to the test item loading for a period of 96 h. The LL50 was determined to be at 4.4 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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