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EC number: 606-729-6 | CAS number: 212386-71-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2009
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Remarks:
- Screening assay (only results were reported, limited number of strains, methodological details are missing)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2009
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- not applicable
- GLP compliance:
- no
- Remarks:
- Screening test
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- (4-ethoxy-2,3-difluorophenyl)boronic acid
- EC Number:
- 606-729-6
- Cas Number:
- 212386-71-5
- Molecular formula:
- C8 H9 B F2 O3
- IUPAC Name:
- (4-ethoxy-2,3-difluorophenyl)boronic acid
- Test material form:
- solid
Constituent 1
Method
- Target gene:
- HIS operon (S. thyphimurium)
TRP operon (E. coli)
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 98
- Species / strain / cell type:
- S. typhimurium TA 100
- Species / strain / cell type:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- Liver S9 mix from Aroclor 1254-pretreated rats with standard co-factors
- Test concentrations with justification for top dose:
- 1st series (TA 98, TA 100, E. coli WP2 uvr A): 5, 15.8, 50, 158, 500, 500, 1580 and 5000 μg/plate
2nd series (TA 100, E. coli WP2 uvr A): 500, 889, 1580, and 2810 μg/plate - Vehicle / solvent:
- DMSO
Controlsopen allclose all
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 4-nitroquinoline-N-oxide
- sodium azide
- other: daunomycin
- Remarks:
- without S9 mix
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene
- Remarks:
- with S9 mix
- Details on test system and experimental conditions:
- The assessment of test material-induced effects is dependent on the number of spontaneous revertants of each bacterial strain (solvent controls) and the increase in the number of revertants at the test material concentration which shows the highest number of colonies.
- Rationale for test conditions:
- Screening assay
- Statistics:
- n.a.
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- reduced background lawn at 5000 µg/plate
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- True negative controls validity:
- not examined
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- reduced background lawn at 5000 µg/plate
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- True negative controls validity:
- not examined
- Positive controls validity:
- valid
- Key result
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- reduced background lawn at 5000 µg/plate
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- True negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
- Precipitation and time of the determination: Precipitation at the beginning of the experiment starting at 1580 µg/plate.
Ames test:
- Signs of toxicity : Reduced bacterial background lawn at 5000 µg/plate in all three strains.
Applicant's summary and conclusion
- Conclusions:
- With and without addition of S9 mix as the external metabolizing system, the test item was mutagenic in Salmonella typhimurium TA 100 and Escherichia coli WP2 uvrA under the experimental conditions described.
- Executive summary:
Study design
The mutagenic potential of the test item was examined in a screening assay equivalent to OECD GL 471 using Salmonella typhimurium tester strains TA 98, TA 100 and Escherichia coli WP2 uvrA. The plate incorporation test (two parallel plates per condition) with and without addition of liver S9 mix from Aroclor 1254-pretreated rats was used. The test item was dissolved in DMSO and tested at concentrations ranging from 5 - 5000 μg/plate.
Results
Precipitation of the test item on the agar plates occurred at concentrations of >= 1580 μg/plate and toxicity to bacteria at 5000 µg/plate. The treatment with the test materials used as positive controls led to a clear increase in revertant colonies, thus, showing the expected reversion properties of all strains and good metabolic activity of the S9 mix used. Following test item treatments in the absence and presence of S9, the test item was mutagenic in Salmonella typhimurium TA 100 and Escherichia coli WP2 uvrA.
Conclusion
With and without addition of S9 mix as the external metabolizing system, the test item was mutagenic in Salmonella typhimurium TA 100 and Escherichia coli WP2 uvrA.
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