Escin

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2016

Report date:

2016

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

13 April 2004

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)

Version / remarks:

April 1996

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Escin

EC Number:

229-880-6

EC Name:

Escin

Cas Number:

6805-41-0

Molecular formula:

C110H172O48

IUPAC Name:

(2S,3S,4S,5R,6R)-6-{[(3S,4S,4aR,6aR,6bS,8R,8aR,9R,10R,12aS,14aR,14bR)-9-(acetyloxy)-8-hydroxy-4,8a-bis(hydroxymethyl)-4,6a,6b,11,11,14b-hexamethyl-10-[(2-methylbutanoyl)oxy]-1,2,3,4,4a,5,6,6a,6b,7,8,8a,9,10,11,12,12a,14,14a,14b-icosahydropicen-3-yl]oxy}-4-hydroxy-3,5-bis({[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy})oxane-2-carboxylic acid; (2S,3S,4S,5R,6R)-6-{[(3S,4S,4aR,6aR,6bS,8R,8aR,9R,10R,12aS,14aR,14bR)-9-(acetyloxy)-8-hydroxy-4,8a-bis(hydroxymethyl)-4,6a,6b,11,11,14b-hexamethyl-10-{[(2E)-2-methylbut-2-enoyl]oxy}-1,2,3,4,4a,5,6,6a,6b,7,8,8a,9,10,11,12,12a,14,14a,14b-icosahydropicen-3-yl]oxy}-4-hydroxy-3,5-bis({[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy})oxane-2-carboxylic acid

Test material form:

solid: bulk

Specific details on test material used for the study:

Horse Chestnut dry hydroalcoholic extract contained 18-22% of escin

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Study design

Total exposure duration:

48 h

Test conditions

Hardness:

The reconstitute water (ISO medium) has an approximate theoretical total hardness of 249 mg/L (as CaCO3)

Test temperature:

20.1-21.1°C Experimental I and 19.8-20.3 Experimental II

pH:

7.5-8.12 Experimental I and 7.29-7.99 Experimental II

Dissolved oxygen:

Range 7.02- 8.21 mg/L Experimental I and 6.54-8.02 mg/L Experimental II

Details on test conditions:

Feeding: the test animals were not fed during the tests.

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

50 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

behaviour

Effect concentrations 3

Key result

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

behaviour

Effect concentrations 4

Key result

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Any other information on results incl. tables

Parameter (mg/L)	Time: 24 h	Time 48 h
EC50	>100	>100
NOEC	100	50
LOEC	>100	100
EC100	>100	>100

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

In this 48-hour full acute toxicity test with Daphnia magna the test item Horse chestnut dry hydroalcoholic extract 16-22% has significant toxic effect on Daphnia up to a concentration of 100 mg/L in the Experimental I. Accordingly, the 48-hour NOEC was determined to be 50mg/L and the 48-hour LOEC was determined to be 100mg/L. The 48-hour EC50 and EC100 values were determined to be >100mg/L.