3-nitro-o-xylene

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: National Guideline study with acceptable restrictions

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Unnamed

Year:

1986

Reference 2

Reference Type:

publication

Title:

A QSAR study of the toxicity of nitrobenzene derivatives towards Daphnia magna, Chlorella pyrenoidosa and Photobacterium phosphoreum.

Author:

Deneer JW, van Leeuwen CJ, Seinen W, Maas-Diepeveen JL, Hermens JLM

Year:

1989

Bibliographic source:

Aquatic Toxicology 15: 83-98

Materials and methods

Principles of method if other than guideline:

other: NEN 6502: Determination of chronic toxicity to Daphnia magna (1980), Dutch Standardization Organization, Rijswijk, The Nederlands

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

3-nitro-o-xylene

EC Number:

201-474-3

EC Name:

3-nitro-o-xylene

Cas Number:

83-41-0

Molecular formula:

C8H9NO2

IUPAC Name:

1,2-dimethyl-3-nitrobenzene

Test material form:

liquid

Details on test material:

3-Nitro-o-xylene
pure (not specified)

Sampling and analysis

Analytical monitoring:

not specified

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

- all daphnids used were <24 h old at the start of the experiment

Study design

Test type:

semi-static

Water media type:

freshwater

Total exposure duration:

21 d

Test conditions

Hardness:

200 mg/L CaCO3

Test temperature:

20 ± 0.5 °C

pH:

8.4 ± 0.1

Dissolved oxygen:

medium was saturated with air prior to use

Details on test conditions:

- test was carried out in a room that was illuminated 12 h/day
- a synthetic test medium was used (NPR 6503,1980)
- daphnids were fed with 3x10^8 (cells/L day) of Chlorella pyrenoidosa.

Results and discussion

Effect concentrations

Duration:

21 d

Dose descriptor:

LC50

Effect conc.:

3.8 mg/L

Nominal / measured:

not specified

Conc. based on:

not specified

Basis for effect:

immobilisation

Remarks on result:

other: 95% C.L. (3.6 - 4.0)

Reported statistics and error estimates:

IC50 values were calculated according to Kooyman (1981)

Any other information on results incl. tables

- Mortality in the controls never exceeded 10 %

Two other chronic effects are presented in the publications:
1. Population growth:
LRCT(rm) = Lowest rejected concentration tested that significantly (p<0.01)lowered the population growth constant(rm) after 21 days of exposure.
logLRCT(rm) = 1.61 [µmol/L] corresponds to 5.6 mg/L
2. Body length:
LRCT(length) = Lowest rejected concentration tested that significantly (p<0.01)lowered the mean length (L) of animals after 21 days of exposure.
logLRCT(length) = 1.61 [µmol/L] corresponds to 5.6 mg/L

 

The concentrations were determined using Williams´ procedure (Williams, 1971, 1972).