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Diss Factsheets
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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Principles of method if other than guideline:
- Directive 79/831 EWG Appendix V (revised edition, 1990)
- GLP compliance:
- no
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- TEST ORGANISM:
- mixed population of aquatic microorganisms of a laboratory sewage treatment plant, treating predominantly domestic sewage (Wupper area water authority, STP Odenthal, Germany)
- pretreatment: none
- concentration: 30 mg/L (dry weight) - Duration of test (contact time):
- 28 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Reference substance:
- aniline
- Remarks:
- purity: not specified
- Parameter:
- % degradation (O2 consumption)
- Value:
- 25
- Sampling time:
- 8 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 31
- Sampling time:
- 12 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 39
- Sampling time:
- 18 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 43
- Sampling time:
- 22 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 50
- Sampling time:
- 28 d
- Details on results:
- Degradation products: no
Chemcal oxygen demand (COD) of test substance: 2120 mg O2/g - Results with reference substance:
- Kinetic of control substance (in %):
2 after 8 day(s)
67 after 12 day(s)
76 after 28 day(s) - Validity criteria fulfilled:
- yes
- Remarks:
- toxicity control: negative
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The test item is not readily biodegradable. Within 28 days only 50 % of the test substance was degraded in a test conducted according to the EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)(Bayer AG, 1994).
- Executive summary:
The test item is not readily biodegradable. Within 28 days only 50 % of the test substance was degraded in a test conducted according to the EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)(Bayer AG, 1994).
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 302 C (Inherent Biodegradability: Modified MITI Test (II))
- Version / remarks:
- (based on standard operating procedures for OECD Guideline 301F (1992) and the Council Regulation (EC) No 440/2008, method C.4-D “Manometric Respirometry Test” (2008))
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Type : mixed population of aquatic microorganisms (activated sludge)
- Origin : 2 x 40 % activated sludge of an aeration tank of two different wastewater plants treating predominantly domestic sewage (Wupper area water authority, STP Odenthal and STP Cologne-Stammheim) 20 % activated sludge of aeration tanks of a sewage treatment plant treating predominantly sewage of industrial origin (STP Leverkusen Bürrig)
- Date of collections : 2009-07-21
- Concentration of inoculum (dry weight) : 100 mg/L suspended solids (ss) for all treatments with test item and toxicity control and 30 mg/L (ss) in the reference compound, respectively
CHEMICAL ANALYSIS
- COD
- Method : Determination of Chemical Oxygen Demand of solids and substances which are not water-soluble (COD).
- Calibration Standard : Potassium hydrogen phthalate
- Test apparatus : Titrator / Methrohm 730/759 - Duration of test (contact time):
- 28 d
- Details on study design:
- PRETREATMENT of the inoculum
- the three slugdes were mixed proportionally as described before
- the sludge was washed twice by adding mineral medium and centrifuging for 10 min at 2000 rpm and 20 °C and decanting off the supernatant
- 0.820 g of the wet sludge were dried in order to calculate the amount of wet sludge to achieve a concentration of activated sludge of 5 g/L (ss)
- the calculated amount of sludge was first dissolved in mineral medium and then filled up to a defined end volume
- the inoculum was stored at room temperature under continuous shaking with aeration until use for one day
PRETREATMENTof the test item
-7.5 mg of the test item were weighed out on aluminium foil. Both were added into the test flasks, which had already been filled with 200 mL of mineral medium. Afterwards the flask content was made up to 250 mL with mineral medium containing inoculum to give a test concentration of 30 mg test item/L.
- For the toxicity control 7.5 mg were weighed out to give a test concentration of 30 mg test item/L.
PRETREATMENT of the reference compound
- Reference compound name : sodium benzoate (Fluka-BioChemika)
- 25 mg of the reference compound were weighed out on aluminium foil. Both were added into the test flasks, which had already been filled with 200 mL of mineral medium. Afterwards the flasks were made up to 250 mL with mineral medium containing inoculum to give a test concentration of 100 mg reference compound/L. For the toxicity control 7.5 mg were weighed out to give a test concentration of 30 mg reference compound/L.
EXPOSURE CONDITIONS
- Test volume : 250 mL
- Test apparatus : OxiTop System (WTW)
- Mixing : 1 magnetic stirrer per test vessel
- Incubation time : 28 days
- Incubation temperature : 25 ± 1 °C - Reference substance:
- benzoic acid, sodium salt
- Remarks:
- - Purity : 100.1 % - Batch-no. : 1373379
- Parameter:
- % degradation (O2 consumption)
- Value:
- 73
- Sampling time:
- 28 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 53
- Sampling time:
- 7 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 68
- Sampling time:
- 14 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 72
- Sampling time:
- 21 d
- Results with reference substance:
- The reference compound sodium benzoate showed 78 % degradation after 7 days and 80 % degradation after 14 days.
- Validity criteria fulfilled:
- yes
- Remarks:
- The percent degradation of reference compound sodium benzoate reached the level of ≥ 40 % after 7 days and ≥ 65 % after 14 days. No toxicity of the test item was observed in the toxicity control.
- Interpretation of results:
- inherently biodegradable
- Conclusions:
- Within 28 days a degradation of 73 % was determined for the test item according to the OECD Guideline 302 C. Therefore the test item is considered to be "Inherently Biodegradable" (Currenta, 2009).
- Executive summary:
Within 28 days a degradation of 73 % was determined for the test item according to the OECD Guideline 302 C. Therefore the test item is considered to be "Inherently Biodegradable" (Currenta, 2009).
Referenceopen allclose all
Description of key information
A test on ready biodegradability showed that 50 % of the substance is degraded within 28 days. These results show that the substance is not readily biodegradable, but additionally provided evidence from an inherent test show that the substance can be degraded in sewage treatment plants. The substance is not readily biodegradable but inherent biodegradable (Bayer AG, 1994; Currenta, 2009).
Key value for chemical safety assessment
- Biodegradation in water:
- inherently biodegradable, fulfilling specific criteria
- Type of water:
- freshwater
Additional information
The ready biodegradability of the substance was determined according to the EU method C.4 -D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test) with the following result (Bayer AG, 1994): 50 % degradation after 28 days.
The inherent biodegradability was determined according to the OECD Guideline 302 C (Inherent Biodegradability: Modified MITI Test (II)) and results in 73 % degradation within 28 days (Currenta, 2009).
Based on these results, the substance is considered to be "Not Readily Biodegradable", but "Inherently Biodegradable" .
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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