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EC number: 275-139-5 | CAS number: 71032-99-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- Range finding test: 15th February, 1991. Main test: 21st February, 1991.
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Justification for type of information:
- The test substance is the acid form of the substance under registration; justification for Read Across is detailed in the IUCLID section 13.
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- not specified
- Details on test solutions:
- Test item has been direct dispersion in water.
- Test organisms (species):
- activated sludge
- Details on inoculum:
- - Source: the aeration Stage of Severn Trent PLC Sewage Treatment Plant, Belper, Derbyshire.
- Preparation: the sample was maintained at 21 °C with continuous aeration and used on the day of collection. The pH was buffered to pH 7.0 with sodium hydroxide solution and the suspended solids level adjusted to 3.9 g/l prior to use. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Nominal and measured concentrations:
- Range-finding test: 1.0, 10, 100, 1000 mg/l (nominal)
Main test: 1000 mg/l (nominal) - Details on test conditions:
- TEST SYSTEM
- No. of vessels per concentration: one replicate per concentration in the range-finding test; three replicated in the main test.
- No. of vessels per control: two replicates - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- MAIN TEST
Only slight effects were recorded: the percentage of the respiration inhibition ranges from 8 to 17.
Variation between the controls: ± 2 %
RANGE-FINDING TEST
Variation between the controls: 0 % - Results with reference substance (positive control):
- EC50 (3h): 11.5 mg/l
- Conclusions:
- EC50 (3h) > 1000 mg/l (nominal)
- Executive summary:
Activated sludge respiration inhibition test has been performed on test item. A range-finding investigation was conducted at the nominal concentrations of 1.0, 10, 100 and 1000 mg/l. The main test was conducted at the nominal concentration of 1000 mg/l; three replicates were assayed. Blank control and reference item, 3,5-dichlorophenol, have also been tested.
Only slight effects were recorded: the percentage of the respiration inhibition ranges from 8 to 17. The EC50 after 3 hours of contact time has been indicate as higher than 1000 mg/l.
Conclusion
EC50 (3h) > 1000 mg/l (nominal)
Reference
Main test
Concentration (mg/l ) |
O2 consumption rates mg/O2/l/min |
% inhibition |
Control | 0.61 | - |
Control | 0.59 | - |
Test item 1000 | 0.52 | 13 |
Test item 1000 | 0.5 | 17 |
Test item 1000 | 0.55 | 8 |
3,5-dichlorophenol 3.2 | 0.61 | <2> |
3,5-dichlorophenol 32 | 0.06 | 90 |
<increase>
Range-finding test
Concentration (mg/l ) |
O2 consumption rates mg/O2/l/min |
% inhibition |
Control | 0.41 | - |
Control | 0.41 | - |
Test item 1.0 | 0.42 | <2> |
Test item 10 | 0.35 | 15 |
Test item 100 | 0.39 | 5 |
Test item 1000 | 0.34 | 17 |
3,5-dichlorophenol 3.2 | 0.38 | 7 |
3,5-dichlorophenol 32 | 0.01 | 98 |
<increase>
Description of key information
Not harmful/toxic to microorganisms. EC50 (3h) > 1000 mg/l (nominal)
Key value for chemical safety assessment
Additional information
There are no information about the toxicity potential to microorganisms of Basic Yellow 094, thus the available information on the structural analogue Similar Substance 01 have been taken into consideration. Similar Substance 01 is the acid form of Basic Yellow 094; the read across approach can be considered as appropriate and suitable to assess the property under investigation (details about the approach are reported into the IUCLID section 13).
Activated sludge respiration inhibition test has been performed on Similar Substane 01. On the basis of the outcomes of the range-finding investigation, the main test was conducted at the nominal concentration of 1000 mg/l. Only slight effects were recorded: the percentage of the respiration inhibition range from 8 to 17. The EC50 after 3 hours of contact time has been indicate as higher than 1000 mg/l.
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