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Diss Factsheets
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EC number: 200-735-9 | CAS number: 70-47-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Study according to OECD Test Guideline 439, GLP, in the EPISKIN-SM™ model, the mean relative tissue viability (% negative control) was > 50% (98.8%) after 15 min treatment and 42 h post-incubation.
Study according to OECD Test Guideline 492, GLP, three-dimensional reconstructed human cornea-like epithelium (RhCE) EpiOcular™ (MatTek), the mean relative tissue viability (% negative control) was > 60% (79.2%).
Respiratory irritation: No study available, inhalation is not the expected route of exposure.
Key value for chemical safety assessment
Skin irritation / corrosion
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not irritating)
Eye irritation
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not irritating)
Respiratory irritation
Endpoint conclusion
- Endpoint conclusion:
- no study available
Additional information
Skin irritation (in vitro):
In an in vitro skin irritation study according to OECD Test Guideline 439, GLP compliant, the EPISKIN-SM™ reconstructed human epidermis model (SkinEthic) which consists of normal human epidermal keratinocytes (NHEK) was exposed to L-asparagine for 15 min and subsequently incubated for 42h. After the incubation period the cytotoxicity was measured using the MTT test system. Cytotoxicity is expressed as the reduction of mitochondrial dehydrogenase activity measured by formazan production from MTT after the incubation period and compared to those of the concurrent negative controls. The test is considered valid if the mean relative tissue viability (% negative control) is > 50%. The mean relative tissue viability (% negative control) was 98.8% after 15 min treatment and 42 h post-incubation. Thus, L-asparagine is considered to be not irritating to the skin.
Eye irritation (in vitro):
In an in vitro eye irritation study according to OECD Test Guideline 492, GLP compliant, the test uses the three-dimensional reconstructed human cornea-like epithelium (RhCE) EpiOcular™ (MatTek) which was incubated with the test item for 6h and subsequently incubated without test item (post-incubation period). After the incubation period the cytotoxicity was measured using the MTT test system. Cytotoxicity is expressed as the reduction of mitochondrial dehydrogenase activity measured by formazan production from MTT after the incubation period and compared to those of the concurrent negative controls. The test is considered to be valid if the mean relative tissue viability (% negative control) is >60%. The mean relative tissue viability was 79.2%. Thus, L-asparagine is considered to be not irritating to the eyes.
Respiratory irritation:
No data on the respiratory irritation of L-asparagine are available.
There are no data gaps for the endpoint irritation/corrosion. No human information is available for this endpoint. However, there is no reason to believe that these results would not be applicable to humans.
Justification for classification or non-classification
Skin irritation was determined with the EPISKIN-SM™ reconstructed human epidermis model (SkinEthic) which consists of normal human epidermal keratinocytes (NHEK) and therefore represents in vitro the target organ of the species of interest and closely mimics the biochemical and physiological properties of the upper parts of the human skin, i.e. the epidermis.
This test method is able to detect chemicals that cause skin irritation, i.e. produce reversible damage to the skin and allows for hazard identification in accordance with UN GHS “Category 2”. Depending on the regulatory framework it can also be used to identify non-classified chemicals. Similarily, eye irritation was determined using the three-dimensional reconstructed human cornea-like epithelium (RhCE) EpiOcular™ (MatTek), which is recommended to identify chemicals that do not require classification for eye irritation or serious eye damage according to UN GHS (UN GHS “No Category”) without further testing within a tiered testing strategy from those requiring classification and labelling. Thus, both in vitro systems are applicable and reliable for testing of an irritation potential.
Based on the available, relvant and adequate data the substance does not need to be classified according to Regulation (EC) No 1272/2008 (CLP) and the Globally Harmonized System for Classification and Labeling of Chemicals (GHS) with respect to irritation/corrosion.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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