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EC number: 272-447-1 | CAS number: 68845-00-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 302 C (Inherent Biodegradability: Modified MITI Test (II))
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Commercial name : BOISIRIS
Chemical nature : 2-ethoxy-9-methylene-2,6,6-trimethylbicyclo[3.3.1]nonane
Empirical formula : C15H26O
Molecular weight : 222.3 g/mol
CAS Number : 68845-00-1
Aspect : colourless to pale yellow liquid
Lot No. : 263382 - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- Fresh activated sludge from a biological waste water treatment plant treating predominantly domestic sewage (City of Geneva, Aïre) was used.
The sludge is collected in the morning, washed three times in the mineral medium (by centrifuging at 1000 g for 10 minutes, discarding the supernatant and resuspending in mineral medium) and kept aerobic until being used on the same day. - Duration of test (contact time):
- 31 d
- Initial conc.:
- 30 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Reference substance:
- benzoic acid, sodium salt
- Remarks:
- The reference substance is used to test the activity of the inoculum used in a series of parallel studies. The reference substance concentration is 100 mg/l and the inoculum concentration is 100 mg/l (dry weight).
- Test performance:
- Oxygen uptakes, as recorded by the BI-1000, are corrected:
- by deducting the basic oxygen uptake of sludge (average of flasks 1/5 and 1/6)
- proportionally to account for the differences between actual and nominal concentrations of test substances.
Averages of identical flasks were used to obtain the different results and curves that can be found in Appendixes 1, 2, 3 and 4.
Degradation of sodium benzoate is 68 % after 7 days and 94 % after 14 days, widely in excess of the validity criterium: the activity of the inoculum is thus verified and the test is considered as valid. - Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 31 d
- Details on results:
- BOISIRIS undergoes no biodegradation after 31 days in the test conditions.
Thus, BOISIRIS should be regarded as not inherently biodegradable according to this test.
However, inhibition of the inoculum was observed. This is clearly seen in the biodegradation time plot, where negative levels of biodegradation are observed on most days and in particular during the first 7 days (see illustration section). Thus, the influence of toxicity on the result of this test cannot be ruled out. - Validity criteria fulfilled:
- yes
- Interpretation of results:
- not inherently biodegradable
- Conclusions:
- The Inherent Biodegradability of BOISIRIS was determined by the Manometric Respirometry Test according to the OECD Guidelines for Testing of Chemicals, Method No. 302 C.
BOISIRIS undergoes no biodegradation after 31 days in the test conditions.
Thus, BOISIRIS should be regarded as not inherently biodegradable according to this test.
At the test concentration, Boisiris did inhibit the intrinsic respiration of the inoculum. This is clearly seen in the biodegradation time plot, where negative levels of biodegradation are observed on most days and in particular during the first 7 days. Thus, the influence of toxicity on the result from this test cannot be ruled out for this substance. - Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Qualifier:
- according to guideline
- Guideline:
- other: Revised Introduction to the OECD Guidelines for Testing of Chemicals, Section 3, Part 1, 2006
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Qualifier:
- according to guideline
- Guideline:
- other: ECHA Guidance for the implementation of REACH, Guidance on information requirements and chemical safety assessment, Chapter R.7b: Endpoint specific guidance, November 2014 (version 2.0)
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 835.3110 (Ready Biodegradability)
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Commercial name : Boisiris
Givaudan Code No. : 2786903
Chemical nature : 2-Ethoxy-9-methylene-2,6,6-trimethylbicyclo(3.3.1)-nonane
Empirical formula : C15H26O
Molecular weight : 222.37 g/mol
CAS No. : 68845-00-1
Aspect : Colourless to pale yellow liquid
Lot No. : VE00383856
Expiration date : August 06, 2017 - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- Fresh activated sludge from a biological waste water treatment plant treating predominantly domestic sewage (Bois-de-Bay, Satigny, Switzerland) was used.
The sludge is collected in the morning, washed three times in the mineral medium (by centrifuging at 1000 g for 10 minutes, discarding the supernatant and resuspending in mineral medium) and kept aerobic until being used on the same day. - Duration of test (contact time):
- 42 d
- Initial conc.:
- 30 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Reference substance:
- benzoic acid, sodium salt
- Test performance:
- Oxygen uptakes, as read on the Oxitop controller, are corrected to account for the small differences between actual and nominal concentrations of test and reference substances.
Degradation of sodium benzoate exceeded 40% after 7 days and 65% after 14 days: the activity of the inoculum was thus verified (validity criterion).
The repeatability validity criterion (not more than 20% difference between replicates) is fulfilled. Therefore, the test is considered valid. - Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 1
- Sampling time:
- 28 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 4
- Sampling time:
- 42 d
- Details on results:
- Boisiris undergoes 1% biodegradation after 28 days (4% after 42 days) in the test conditions.
At the test concentration Boisiris did inhibit the intrinsic respiration of the inoculum by 22.8% on day 1 of the test. Slight toxicity towards the inoculum at test concentration can therefore not be excluded.
Boisiris should be regarded as not readily biodegradable according to this test. - Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The Ready Biodegradability of Boisiris was determined by the Manometric Respirometry Test.
Boisiris undergoes 1% biodegradation after 28 days (4% after 42 days) in the test conditions.
At the test concentration Boisiris did inhibit the intrinsic respiration of the inoculum by 22.8% on day 1 of the test. Slight toxicity towards the inoculum at test concentration can therefore not be excluded.
Boisiris should be regarded as not readily biodegradable according to this test.
Referenceopen allclose all
Description of key information
The substance is neither readily or inherently biodegradable according to the screening data available. The levels of biodegradation observed are <= 4%. Thus the conclusion is that under these test conditions no biodegradation (based on oxygen consumption) was observed.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
The ready biodegradability of Boisiris has been investigated in an OECD 301F screening test. The level of biodegradation based on BOD (biological oxygen demand) was 1% after 28 days (4% after 42 days). Thus Boisiris is regarded as not readily biodegradable (or inherently biodegradable) according to this test. At the test concentration of 30 mg/L, Boisiris did inhibit the intrinsic respiration of the inoculum by 22.8% on day 1 of the test. Thus toxicity towards the inoculum at the test concentration can not be excluded.
The inherent biodegradability was assessed according to OECD Guideline 302C. The test item concentration in this study was also 30mg/L. The main difference between this inherent test and the aforementioned ready test is the higher concentration of inoculum employed (100 mg/L versus 30 mg/L). Boisiris underwent no biodegradation after 31 days and thus should be regarded as not inherently biodegradable according to this test. However, inhibition of the inoculum was again observed. This is clearly seen in the biodegradation time plots, where negative levels of biodegradation are observed on most days and in particular during the first 7 days.
The registrant considers that the influence of toxicity on the results of these two screening tests cannot be ruled out for this substance. Furthermore, the 30mg/L test concentration employed in the two studies is significantly higher than the water solubility of Boisiris, which is only 4mg/L. Thus, bioavailability is another potential issue for the screening data available.
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