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EC number: 203-467-0 | CAS number: 107-14-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 995
Materials and methods
- Principles of method if other than guideline:
- The Ames fluctuation test, an in vitro test detecting point mutation on Salmonella typhimurium. This assay is the modification, in liquid medium, of the widely used Ames/Salmonella assay. Thanks to its better sensitivity, the fluctuation test is very well suited to the search for mutagenicity in water samples (Wilcox and Denny, 1985; Monarca et al., 1985).
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Chloroacetonitrile
- EC Number:
- 203-467-0
- EC Name:
- Chloroacetonitrile
- Cas Number:
- 107-14-2
- Molecular formula:
- C2H2ClN
- IUPAC Name:
- 2-chloroacetonitrile
- Test material form:
- liquid
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 100
- Details on mammalian cell type (if applicable):
- Source of cells: B.N. Ames (University of California, Berkeley, USA)
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 (rat)
- Test concentrations with justification for top dose:
- 3-300 µg/ml without metabolic activation, 1-1000 µg/ml with metabolic activation
- Vehicle / solvent:
- DMSO
Controls
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Remarks:
- 1 ng/ml 4-NQO without the metabolic system and 60 µg/ml CPA with metabolic activation
- Details on test system and experimental conditions:
- The Ames-fluctuation test was performed as described by Hubbard et al. (1984). This assay is a modification of the Ames test: the compound under study is exposed to bacteria in a liquid medium in many replicate cultures (96-well microplate) instead of the agar plate used in the Ames assay.
After the 3-day incubation, bromothymol blue (600 µg/ml) was added. Positive wells (containing prototrophic mutants) turned yellow whereas negative wells remained green. For each experiment, spontaneous reversion in response to the solvent used (DMSO) was included. Compounds were tested at least twice (two independent assays) using 3 experimental points for each concentration. - Rationale for test conditions:
- Strain TA100 was used during all the experiment because it is considered as the most sensitive strain to water mutagens and particularly to chlorinated by-products (Loper, 1980; Forster et al., 1983; Harrington et al., 1983; Vartiainen and Liimatainen, 1986; Fielding and Horth, 1987; Meier, 1988).
- Evaluation criteria:
- A chemical is considered as toxic when it produces a significant decrease in the number of positive wells compared to the solvent control.
A compound is considered as mutagenic:
(1) if it induces a statistically significant increase in the number of positive wells compared to the solvent control,
(2) if a dose-effect relationship is noticeable and
(3) if the result is reproducible. - Statistics:
- The statistical significance of the results was assessed with the X2 test: p < 0.05 for X2 > 3.84 and p < 0.01 for X2 > 6.63 (Green et al., 1976).
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- positive
- Remarks:
- 30 µg/ml (in the presence of S9-mix only)
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- 300 µg/ml (threshold toxic concentration)
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.