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EC number: 252-478-7 | CAS number: 35274-05-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- (Q)SAR
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Calculation of the LC50 of Ceraphyl 41 based on the individual QSAR data for the C12-C15 lactates.
- Justification for type of information:
- 1. SOFTWARE: ECOSAR Program (v1.10)
2. SMILES OR OTHER IDENTIFIERS USED AS INPUT FOR THE MODEL: O=C(OCCCCCCCCCCCCCCCC)C(O)C
3. SCIENTIFIC VALIDITY OF THE (Q)SAR MODEL
- Defined endpoint: LC50
----------------------------------------------------------------------------------------------------
Class Specific LogKow Cut-Offs
----------------------------------------------------------------------------------------------------
If the log Kow of the chemical is greater than the endpoint specific cut-offs
presented below, then no effects at saturation are expected for those endpoints.
Esters:
----------------------------------------------------------------------------------------------------
Maximum LogKow: 5.0 (Fish 96-hr LC50; Daphnid LC50, Mysid LC50)
Maximum LogKow: 6.0 (Fish 14-day LC50; Earthworm LC50)
Maximum LogKow: 6.4 (Green Algae EC50)
Maximum LogKow: 8.0 (ChV)
Baseline Toxicity SAR Limitations:
----------------------------------------------------------------------------------------------------
Maximum LogKow: 5.0 (Fish 96-hr LC50; Daphnid LC50)
Maximum LogKow: 6.4 (Green Algae EC50)
Maximum LogKow: 8.0 (ChV)
----------------------------------------------------------------------------------------------------
4. ADEQUACY OF THE RESULT
- Data can be used to determine the classification category and M-factor. - Principles of method if other than guideline:
- ECOSAR v1.1 Class-specific Estimations based on Esters or Baseline toxicity.
- GLP compliance:
- no
- Test organisms (species):
- other: Fresh water algae
- Water media type:
- freshwater
- Total exposure duration:
- 96 h
- Key result
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Remarks:
- Freshwater
- Effect conc.:
- 0.019 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Remarks:
- Marine water
- Effect conc.:
- 0.008 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Test Item Name CERAPHYL® 41
CAS Number 93925-36-1
Batch/Lot Number 0001749388
Analysed Purity 100 % (Refer Certificate of Analysis in APPENDIX 7)
Manufactured by Ashland Inc.
Supplied to JRF by Ashland Inc.
Date of Manufacture June 18, 2014
Date of Expiry June 18, 2016
Appearance: Clear, water white to straw colored liquid
Storage Temperature: Ambient
Storage Container: In original container as supplied by the Sponsor - Analytical monitoring:
- yes
- Details on sampling:
- The test solutions were analysed for concentration of dedocyl alcohol using the validated analytical method. Quantitative analysis of dodecanol, tridecanol, tetradecanol, dodecyl lactate, tridecyl lactate, tetradecyl lactate, and pentadecyl lactate was done in 100% WAF of CERAPHYL® 41 by area % normalization. Two replicates each of 100.5 mL were centrifuged at 2000 rpm for 10 minutes. Collected sample were centrifuged in order to remove algal cell. Samples were sent for test concentration analysis at 0 h.. Same procedure was followed for sample collection at 24 h, 48 h and 72 h.
- Details on test solutions:
- Quantity of 202.6 mg CERAPHYL® 41 were weighed in two replicates, and made up to 2000 mL with sterile distilled water in two different glass bottles (stock A and B) and kept for continuous stirring using magnetic stirrer for approximate 24 h at room temperature. After stirring, test solutions were kept for re-equilibrium for approximate 24 h. After phase separation, test solutions were collected from lower portion by “L” shaped glass tube without disturbing the phase. Volume of 1200 mL was collected from each bottle and mixed together (stock C). To prepared the concentration of 100% WAF prepared at 100 mg CERAPHYL® 41/L and OECD medium preparation, after sample collection, total 31.2 mL of OECD stock solutions (24 mL of stock solution I, 2.4 mL of stock solution II, 2.4 mL of stock solution III, and 2.4 mL of stock solution IV) were added to the collected sample for preparation of algal medium and used for the treatment.
The initial cell concentration of the alga stock culture was determined as 6138 cells/mL by using a haemocytometer and microscope. Each replicate was inoculated with 0.5 mL of alga culture to obtain the required cell density. - Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- Strain Number : ATCC 22662
Origin and Source of Culture: American Type Culture Collection, 10801, University of Boulevard, Manassas, Virginia, 20110-2209, USA
Subculture Maintained at : Jai Research Foundation
Date of Receipt (at JRF) : May 13, 2010
Date of Last Subculture : January 19, 2016
Method of Cultivation : Static condition - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Test temperature:
- 22 - 24 °C
- pH:
- 8.0 - 8.2
- Nominal and measured concentrations:
- 100% WAF prepared at 100 mg CERAPHYL® 41/L corresponding with an initially measured concentration of 1.8 mg/L
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1.8 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (total fraction)
- Basis for effect:
- growth rate
- Remarks on result:
- other: Concentration refers to the nominal loading.
Referenceopen allclose all
--------------------------------------
ECOSAR v1.1 Class-specific Estimations
--------------------------------------
Esters
Predicted
ECOSAR Class Organism Duration End Pt mg/L (ppm)
=========================== ================== ======== ====== ==========
Esters : Fish 96-hr LC50 0.076
Esters : Daphnid 48-hr LC50 0.096
Esters : Green Algae 96-hr EC50 0.019
Esters : Fish ChV 0.002
Esters : Daphnid ChV 0.017
Esters : Green Algae ChV 0.023
Esters : Fish (SW) 96-hr LC50 0.087
Esters : Mysid 96-hr LC50 0.008
Esters : Fish (SW) ChV 0.029
Esters : Mysid (SW) ChV 4.39e-005
Esters : Earthworm 14-day LC50 125.895 *
=========================== ================== ======== ====== ==========
Neutral Organic SAR : Fish 96-hr LC50 0.016
(Baseline Toxicity) : Daphnid 48-hr LC50 0.020
: Green Algae 96-hr EC50 0.019
: Fish ChV 0.003
: Daphnid ChV 0.004
: Green Algae ChV 0.042
Note: * = asterisk designates: Chemical may not be soluble enough to
measure this predicted effect. If the effect level exceeds the
water solubility by 10X, typically no effects at saturation (NES)
are reported.
------------------------------
Class Specific LogKow Cut-Offs
------------------------------
If the log Kow of the chemical is greater than the endpoint specific cut-offs
presented below, then no effects at saturation are expected for those endpoints.
Esters:
------
Maximum LogKow: 5.0 (Fish 96-hr LC50; Daphnid LC50, Mysid LC50)
Maximum LogKow: 6.0 (Fish 14-day LC50; Earthworm LC50)
Maximum LogKow: 6.4 (Green Algae EC50)
Maximum LogKow: 8.0 (ChV)
Baseline Toxicity SAR Limitations:
---------------------------------
Maximum LogKow: 5.0 (Fish 96-hr LC50; Daphnid LC50)
Maximum LogKow: 6.4 (Green Algae EC50)
Maximum LogKow: 8.0 (ChV)
Description of key information
Estimation of the endpoint value for acute toxicity to invertebrates was based on a GLP study with C12-15 alkyl lactates, supported by EC50 values estimated by ECOSAR v. 1.1 (QSAR). C12-15 alkyl lactates were tested in a static 72-h test with Pseudokirchneriella subcapitata as test organism. The EC50 of C12-15 alkyl lactates was above its solubility limit, i.e. a TWA of 1.8 mg/L. Based on ECOSAR v1.1, EC50 of hexadecyl lactate for fresh water algae = 0.019 mg/L, and for sea water algae = 0.008 mg/L. These values were based on the Ester class. Hence, these key values reflect a worse case scenario.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 0.019 mg/L
- EC50 for marine water algae:
- 0.008 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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