cis-3a,4,9,9a-tetrahydro-2,2-dimethylnaphtho[2,3-d]-1,3-dioxol-5-ol

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

14/04/2016-27/06/2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2016

Report date:

2016

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)

Version / remarks:

OECD Guideline for Testing of Chemicals, Section 2, No. 201: "Freshwater Alga and Cyanobacteria, Growth Inhibition Test", adopted March 23, 2006, corrected July 28, 2011

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.3 (Algal Inhibition test)

Version / remarks:

Commission Regulation (EC) No 761/2009, Annex, Part C, C.3.: "Freshwater Algae and Cyanobacteria, Growth Inhibition Test", Official Journal of the European Union (EN), dated August 24, 2009

Principles of method if other than guideline:

SANCO/3029/99 rev.4 11/07/00: Residues: Guidance for generating and reporting methods of analysis in support of preregistration data requirements for Annex II (part A; Section 4) and
Annex III (part A; Section 5) of directive 91/414

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

cis-3a,4,9,9a-tetrahydro-2,2-dimethylnaphtho[2,3-d]-1,3-dioxol-5-ol

EC Number:

257-722-6

EC Name:

cis-3a,4,9,9a-tetrahydro-2,2-dimethylnaphtho[2,3-d]-1,3-dioxol-5-ol

Cas Number:

52187-19-6

Molecular formula:

C13H16O3

IUPAC Name:

2,2-dimethyl-3a,4,9,9a-tetrahydronaphtho[2,3-d][1,3]dioxol-5-ol

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 101%, according to certificate of analysis
- Sample storage conditions before analysis: At 20+/- 5°C in the dark

Test solutions

Vehicle:

not specified

Test organisms

Test organisms (species):

Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)

Details on test organisms:

TEST ORGANISM
- Common name: Pseudokirchneriella subcapitata (KORSHIKOV)
- Strain: No. 61.81 SAG
- Source (laboratory, culture collection): supplied by the „Sammlung von Algenkulturen, Albrecht-von-Haller-Institut für Pflanzenwissenschaften, Universität Göttingen", 37073 Göttingen, Germany.
- Method of cultivation: The algae were cultivated in the laboratories of ibacon under standardised conditions according to the test guidelines.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Hardness:

Calculated water hardness of the test water: 0.24 mmol/L (= 24 mg/L) as CaCO3.

Test temperature:

22 - 23°C

pH:

pH values at test start 7.8 to 8.0, at the end of the test 8.6 to 9.5

Nominal and measured concentrations:

At the start of the test 106% of the nominal test concentrations were found (average of all test concentrations). After 72 hours test duration, 62% of the nominal value was determined (average of all test concentrations). Therefore, all reported results refer to geometric mean concentrations, since the test item concentrations were not within ± 20% of the nominal concentrations during the test.

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 71.8 mg/L

Nominal / measured:

meas. (not specified)

Effect concentrations 2

Key result

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

8.31 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The influence of CIS-TRIOL ACETONIDE on the growth of the freshwater green algae Pseudokirchneriella subcapitata was assessed in a static concentration-response test. The 72-hour EyC50 was calculated to be > 71.8 mg test item/L and the 72-hour ErC50 value was calculated to be > 71.8 mg test item/L. The 72-hour NOEyC was determined to be 8.31 mg test item/L and the associated 72-hour LOEyC was 26.1 mg test item/L. The 72-hour NOErC was determined to be 8.31 mg test item/L and the associated 72-hour LOErC was 26.1 mg test item/L. All values refer to geometric mean measured concentrations.