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EC number: 228-995-9 | CAS number: 6388-26-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- From March 29 to April 26, 2007
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
- Version / remarks:
- 1992
- GLP compliance:
- no
- Remarks:
- according to ISO/IEC 17025
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: ARA Werdhölzli, CH-8048 Zürich, 28 March 2007, 11.00 p.m.
- Concentration of sludge: 0.2 g/l dry matter in the final mixture
- Storage length: used immediately after sampling from the treatment plant without adaptation
- Preparation of inoculum for exposure: washed twice with tap water - Duration of test (contact time):
- 28 d
- Initial conc.:
- 50 mg/L
- Based on:
- DOC
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- TEST CONDITIONS
- Composition of medium: mineral salts medium prepared with double distilled water (conductivity < 1.5 µD/cm, DOC < 0.3 mg/l)
- Test temperature: 22 ± 0.5 °C
- Aeration of dilution water: yes, with synthetic CO2-free air
- pH: 6.5-8.0, adjusted with NaOH or HCl, if necessary
- Oxygen concentration: > 6 mg/l
TEST SYSTEM
- Number of culture flasks/concentration: 2 for test substance
- Test performed in closed vessels: 1200 ml closed glass bottle
- Details of trap for CO2 and volatile organics if used: gas absorption bottles filled with NaOH
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes, 2 replicates
- Positive control: yes, 1 replicate - Reference substance:
- diethylene glycol
- Remarks:
- 49.7 mg/l as DOC
- Parameter:
- % degradation (DOC removal)
- Value:
- 30
- Sampling time:
- 28 d
- Remarks on result:
- other: adsoprtion after 3 h is 0 %
- Details on results:
- Based on DOC determinations, test substance reached a biodegradation of 30 % after 28 days.
The main degradation process occurred during the first 12 days, when 30 % of the initial DOC was removed. Maximum biodegradation was attained after 12 days of incubation. No significant adaption period was observed. No siginificant elimination due to adsorption to the activated sludge, on the glass surface or other physico-chemical processes was found as determined by means of DOC measurements 3 h after the start of the test.
Biodegradation of test substance based on CO2 evolution and calculated as ThCO2 reached 12 % after 28 days of incubation, showing that only part of the organic carbon of the test material was transformed into CO2. - Results with reference substance:
- Diethylenglycole showed 99 % biodegradation as DOC removal after 14 days of incubation, thus confirming suitability of inoculum and test conditions.
The extent of mineralization of diethylenglycole was 96 % based on ThCO2. - Validity criteria fulfilled:
- yes
- Interpretation of results:
- inherently biodegradable, fulfilling specific criteria
- Conclusions:
- Test substance resulted as inherently biodegradable, based on percentage of biodegradation in terms of DOC removal above 20 % after 28 days of contact time.
- Executive summary:
Method
Inherent biodegradability was determined in a 28 days Zahn-Wellens assay according to OECD guideline 302B. Test substance was tested in concentration of 50 mg/l DOC. Diethylenglycol was used as reference substance in a concentration of 49.7 mg/l as DOC.
Additionally the degree of biodegradation was also estimated as % of ThCO2.
Results
After 28 days, biodegradation based on DOC removal and corrected by blank control was 30 % as average of 2 tests running in parallel. Adsorption after 3 hours resulted as 0 %.
Biodegradation based on CO2 evolution and calculated as % ThCO2 was 12 % after 28 days.
Biodegradation of reference substance was 99 % as DOC removal and 96 % as ThCO2 evolution within the experimental period.
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- From January 5 to February 2, 1994
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
- Version / remarks:
- 1981
- Deviations:
- yes
- Remarks:
- sludge concentration in test was 1040 mg/l instead of 200-1000 mg/l
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: sludge
- Details on inoculum:
- - Source of inoculum: biological sewage treatment plant (ARA-Therwil) collected on 04.01.94
- Concentration of sludge: 1040 mg/l - Duration of test (contact time):
- 28 d
- Initial conc.:
- 150 mg/L
- Based on:
- DOC
- Remarks:
- nominal
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- TEST CONDITIONS
- Test temperature: 22 ± 3 °C
- Lighting: indirect light
TEST SYSTEM
- Number of culture flasks: 2
- Measuring equipment: Shimadzu TOC analyzer
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes - Reference substance:
- diethylene glycol
- Parameter:
- % degradation (DOC removal)
- Value:
- 19.5
- Sampling time:
- 28 d
- Remarks on result:
- other: adsorption after 3 h: 1 %
- Results with reference substance:
- Bioelimination, corrected by blank control a measured as DOC (mg/l) is 98.8 % after 16 days.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not inherently biodegradable
- Conclusions:
- Test substance resulted as non inherently biodegradable, based on degradation percentage below 20 % after 28 days.
- Executive summary:
Method
Inherent biodegradability was determined in a 28 days Zahn-Wellens assay according to OECD guideline 302B. Test substance was tested in concentration of 150 mg/l DOC. Diethylenglycol was used as reference substance in a concentration of 150 mg/l.
Results
After 28 days, bioelimination corrected by blank control was 19.5 % as average of 2 tests running in parallel. Adsorption after 3 hours resulted to as 1 %.
Biodegradation of reference substance after 16 days was 98.8 %.
Referenceopen allclose all
DOC concentrations of test suspension, inoculum blank and procedure control and calculation of degradation data
day | inoculum blank* | reference substance* | substance* | ||||
DOC mg/l | DOC (mg/l) | corrected DOC (mg/l) | reduction % | DOC (mg/l) | corrected DOC (mg/l) | reduction % | |
0 | 0.6 | 49.6 | 49.0 | - | 46.8 | 46.2 | - |
0.125 (3 h) | 1.1 | 50.5 | 49.4 | 0 | 44.8 | 43.7 | 0 |
1 | 1.3 | 45.2 | 43.9 | 11 | 38.5 | 37.2 | 15 |
4 | 2.1 | 18.9 | 16.9 | 66 | 35.7 | 33.7 | 23 |
7 | 2.0 | 2.1 | 0.1 | 100 | 35.0 | 33.0 | 25 |
12 | 1.6 | 2.3 | 0.7 | 99 | 32.2 | 30.6 | 30 |
14 | 1.8 | 2.3 | 0.5 | 99 | 32.9 | 31.1 | 29 |
18 | 1.8 | 2.1 | 0.3 | 99 | 33.5 | 31.8 | 27 |
21 | 1.9 | 2.3 | 0.3 | 99 | 33.5 | 31.6 | 28 |
25 | 1.8 | 2.5 | 0.7 | 99 | 32.2 | 30.4 | 31 |
27 | 1.6 | 2.2 | 0.7 | 99 | 32.6 | 31.0 | 29 |
28 | 1.6 | 2.2 | 0.6 | 99 | 32.2 | 30.6 | 30 |
*mean of 2 replicates
IC concentrations, calculated from the concentrations in the gas absorption bottles, of test suspension, inoculum blank and procedure control and corresponding degradation data
time (days) | test suspension IC (mg/l) | procedure control IC (mg/l) | inoculum blank IC (mg/l) | biodegradation test suspension (% ThCO2) | biodegradation procedure control (% ThCO2) |
0 | n.d. | n.d. | n.d. | - | - |
7 | 19.1 | 57.6 | 19.3 | 0 | 77 |
14 | 29.4 | 71.2 | 27.2 | 5 | 88 |
21 | 32.9 | 75.3 | 28.4 | 10 | 94 |
28 | 33.5 | 76.1 | 28.3 | 12 | 96 |
Mean values of double determination
time | reference substance | substance | ||||
reference DOC (mg/l) | blank DOC (mg/l) | reduction % | substance DOC (mg/l) | blank DOC (mg/l) | reduction % | |
-1 | 156.4 | 154.0 | ||||
0 h | 157.1 | 6.4 | 3.6 | 156.7 | 6,.4 | 2.4 |
3 h | 156.7 | 5.2 | 3.1 | 157.7 | 5.2 | 1.0 |
2 d | 154.5 | 6.9 | 5.6 | 152.4 | 6.9 | 5.5 |
5 d | 143.5 | 11.5 | 15.6 | 141.5 | 11.5 | 15.6 |
7 d | 137.5 | 12.8 | 20.3 | 138.4 | 12.8 | 18.4 |
9 d | 73.4 | 10.6 | 59.8 | 136.0 | 10.6 | 18.6 |
12 d | 7.9 | 5.5 | 98.5 | 136.8 | 5.5 | 14.7 |
13 d | 7.0 | 5.1 | 98.8 | - | - | - |
14 d | 8.3 | 4.0 | 97.3 | 145.4 | 4.0 | 8.2 |
15 d | 7.7 | 5.2 | 98.4 | - | - | - |
16 d | 6.1 | 4.2 | 98.8 | 142.8 | 4.2 | 10.0 |
19 d | - | - | - | 141.7 | 3.4 | 10.2 |
21 d | - | - | - | 139.0 | 4.5 | 12.7 |
23 d | - | - | - | 135.5 | 3.6 | 14.4 |
26 d | - | - | - | 132.0 | 3.4 | 16.5 |
28 d | - | - | - | 127.7 | 3.7 | 19.5 |
Description of key information
Not ready biodegradable.
Key value for chemical safety assessment
Additional information
Biodegradablility potential of the substance was based on a weight of evidence approach using available experimental data, in particular inherent biodegradability data and BOD5/COD values.
Inherent biodegradability studies were carried out following OECD guideline 302B, using test substances with high purity.
In the study dated 1994, biodegradability based on DOC removal was below the threshold value of 20 % after 28 days, thus the substance was considered as non inherently biodegradable.
In the study from 2007, biodegradability based on DOC removal was 30 %, after 28 days, thus the substance resulted as inherently biodegradable.
In both studies, blank control and positive control (diethylenglycole) were run and were considered as valid.
In addition, BOD5/COD values of ca. 0 were available on samples with purities ranging from 70 to 82 %, but no details on test procedures and results were available.
Based on available experimental data, the substance was considered as non ready biodegradable. Differently, no definite conclusion could be reached on the inherent biodegradability potential, due to biodegradability values overlapping the threshold value of 20 %.
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