Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 917-830-2 | CAS number: 1186514-91-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 13 June 2016 to 20 June 2016
- Reliability:
- 1 (reliable without restriction)
- Reason / purpose for cross-reference:
- other: Basis for representation of test solution concentrations
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- The test substance is listed as ZEF 6099/100. The test substance, a brown liquid, is the reaction product of C16-C18 and C18 unsaturated fatty acids, with (3,4-epoxycyclohexyl) methyl 3,4-epoxycyclohexylcarboxylate and neodecanoic fatty acid, oxiranylmethylester. The test substance was obtained from the sponsor and was stored at room temperature (15.2 – 23.3°C) in a closed vessel dark and dry.
- Analytical monitoring:
- yes
- Vehicle:
- no
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- The original animals of the test species of Danio raerio HAMILTON-BUCHANAN were originally obtained in March 2009 from Umweltbundesamt (UBA) and used for breeding and production of eggs. Sexually immature offspring (length 2± 1 cm) were used in the test.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- The hardness of the drinking water used in the test was 1.03 mmol/L.
- Test temperature:
- The temperature of the test medium was 22.2°C to 24.8 °C.
- pH:
- The pH during the test measured between 7.6 and 7.9 and complied with the requirements of the protocol (not varying by more than 1 unit).
- Dissolved oxygen:
- The dissolved oxygen during the test measured between 6.5 and 9.0 mg/L (at least 80%) and complied with the requirements of the protocol (at least 60% throughout the test).
- Salinity:
- No information provided.
- Conductivity:
- The conductivity of the drinking water used in the test measured 242 µS/cm at 25°C.
- Nominal and measured concentrations:
- The experimental test was performed with one nominal ZEF 6099/100 limit concentration of 100 mg/L; the test solution was prepared using the water-accommodated fraction. The decision to test one nominal concentration was based on the results of the algae study (Muckle, 2016). Analytical monitoring was conducted on the basis of dissolved organic carbon (DOC). Because of the low solubility of the test item in the test medium, the measured DOC concentrations at the beginning of the study in the treatment were in a similar low range as the blank control. Because of the low solubility in the test medium, no calculation from carbon content of the test item (72.42%) and DOC measurement was possible. The measured DOC concentrations in the treatment were in a similar range as the blank control. Therefore, the determination of the biological results was based on the nominal concentration. In an algal test with the same test item (Muckle 2016), the measured DOC concentrations were also in the same range as in the blank control, but toxicity at the nominal concentration of 100 mg/L showed the presence of dissolved test item in the test solutions. The concentration of minerals in the algal test medium and fish test medium is similar. Therefore, it can be concluded that the fishes were exposed to the test item at the nominal concentration of 100 mg/L.
- Details on test conditions:
- The content of the test item in the test solutions was calculated based on the organic carbon content of the test item and dissolved organic carbon (DOC) measurement. DOC was determined as total carbon (TC) minus IC (inorganic carbon) in the filtrated (0.45 µm) test solutions. Analyses were made using the carbon analyser TOC multi N/C 2100S, Analytik Jena.
- Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- The toxicity against Danio rerio was tested using a static design for 96 hr. No mortality was observed in the blank control and in the treatment at the one nominal limit concentration of 100 mg/L.
- Validity criteria fulfilled:
- yes
- Conclusions:
- No mortality was observed in the blank control and in the treatment at the one nominal limit concentration of 100 mg/L. The 96 h NOEC was ≥ 100 mg/L and the 96 hr LC50 was > 100 mg/L for mortality based on the nominal concentration.
Reference
Description of key information
The key study was a static acute fish toxicity test on the effects of ZEF 6099/100 on the freshwater fish Danio rerio.The 96-h NOEC was ≥100 mg/L (based on the water-accommodated fraction); the 96-h LOEC was >100 mg/L (based on the water-accommodated fraction); and the 96-h LC50 was >100 mg/L (based on the water-accommodated fraction).
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.