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EC number: - | CAS number: 56388-48-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Remarks:
- study on C12 naphthalene
- Adequacy of study:
- key study
- Study period:
- from 21 January 2013 to April 11, 2013
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: guidance test with GLP compliance
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Qualifier:
- according to guideline
- Guideline:
- other: Commission Regulation (EC) No 440/2008 of 30 May 2008, Part C: Methods for the determination of ecotoxicity, Publication No. L142, C.1. "Acute Toxicity for Fish".
- Qualifier:
- according to guideline
- Guideline:
- ISO 7346-1 (Determination of the Acute Lethal Toxicity of Substances to a Freshwater Fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)] - Part 1: Static Method)
- Qualifier:
- according to guideline
- Guideline:
- other: Guidance document on aquatic toxicity testing of difficult substances and mixtures, OECD series on testing and assessment number 23, December 14, 2000.
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
no data - Analytical monitoring:
- yes
- Details on sampling:
- Frequency: at t=0 h, t=24 h and t=96 h
Volume: 1 ml
Storage: Samples were stored in a freezer until analysis - Vehicle:
- no
- Details on test solutions:
- Preparation of test solutions
All test solutions were individually prepared. Preparation of test solutions started with loading rates that were magnetically stirred for one day to ensure reaching maximum dissolution in test medium. This resulted in clear solutions containing a floating layer. Consequently, these were left to settle fo
another day after which the clear and colourless Water Accommodated Fractions (WAFs) were collected by means of siphoning. The final test solutions were all clear and colourless. - Test organisms (species):
- Cyprinus carpio
- Details on test organisms:
- Species:Carp (Cyprinus carpio, Teleostei, Cyprinidae) Linnaeus, 1758
Source: Zodiac, proefacc, "De Haar Vissen", Wageningen University and Research Centre, The Netherlands
Mean length: Range-finding test: 2.3 ± 0.1 cm, Final test: 2.3 ± 0.2 cm
Mean weight: Range-finding test: 0.31 ± 0.07 g, Final test: 0.20 ± 0.07 g
Characteristics: F1 from a single parent-pair bred in UV-treated water
Total fish used: 23 - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 180 mg CaCO3 per litre
- Test temperature:
- 21.3-22.1 °C
- pH:
- 7.5-7.8
- Dissolved oxygen:
- 7.0-9.7 mg/l
- Salinity:
- no data
- Nominal and measured concentrations:
- nominal: A WAF prepared at a loading rate of 100 mg/l
measured: 0.020 mg/l - Details on test conditions:
- Controls: Test medium without test substance or other additives
Test vessels: 6.5 litres, all-glass, containing 4 litres of test solution
Test medium: Adjusted ISO medium with a hardness of 180 mg CaCO3 per litre and a pH of 7.7 ± 0.3
Number of fish: 7 per concentration
Loading: 0.35 g fish/litre, i.e. 7 fish per 4 litres of test medium
Illumination: 16 hours photoperiod daily
Aeration: The test media were not aerated during the test
Feeding: No feeding from 24 hours prior to the test and during the total test period
Introduction of fish: Within 10 minutes after preparation of the test media from a holding tank with comparable water quality parameters and pH and temperature differences between test and holding tank media of less than 0.5 unit and 0.5 °C
Euthanasia: At the end of the test the surviving fish were rapidly killed by exposing them to ca. 1.2 % ethylene glycol monophenylether in water - Reference substance (positive control):
- yes
- Remarks:
- pentachlorophenol (PCP)
- Duration:
- 96 h
- Dose descriptor:
- NOELR
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 0.02 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- Analysis of the samples taken from the WAF prepared at 100 mg/l showed measured concentrations ranging between 22.5 and 33.5 µg/l for peak 1. For peak 2 concentrations measured ranged between 16.1 and 24.6 µg/l. The average exposure concentration was based on the measured concentrations of peak 2, being a worst-case approach (lowest concentrations measured). The average measured concentration corresponded with 20 µg/l.
No effects were observed at the limit concentration tested. - Results with reference substance (positive control):
- The 96 h-LC50 for carp exposed to PCP was 0.17 mg/l (95 % confidence interval between 0.15 and 0.27 mg/l). The range of the 96 h-LC50 for carp is generally between 0.10 and 0.46 mg/l based on historical data of reference tests performed approximately every 3 months from April 1988 until the end of 2000, and annually since then. Hence, the sensitivity of carp originating from the present batch for PCP falls within the range of sensitivities generally observed during the past years.
- Reported statistics and error estimates:
- No statistical anaysis was used because the test substance proved to be non-toxic (LC50 > maximum soluble concentration).
- Sublethal observations / clinical signs:
Range-finding test
Based on these results samples taken from the WAF prepared at 100 mg/l were analysed. The concentrations measured based on peak 1 were 4.8, 19.4 and 24.2 µg/l at the start, after 24 hours and after 96 hours, respectively. Based on peak 2 concentrations measured were <4.0, 13.3 and 8.2 µg/l at the start, after 24 hours and after 96 hours, respectively. It was unknown why the concentration measured at the start of the test was lower than what was measured at later time points. A sample taken from the WAF prepared at 10 mg/l showed a slightly higher concentration of 11.9 (peak 1) and 8.5 µg/l (peak 2) at the study start. The LC50 exceeded the limit of solubility in test medium with an expected actual concentration of approximately 10-20 µg/l.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 96 h-LC50 exceeded 0.020 mg/l, being the average measured exposure concentration in a WAF prepared at a loading rate of 100 mg/l.
- Executive summary:
The fish acute toxicity test was performed according to OECD guideline No. 203 under GLP compliance. The result showed the test substance induced no visible or lethal effects in carp at 0.020 mg/l, being the average measured exposure concentration in a WAF prepared at a loading rate of 100 mg/l (NOELR). The 96 h-LC50 exceeded 0.020 mg/l, being the average measured exposure concentration in a WAF prepared at a loading rate of 100 mg/l (LL50).
- Endpoint:
- short-term toxicity to fish
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across: supporting information
- Remarks:
- read-across details
- Dose descriptor:
- LL50
- Effect conc.:
- > 100 mg/L
- Remarks on result:
- not measured/tested
- Remarks:
- based on read-across
- Conclusions:
- Based on read-across it is concluded that the LL50 of the substance is >100 mg/L
Referenceopen allclose all
Description of key information
In a study on the analogue the 96 h-LC50 exceeded 0.020 mg/l, being the average measured exposure concentration in a WAF prepared at a loading rate of 100 mg/l.
The LL50 is 100 mg/L.
The data on the analogue can be used as read-across to assess the toxicity towards fish (see section 13)
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
The fish acute toxicity test was performed according to OECD guideline No. 203 under GLP compliance. The result showed the test substance induced no visible or lethal effects in carp at 0.020 mg/l, being the average measured exposure concentration in a WAF prepared at a loading rate of 100 mg/l (NOEC). The 96 h-LC50 exceeded 0.020 mg/l, being the average measured exposure concentration in a WAF prepared at a loading rate of 100 mg/l.
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