Reaction mass of disodium [2,2'-(ethane-1,2-diylbis{[2-(hydroxy-kO)benzyl]imino-kN})diacetato-kO(4-)]zinc(2-) and sodium chloride

Short-term toxicity to fish

Description of key information

The LC50 values after 24, 48, 72, and 96 hours of the exposure determined on the basis of the nominal concentration of the test item are >100.0 mg/L. The LC0/96h is  100.0 mg/L, whereas the LC100/96h is > 100.0 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L

Additional information

The acute toxicity study of the test item, ZnHBED on the rainbow trout was conducted as a static test. The fish (Oncorhynchus mykiss Walb.) were exposed to the test item at the limit test concentration of 100.0 mg/L and a control (0.0 mg/L). The exposure took place in glass aquaria with a capacity of 10 L. There was one replicate of the test concentration and the control. Ten fish were introduced into each aquarium. The fish were observed for mortality and intoxication symptoms after 3, 6, 24, 48, 72, and 96 hours of the exposure. All fish in the control and in the test concentration survived the 96-hour exposure. They did not exhibit any intoxication symptoms. Chemical analyses were performed using a validated method. Samples collected at the exposure initiation (t0) and at the exposure termination (t96) were analyzed. The average concentration of ZnHBED in samples collected at the exposure initiation (t0) was 95.3% of the nominal concentration. The average concentration of ZnHBED in samples collected at the exposure termination (t96) was 88.8% of the initial concentration. Since the concentration of ZnHBED in the solution was higher than 80% of the initial concentration after 96 hours, the LCx values were calculated on the basis of the nominal concentration of the test item.