Mecoprop

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Wistar rats were administered the test material via a single injection into the abdominal cavity. Two doses were tested.

GLP compliance:

yes

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Mecoprop

EC Number:

230-386-8

EC Name:

Mecoprop

Cas Number:

7085-19-0

Molecular formula:

C10H11ClO3

IUPAC Name:

2-(4-chloro-2-methylphenoxy)propanoic acid

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Age at study initiation: About 12 Weeks
- Weight at study initiation: Mean Female 177 g (700 mg/kg), 169 g (200 mg/kg). Mean Male 170 g (700 mg/kg), 185 g (200 mg/kg)
- Fasting period before study: 16 hours
- Housing: Animals were housed in stainless steel wire mesh cages. 5 animals per cage.
- Water: Tap water ad libitum
- Acclimation period: At least one week

ENVIRONMENTAL CONDITIONS
- Temperature: 20-24 °C
- Humidity: 30-70
- Photoperiod: 12 h/12 h (6.00 - 18.00 hours/ 18.00 - 6.00 hours)

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

CMC (carboxymethyl cellulose)

Details on exposure:

TEST MATERIAL:
- Vehicle: 0.5 % aqueous carboxymethyl cellulose
- Amount applied: 10 mL/kg for each dose
- Concentration: 7 (700) and 2 % w/v (200 mg/kg).

MODE OF ADMINISTRATION:
- Single injection into the abdominal cavity

Doses:

200 and 700 mg/kg body weight

No. of animals per sex per dose:

5 female and 5 male Wistar rats per dose

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: 15 Days
- Frequency of observations and weighing: Recording of signs several times on the day of administration and at least once each workday. Check for moribund and dead animals twice each workday and once on weekends and public holidays. Weight recorded at beginning of test, Day 2, Day 7 and Day 13.
- Necropsy of survivors performed: yes, withdrawal of feed 16 hours before sacrifice with CO2; then necropsy with gross-pathological assessment. All animals that died were necropsied as early as possible.

Results and discussion

Effect levels

Key result

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 200 - < 700 mg/kg bw

Based on:

test mat.

Mortality:

No mortality was observed at dose 200 mg/kg bw for both male and female groups. At dose level 700 mg/kg bw all animals in both male and female groups were deceased after day 1.

Clinical signs:

At both dosages, there were observed clinical abnormalities which included dyspnea, apathy, staggering, spastic gait and poor general state. At the higher dosage level, more clinical abnormalities were observed including, abnormal positioning, atonia, missing pain reflex, narcotic-like state, ruffled fur and exsiccosis.

Body weight:

The mean body weights at 200 mg/kg bw increased during the observation period.

Gross pathology:

Animals that died: Abdomen: intra-abdominal collection of moderately clear liquids.
Sacrificed animals: Liver: intra-abdominal conglutinations; blunt margins. Spleen: serosa opalescent.

Any other information on results incl. tables

Mortality During the Study

Dose (mg/kg)		700	200
Males
Number of Animals		5	5
Dead Animals After	1 h	0	0
	1 d	5	0
	2 d	5	0
	7 d	5	0
	14 d	5	0
Females
Number of Animals		5	5
Dead Animals After	1 h	0	0
	1 d	5	0
	2 d	5	0
	7 d	5	0
	14 d	5	0

Applicant's summary and conclusion

Conclusions:

Under the conditions of this study, the acute LD50 after a single intraperitoneal administration of test material in male and female rats was found to be > 200 < 700 mg/kg.

Executive summary:

The acute toxicity of the test material after a single intraperitoneal administration was investigated, under GLP conditions.

5 male and 5 female Wistar rats per dose group were administered 200 and 700 mg/kg body weight by a single injection into the abdominal cavity. The test material was prepared with 0.5% aqueous carboxymethyl cellulose. Unspecific clinical symptoms were observed. Mortality occurred in the dosage of 700 mg/kg. Necropsy findings in animals that died: moderately clear liquids in the abdomen. In sacrificed animals: liver: intraabdominal conglutinations, blunt margins; spleen: serosa opalescent. In dosage 200 mg/kg no mortality occurred (LD0 = 200 mg/kg) and symptoms occurred at each dosage (ED0 = < 200 mg/kg).

Under the conditions of this study, the acute LD50 after a single intraperitoneal administration of test material in male and female rats was found to be > 200 < 700 mg/kg.