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EC number: 204-844-2 | CAS number: 127-47-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Acceptable, well-documented study.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 979
- Report date:
- 1979
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Principles of method if other than guideline:
- BASF Test
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- Retinyl acetate
- EC Number:
- 204-844-2
- EC Name:
- Retinyl acetate
- Cas Number:
- 127-47-9
- Molecular formula:
- C22H32O2
- IUPAC Name:
- (2E,4E,6E,8E)-3,7-dimethyl-9-(2,6,6-trimethylcyclohex-1-en-1-yl)nona-2,4,6,8-tetraen-1-yl acetate
- Details on test material:
- Vitamin A Acetat-Mutterlaugenöl, 1.50 mio IU/g (ca. 520 mg Vitamin A acetate/g or 52%); 40% all-trans retinol acetate (CAS No. 127-47-9), remainder isomeric retinol acetate(s); no further data
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Wiga, Sulzfeld
- Weight at study initiation: Mean body weight was 180-200 g for males and 160-180 g for females.
- Fasting period before study: 15H - 20H before application
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- other: olive oil
- Details on oral exposure:
- The test substance was administered as different volumes of a 70% solution in olive oil.
MAXIMUM DOSE VOLUME APPLIED: 21 ml/kg - Doses:
- Group 1: 14700 mg/kg bw (21.0 ml/kg)
Group 2: 12100 mg/kg bw (17.3 ml/kg)
Group 3: 10000 mg/kg bw (14.3 ml/kg)
Group 4: 8250 mg/kg bw (11.8 ml/kg)
Group 5: 6810 mg/kg bw (9.7 ml/kg) - No. of animals per sex per dose:
- Group 1: 4 males, 5 females
Group 2: 5 males, 4 females
Group 3: 4 males, 4 females
Group 4: 5 males, 5 females
Group 5: 5 males, 5 females - Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Mortality after 1 hour and after 1, 2, 7, and 14 days was recorded. Clinical observations and body weight were recorded. At the end of the observation period, the surviving animals were sacrificed. Decedents and survivors were subjected to autopsy.
- Necropsy of survivors performed: yes - Statistics:
- Probit Analysis
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 9 560 mg/kg bw
- Mortality:
- see table
- Clinical signs:
- other: Signs of toxicity were observed at all dose levels. 14700 mg/kg bw: dyspnea, stertorous breathing, apathy, staggering, tremors, spastic gait, piloerection, diarrhea, exsiccosis, encrustations at the snout and eyes, poor general state 12100 mg/kg bw:
- Gross pathology:
- Pathological examination of the animals that died revealed changes of the heart (acute dilatation, congestive hyperemia), liver (discoloration, enlargement of the peripheral lobes), and gut (atony, diarrhotic contents). No pathological changes were observed in surviving animals.
Any other information on results incl. tables
Table: mortality rates
dose * sex * mortality within
[mg/kg]
* 1h * 1d * 2d * 7d * 14d
14700 * males * 0/4 * 0/4 * 2/4 * 4/4 * 4/4
14700 * females * 0/5 * 0/5 * 2/5 * 5/5 * 5/5
12100 * males * 0/5 * 0/5 * 1/5 * 5/5 * 5/5
12100 * females * 0/4 * 0/4 * 0/4 * 4/4 * 4/4
10000 * males * 0/4 * 0/4 * 0/4 * 2/4 * 2/4
10000 * females * 0/4 * 0/4 * 0/4 * 1/4 * 1/4
8250 * males * 0/5 * 0/5 * 0/5 * 0/5 * 0/5
8250 * females * 0/5 * 0/5 * 0/5 * 2/5 * 2/5
6810 * males * 0/5 * 0/5 * 0/5 * 1/5 * 1/5
6810 * females * 0/5 * 0/5 * 0/5 * 0/5 * 0/5
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.