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Diss Factsheets
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EC number: 944-003-3 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study carried out essentially according to a guideline, without detailed documentation. Incubation in sealed vessels due to volatility of CS2
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- yes
- Remarks:
- The test was carried out in sealed infusion bottles to prevent CS2 from escaping. The presence or absence of a headspace was not reported but is presumed to allow for sufficient CO2 for the biomass to grow normally.
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- not available
- Test organisms (species):
- Chlorella pyrenoidosa
- Details on test organisms:
- no data
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 90 mg CaCO3/L
- Test temperature:
- 20 +/- 1 deg C
- pH:
- 8.7 +/- 0.2
- Dissolved oxygen:
- Not reported
- Nominal and measured concentrations:
- Nominal: 0 mg/l; 3.2 mg/l; 5.6 mg/l; 10 mg/l; 18.0 mg/l; 32 mg/l each presumed from Fig. 4 in the publication.
- Details on test conditions:
- The test was carried out in sealed infusion bottles to prevent evaporation of CS2. The presence or absence of a headspace was not reported but is presumed to allow for sufficient CO2 for the biomass to grow normally. The medium contained major salts, urea, Ca-glycerophosphate, dextrose and trace elements. The complete composition of the test medium is given in Van Leeuwen and Maas, Environ. Pollution A37: 105-115 (1985). The starting density of the cells was 1E5 cells/mL. The volume of test medium was 100 mL per flask. The mediu was shaken under fluorescent light (7.5 W/m2).
- Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- 21 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- 554 mg/L
- Nominal / measured:
- estimated
- Conc. based on:
- other: EC# 944-003-3
- Basis for effect:
- growth rate
- Remarks on result:
- other: See attached background material
- Reported statistics and error estimates:
- no data
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The 96-h EC50 of CS2 for the alga Chlorella pyrenoidosa in sealed infusion bottles was 21 mg/L, based on the inhibition of growth rate. From these data, the toxicity of the substance being registered (EC# 944-003-3) is estimated to be 554 mg/l.
- Executive summary:
A test in sealed infusion bottles was carried out to determine the toxicity of CS2 to the alga Chlorella pyrenoidosa. The test was similar to the OECD Guideline 201, but was not carried out in compliance with GLP. The 96-h ErC50 for Chlorella pyrenoidosa was 21 mg/L. From these data, the toxicity of the substance being registered (EC# 944-003-3) is estimated to be 554 mg/l.
Reference
Description of key information
The substance degrades rapidly at pH <11 to form highly volatile Carbon disulphide (CS2). A test in sealed infusion bottles was carried out to determine the toxicity of CS2 to the alga Chlorella pyrenoidosa. The test was similar to the OECD Guideline 201, but was not carried out in compliance with GLP. The 96-h ErC50 for Chlorella pyrenoidosa was 21 mg/L. From these data, the toxicity of the substance being registered (EC# 944-003-3) is estimated to be 554 mg/l. The basis for this estimate is attached as background material in the supporting study (read-across to CS2).
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 554 mg/L
Additional information
The substance degrades rapidly at pH <11 to form highly volatile Carbon disulphide (CS2). A test in sealed infusion bottles was carried out to determine the toxicity of CS2 to the alga Chlorella pyrenoidosa. The test was similar to the OECD Guideline 201, but was not carried out in compliance with GLP. The 96-h ErC50 for Chlorella pyrenoidosa was 21 mg/L. From these data, the toxicity of the substance being registered (EC# 944-003-3) is estimated to be 554 mg/l. The basis for this estimate is attached as background material in the supporting study (read-across to CS2).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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