Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 274-159-1 | CAS number: 69852-45-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 982
- Report date:
- 1982
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 3-[[3-(dimethylamino)propyl]amino]propiononitrile
- EC Number:
- 274-159-1
- EC Name:
- 3-[[3-(dimethylamino)propyl]amino]propiononitrile
- Cas Number:
- 69852-45-5
- Molecular formula:
- C8H17N3
- IUPAC Name:
- 3-{[3-(dimethylamino)propyl]amino}propanenitrile
- Reference substance name:
- 3,3'-[[3-(dimethylamino)propyl]imino]bispropiononitrile
- EC Number:
- 275-331-9
- EC Name:
- 3,3'-[[3-(dimethylamino)propyl]imino]bispropiononitrile
- Cas Number:
- 71326-27-7
- Molecular formula:
- C11H20N4
- IUPAC Name:
- 3,3'-{[3-(dimethylamino)propyl]imino}dipropanenitrile
- Test material form:
- liquid
- Remarks:
- colorless
Constituent 1
impurity 1
- Specific details on test material used for the study:
- Batch no. Zd 429-10A III
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2
- Metabolic activation:
- with and without
- Metabolic activation system:
- Rat liver microsomes and co-factors
- Test concentrations with justification for top dose:
- 5, 10, 50, 100, 500 ,1000 and 5000 µg/0.1 ml
- Vehicle / solvent:
- Twice distilled water
Controls
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- 2-nitrofluorene
- N-ethyl-N-nitro-N-nitrosoguanidine
- other: DMSO
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: Minimum agar, plus salts and glucose.
The plates were incubated for about 48 hours at 37°C in darkness. - Evaluation criteria:
- When the colonies had been counted, the arithmetic mean was calculated. The test substance is generally considered to be non-mutagenic if the colony count in relation to the negative control is not doubled at any concentration.
Results and discussion
Test results
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
Any other information on results incl. tables
Material |
Concentration of testing sample (µg / plate) |
Presence of S9 Mix |
Back Mutation Number of colunies/plate (mean) |
|||||
Base Conversion Type |
Frame Shift Type |
|||||||
TA100 |
TS1535 |
WP2UBrA |
TA98 |
TA1537 |
TA1538 |
|||
Vehicle Control |
|
- |
91 |
15 |
20 |
19 |
15 |
14 |
TK12271
|
5 |
- |
87 |
18 |
20 |
28 |
14 |
18 |
10 |
- |
89 |
20 |
26 |
29 |
14 |
14 |
|
50 |
- |
95 |
23 |
15 |
27 |
9 |
12 |
|
100 |
- |
100 |
15 |
19 |
25 |
14 |
20 |
|
500 |
- |
86 |
15 |
21 |
20 |
13 |
14 |
|
1000 |
- |
83 |
15 |
20 |
25 |
15 |
14 |
|
5000 |
- |
88 |
15 |
19 |
25 |
12 |
14 |
|
Vehicle Control |
|
+ |
78 |
11 |
16 |
46 |
8 |
33 |
TK12271
|
5 |
+ |
71 |
12 |
24 |
44 |
7 |
30 |
10 |
+ |
76 |
10 |
21 |
42 |
5 |
29 |
|
50 |
+ |
83 |
12 |
13 |
44 |
5 |
27 |
|
100 |
+ |
78 |
12 |
17 |
46 |
12 |
26 |
|
500 |
+ |
81 |
15 |
18 |
51 |
6 |
30 |
|
1000 |
+ |
81 |
12 |
13 |
44 |
6 |
28 |
|
5000 |
+ |
69 |
7 |
19 |
45 |
9 |
30 |
Applicant's summary and conclusion
- Conclusions:
- In the experiments performed without and with microsomal activation, comparison of the number of histidine- or tryptophan-prototrophic mutants in the controls and after treatment with TK 12271 revealed no marked differences.
- Executive summary:
TK 12271 was tested for mutagenic effects on histidine-auxotrophic strains of Salmonella typhimirium and on a tryptophan-auxotrophic strain of E. coli. The investigations were performed with the following concentrations of the trial substance without and with microsomal activation: 5, 10, 50, 100, 500, 1000 and 5000 µg/0.1 ml.
These tests permit the detection of point mutations in bacteria induced by chemical substances. Any mutagenic effects of the substances are demonstrable on comparison of the number of bacteria in the treated and control cultures that have undergone back- mutation to histidine- or tryptophan- prototrophism. To ensure that mutagenic effects of metabolites of the test substance formed in mammals would also be detected, experiments were performed in which the cultures were additionally treated with an activation mixture (rat liver microsomes and co-factors).
In the experiments performed without and with microsomal activation, comparison of the number of back-mutant colonies in the controls and the cultures treated with the various concentrations of TK 12271 revealed no marked deviations.
No evidence of the induction of point mutations by TK 12271 or by the metabolites of the substance formed as a result of microsomal activation was detectable in the strains ofS. typhimuriumandE. coliused in these experiments.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.