N,N'-hexamethylenebis(cinnamylideneamine)

Endpoint summary

Description of key information

Short-term toxicity to aquatic invertebrates

EC50 (48h) = 2.28 mg/l

Toxicity to aquatic algae and cyanobacteria

E_rC50 = 5.31 mg/l

Additional information

Short-term toxicity to aquatic invertebrates

The median effect concentration (EC50) and the no-effect concentration (NOEC) of test item to Daphnia magna were investigated under semi-static exposure conditions over a period of 48 h, following OECD guideline 202.

Since the test item is readily biodegradable, the test was performed under semi-static exposure conditions with renewal of the test medium after 24 h of exposure.

The nominal loading rates corresponding to the different dilutions were 100, 45.5, 20.7, 9.39 and 4.27 mg/l.

40 individual Daphnia divided into 2 test vessels were exposed to each concentration of the test item, and 60 Daphnia divided into 3 test vessels were used as blank controls.

The concentrations of Intercure 3 in the test medium were measured by HPLC analysis at the beginning and after 24 and 48 h of exposure. These analyses confirmed that the test item was partly dissolved, that the concentrations were not stable over the whole 48 h period and that they did not remain within 80-120 % of the nominal loading rates. Therefore, the effective concentrations EC_xwere assessed based on measured concentrations. The 48 h EC50 value of Intercure 3 of Daphnia magna was 2.28 mg/l. This value is based on measured concentrations. All validity criteria were fulfilled.     

Toxicity to aquatic algae and cyanobacteria

The effective concentrations (E_rC₁₀, E_rC₅₀and E_yC₁₀, E_yC₅₀) as well as the no observed effect concentrations (NOE_rC and NOE_yC), of test item to the green alga Desmodesmus subspicatus based on the average specific growth rate and the yield (algal biomass production) were investigated over a period of 72 h, according to the OECD guideline 201.

The test solutions were prepared by respective dilutions of a saturated stock solution.

The test concentrations were 100, 31.6, 10.0, 3.16 and 1.00 mg/l loading rate. These loadings refer to the corresponding dilutions (spacing factor 3.16) of a 100 mg/l saturated stock solution.

Three parallel test vessels were used for each test concentration of the test item and six vessels for the blank controls.

The 72 h E_rC₅₀and E_yC₅₀values of Intercure 3 on the green alga Desmodesmus subspicatus were 5.31 mg/l and 0.698 mg/l, respectively. These values are based on the geometric mean of the measured concentrations.

Justification for classification or non-classification

According to the CLP Regulation (EC n. 1272/2008), Part 4: Environmental Hazards, the substances can be classified for hazardous to the aquatic environment (fish toxicity) when the following criteria are met:

A )Acute (short-term) aquatic hazard

Category Acute 1:

96 hr LC 50 (for fish) ≤ 1 mg/l and/or

48 hr EC 50 (for crustacea) ≤ 1 mg/l and/or

72 or 96 hr ErC 50 (for algae or other aquatic plants) ≤ 1 mg/l

The EC50 value calculated in a 48 h semi-static test on Daphnia magna was 2.28 mg/l, This result is confirmed by the one obtained in the toxicity test to algae, where the ErC50 value calculated was established to be 5.31 mg/l. Based on the results obtained in the short term toxicity for invertebrates and algae and in the biodegradability tests, the substance is Not classified for aquatic toxiciy according to the CLP Regulation