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EC number: 208-358-1 | CAS number: 524-38-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- (Q)SAR
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- results derived from a valid (Q)SAR model and falling into its applicability domain, with limited documentation / justification
- Justification for type of information:
- The supporting QMRF report has been attached
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Principles of method if other than guideline:
- Prediction is done using OECD QSAR Toolbox version 3.4 with logKow as the primary descriptor.
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- - IUPAC name: 2-hydroxy-1H-isoindole-1,3(2H)-dione
- Molecular formula: C8H5NO3
- Molecular weight: 163.132 g/mol
- Smiles notation: c12c(C(=O)N(C1=O)O)cccc2
- InChl: 1S/C8H5NO3/c10-7-5-3-1-2-4-6(5)8(11)9(7)12/h1-4,12H
- Substance type: Organic
- Physical state: Solid - Analytical monitoring:
- not specified
- Vehicle:
- not specified
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 72 h
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 109.156 mg/L
- Nominal / measured:
- estimated
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The median Effective concentration (EC50) value for 2-hydroxy-1H-isoindole-1,3(2H)-dione on Pseudokirchneriella subcapitata in a 72 hour study was estimated to be 109.15 mg/L on the basis of effects on growth rate.
- Executive summary:
Based on the prediction done using the OECD QSAR toolbox version 3.4 with log kow as the primary descriptor and considering the five closest read across substances, the short term toxicity on aquatic algae and cyanobacteria was predicted for target substance 2-hydroxy-1H-isoindole-1,3(2H)-dione
(CAS no. 524 -38 -9). EC50 value was estimated to be 109.15 mg/l for Pseudokirchneriella subcapitata for 72 h duration. Based on this value it can be concluded that the substance 2-hydroxy-1H-isoindole-1,3(2H)-dione is considered to be non-toxic to aquatic environment and can be considered to be not classified as per the CLP classification criteria.
Reference
The
prediction was based on dataset comprised from the following
descriptors: EC50
Estimation method: Takes average value from the 5 nearest neighbours
Domain logical expression:Result: In Domain
(((("a"
or "b" or "c" or "d" or "e" )
and ("f"
and (
not "g")
)
)
and ("h"
and (
not "i")
)
)
and ("j"
and "k" )
)
Domain
logical expression index: "a"
Referential
boundary: The
target chemical should be classified as Imides (Acute toxicity) by
US-EPA New Chemical Categories
Domain
logical expression index: "b"
Referential
boundary: The
target chemical should be classified as Aryl AND Fused saturated
heterocycles AND Hydroxamic acid AND Imide AND N-Hydroxylamine
derivatives by Organic Functional groups
Domain
logical expression index: "c"
Referential
boundary: The
target chemical should be classified as Aryl AND Fused saturated
heterocycles AND Hydroxamic acid AND Imide AND Overlapping groups by
Organic Functional groups (nested)
Domain
logical expression index: "d"
Referential
boundary: The
target chemical should be classified as Amide, aromatic attach [-C(=O)N]
AND Aromatic Carbon [C] AND Carbonyl, olefinic attach [-C(=O)-] AND
Carbonyl, one aromatic attach [-C(=O)-] AND Hydroxy, nitrogen attach
[-OH] AND Miscellaneous sulfide (=S) or oxide (=O) AND Nitrogen, two or
tree olefinic attach [>N-] AND Olefinic carbon [=CH- or =C<] AND Oxygen,
nitrogen attach [-O-] AND Oxygen-subtution at N on [-CO-N-CO-] by
Organic functional groups (US EPA)
Domain
logical expression index: "e"
Referential
boundary: The
target chemical should be classified as Aromatic compound AND Carbonic
acid derivative AND Carboxylic acid derivative AND Heterocyclic compound
by Organic functional groups, Norbert Haider (checkmol)
Domain
logical expression index: "f"
Referential
boundary: The
target chemical should be classified as Non binder, without OH or NH2
group by Estrogen Receptor Binding
Domain
logical expression index: "g"
Referential
boundary: The
target chemical should be classified as Non binder, impaired OH or NH2
group OR Non binder, MW>500 by Estrogen Receptor Binding
Domain
logical expression index: "h"
Referential
boundary: The
target chemical should be classified as Non-Metals by Groups of elements
Domain
logical expression index: "i"
Referential
boundary: The
target chemical should be classified as Halogens by Groups of elements
Domain
logical expression index: "j"
Parametric
boundary:The
target chemical should have a value of log Kow which is >= 0.0495
Domain
logical expression index: "k"
Parametric
boundary:The
target chemical should have a value of log Kow which is <= 0.54
Description of key information
Based on the prediction done using the OECD QSAR toolbox version 3.4 with log kow as the primary descriptor and considering the five closest read across substances, the short term toxicity on aquatic algae and cyanobacteria was predicted for target substance 2-hydroxy-1H-isoindole-1,3(2H)-dione
(CAS no. 524 -38 -9). EC50 value was estimated to be 109.15 mg/l for Pseudokirchneriella subcapitata for 72 h duration. Based on this value it can be concluded that the substance 2-hydroxy-1H-isoindole-1,3(2H)-dione is considered to be non-toxic to aquatic environment and can be considered to be not classified as per the CLP classification criteria.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 109.15 mg/L
Additional information
Various predicted data for the target chemical 2-hydroxy-1H-isoindole-1,3(2H)-dione (CAS No. 524-38-9) and weight of evidence study for its read across substance were reviewed to summarize the following information:
Short term toxicity on aquatic algae and cyanobacteria of target chemical 2-hydroxy-1H-isoindole-1,3(2H)-dione(CAS No. 524-38-9) is predicted using the OECD QSAR toolbox version 3.4 with log kow as the primary descriptor and considering the five closest read across substances (2017).EC50 value was estimated to be 109.15 mg/l for Pseudokirchneriella subcapitata for 72 h duration. Based on this value it can be concluded that the substance 2-hydroxy-1H-isoindole-1,3(2H)-dione is considered to be non-toxic to aquatic environment and can be considered to be not classified as per the CLP classification criteria.
In another prediction done using the EPI Suite ECOSAR version 1.10 (EPI Suite, 2017), the short term toxicity on green algae was predicted for test substance2-hydroxy-1H-isoindole-1,3(2H)-dione(CAS No. 524-38-9).On the basis of effects observed in a static freshwater system, the effect concentration EC50 value for the substance is estimated to be 1101.824 mg/l for green algae for 96 hr duration. Based on this value, it can be concluded that the test chemical 2 -hydroxy-1H-isoindole-1,3(2H)-dione can be considered as non-toxic to green algae at environmentally relevant concentrations and can be considered not-classified as per the CLP classification criteria.
In a weight of evidence study from authoritative database (J-CHECK, 2016) of the read across chemicalPhthalimide(CAS no. 85-41-6),short term toxicity to algae study was carried out for 72 hrs for evaluating the effects of the test compound Phthalimide on green algae in a static fresh water system. On the basis of effect on growth rate of the test organism green algae, the 72 hr EC50 and NOEC value was determined to be > 150 and 1.1 mg/l and on the basis of areas under the growth curves, the 72 hr EC50 and NOEC value was determined to be 62 and 6.5 mg/l, respectively. Thus, based on the EC50 value (> 150 mg/l), it can be concluded that the substance Phthalimide can be considered as non-toxic to aquatic organisms and thus cannot be classified as hazardous as per the CLP criteria.
For the same read across chemical Phthalimide(CAS no. 85-41-6),short term toxicity to Selenastrum capricornutum study was carried out for 72 hrs (OECD SIDS, 2005). The study was performed according to OECD Guideline 201 (Alga, Growth Inhibition Test). The study was based on the effects of the read across compound Phthalimide on Selenastrum capricornutum in a static fresh water system at an average temperature of 22.1°C. Test chemical conc. used for the study were 0, 10.6, 19.1, 34.3, 61.7, 111.1 and 200.0 mg/l, respectively. 300 mL net triangle conical glass flask was used as a test vessel. The flasks were incubated in a shaking water bath at 22.1 degrees C with continuous illumination at 4000 - 5000 lux. Based on the effect on growth rate of the test organism Selenastrum capricornutum, the 72 hrs ErC50 and NOEC value was determined to be 161 and 10.7 mg/l, respectively. Thus, based on EC50 value, it can be concluded that the substance Phthalimide can be considered as non-toxic to aquatic organisms and thus cannot be classifiedas hazardous as per the CLP criteria.
In an another weight of evidence study for read across chemical N-(Cyclohexylthio)phthalimide (CAS no. 17796-82-6) (HSDB and High Production Volume (HPV) Challenge Program; 2016), short term toxicity to Selenastrum capricornutum (green algae) study was carried out for 96 hrs. The study was based on the effects of the read across compound N-(Cyclohexylthio)phthalimide on Selenastrum capricornutum (green algae) in a static fresh water system. The test algae were obtained from the US EPA Environmental Research Laboratory in Corvallis, Oregon. Test chemical conc. used for the study were 0, 6, 10, 32, 56 and 100 mg/l, respectively. Initial cell density of algae was 2 × 104cells/ml. All tests were carried out in triplicates. Statistical analysis involved converting each test concentration to a logarithm, and the corresponding percentage decrease of in vivo chlorophyll a or cell numbers was converted to a probit. The EC50 and 95% confidence limits were then calculated by linear regression. The toxicity of the test substance to algae was similar throughout the 96 hr exposure. There was no significant difference between growth of the control and solvent control cultures after 96 hr of exposure by either measured parameter. Based on effect on biomass and growth of the test organism Selenastrum capricornutum (green algae), the 96 hr EC50 value was determined to be 21 mg/l and on the basis of chlorophyll measurement, the 96 hr EC50 value was determined to be 22 mg/l and NOEC value was 6 mg/l, respectively. Thus, based on the EC50 value, it can be concluded that the substance N-(Cyclohexylthio)phthalimide can be considered as toxic to aquatic organisms and thus can be classified as aquatic chronic category 3. Since the chemical is readily biodegradable in nature, chemical N-(Cyclohexylthio)phthalimide can be considered as non-toxic to aquatic organisms and thus cannot be classified as hazardous as per the CLP criteria.
Thus, based on the overall reported results for target chemical2-hydroxy-1H-isoindole-1,3(2H)-dione(OECD QSAR toolbox version 3.4 and EPI suite, 2017) and for its read across substance (from authoritative database J-CHECK, HSDB andsecondary sourceHigh Production Volume (HPV) Challenge Program; 2016), it can be concluded that the test substance2-hydroxy-1H-isoindole-1,3(2H)-dionecan be considered asnon-toxic to aquatic environment and can be considered to be not classified as per the CLP classification criteria.
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