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EC number: 217-968-7 | CAS number: 2022-85-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2003-09-30 - 2003-11-28
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 003
- Report date:
- 2003
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Version / remarks:
- 1992
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Flucytosine
- EC Number:
- 217-968-7
- EC Name:
- Flucytosine
- Cas Number:
- 2022-85-7
- Molecular formula:
- C4H4FN3O
- IUPAC Name:
- flucytosine
- Test material form:
- solid: particulate/powder
Constituent 1
- Specific details on test material used for the study:
- Please refer to Test material information
Study design
- Oxygen conditions:
- aerobic
- Remarks:
- no aeration
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Supernatant from settled domestic activated sludge, Source: Galt Wastewater Treatment Plant, Cambridge, Ontario, Canada
Date Collected: 2003-10-31
Date Used: 2003-10-31
- Inoculum concentration: Approximately 1.0 mL/L - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 7.17 mg/L
- Based on:
- ThOD
- Remarks:
- Based on molecular weight, the calculated ThOD was 0.56 mg O2/mg substance.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: mineral medium, pH 7.2
- Additional substrate: reference substrate for inhibition testing: phthalic acid
- Solubilising agent (type and concentration if used): Dilution water: Deionized water with nutrients
- Test temperature: 22 +/- 2 °C
- pH: 7.4 +/- 0.2
- pH adjusted: no
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: Non-renewal, closed bottle; Glass, with ground glass stoppers and plastic seals; Test volume: 300 ml
- Number of culture flasks/concentration: 2 replicates
- Measuring equipment: Oxygen electrode
SAMPLING
- Sampling frequency: 7 day interval
CONTROL AND BLANK SYSTEM
- Toxicity control: reference substrate for inhibition testing: phthalic acid; Reference Concentration: 2.76 mg/L; ThOD Reference Substance: 1.44 mg ThOD/mg reference substance
STATISTICAL METHODS:
The amount of oxygen consumed within the test system is compared ‘to the ThOD of the test item and the percent (%) degradation is calculated as follows:
((mg/L O2 uptake at time = t (in days)) / (mg/L Test Item (as ThOd)) x 100% = % degradation over time
Using this method, the extent and rate of biodegradability is determined based on the cumulative uptake of molecular oxygen. Test items exhibiting a biochemical oxygen demand (BOD) of > 60% of the total oxygen demand (based on ThOD) in a 28-day period are considered to be readily biodegradable (OECD, 1992). Additionally, the test item must achieve 60% biodegradation within 14 days of attaining 10% biodegradation (this criterion is referred to as the "fourteen-day window”).
Reference substance
- Reference substance:
- other: Phthalic Acid, ACS, 99.5+% (Assay)
- Remarks:
- CAS # 88-99-3, Lot Number: C14K39, Chemical Name/Synonym: 1,2-Benzenedicarboxylic acid; o-dicarboxybenzene Chemical Formula: C6H4(COOH)2
Results and discussion
- Test performance:
- A reference substance testing was conducted in parallel with the definitive test to demonstrate consistency in laboratory test performance that might be affected by such factors as inoculum quality and performance of technical staff. The reference substance used in this study was phthalic acid.
REFERENCE TEST: Passed (i.e., ≥60% degradation in 14 days).
INHIBITION TEST: 7.17 mg/l test substance, 2.76mg/l reference substance; Inhibition not observed (i.e., >25% degradation)
The reference substance test passed the criteria for acceptability of the results.
% Degradationopen allclose all
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 0 d
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 7 d
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- -1.2
- Sampling time:
- 14 d
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 21 d
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 1.2
- Sampling time:
- 28 d
- Details on results:
- The test item is not readily biodegradable (i.e., < 60% degradation in 28 days).
Any other information on results incl. tables
REFERENCE SUBSTANCE AND INHIBITION DATA
Reference: Phthalic Acid
Day | 0 | 7 |
14 | 21 | 28 |
Reference, % degradation at time = x |
0.0 |
71.7 |
88.1 |
88.1 |
94.4 |
Inhibition, % degradation at time = x |
0.0 |
>25 |
>25 |
>25 |
>25 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The test item is not readily biodegradable (i.e., <60% degradation in 28 days). The test item is not inhibitory on the degradation of phthalic acid.
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