Registration Dossier
Registration Dossier
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EC number: 211-923-5 | CAS number: 712-50-5
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 13-Oct-2009 to 22-Jan-2010
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: state of the art experiment that is limited to distinguish corrosive from non-corrosive substances
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guideline
- Qualifier:
- no guideline available
- Guideline:
- other: recommended initial/alternative test
- Deviations:
- not applicable
- Principles of method if other than guideline:
- The potential of a test substance to cause serious damage to the eyes/mucous membranes is assessed by a single topical applicationof the undiluted and diluted test substance to the chorioallantoic membrane (CAM) of fertilized and incubated hen eggs.
Three eggs per test-substance concentration were observed up to 90 (undiluted test
substance) or 160 seconds (10% solution in olive oil) and the occurrence of vascular injury or
intravascular coagulation in response to the test substance was recorded. - GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Cyclohexylphenylketone
- IUPAC Name:
- Cyclohexylphenylketone
- Details on test material:
- cyclohexylphenylketone
Constituent 1
Test animals / tissue source
- Species:
- other: hen eggs
- Details on test animals or tissues and environmental conditions:
- fresh fertilized hen eggs produced under SPF conditions
Test system
- Vehicle:
- other: olive oil
- Amount / concentration applied:
- 25 μL bulk volume (about 26 mg) of the undiluted test substance and
0.3 mL of a 10% test-substance solution in olive oil - Duration of treatment / exposure:
- 90 seconds (undiluted test material) or 160 seconds (diluted test material)
- Observation period (in vivo):
- not applicable
- Number of animals or in vitro replicates:
- 3 eggs per concentration
Results and discussion
In vivo
Results
- Irritation parameter:
- other: HET-CAM
- Basis:
- other: chorioallantoic membrane, hen egg
- Time point:
- other: 90 seconds (undiluted test material) or 160 seconds (diluted test material) after application
- Score:
- 0
- Max. score:
- 3
- Reversibility:
- other: no findings
- Irritant / corrosive response data:
- The chorioallantoic membrane of the eggs treated with the undiluted test substance or with
the 10% test-substance solution in olive oil did not show any irritation effects. - Other effects:
- none
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
