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EC number: 613-855-5 | CAS number: 65928-65-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Jan - Mar 2003
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- Principles of method if other than guideline:
- not relevant
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Vehicle:
- no
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- <= 1 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- <= 1 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Validity criteria fulfilled:
- yes
- Conclusions:
- 5-Dehydrocyanomethylketon had a concentration-depended inhibitory effect on the growth of Desmodesmus subspicatus. The EbC50 (biomass) and the ErC50 (growth rate) was below 1 mg/L. The highest concentration without effect could not be determined, since it was below the lowest
concentration tested. Consequently, ZK 37877 was considered to be very toxic to the green algae Desmodesmus subspicatus, since the EC50
was < 1 mg/L. - Executive summary:
The purpose of this study was to determine the effects of the test compound 5 -Dehydrocyanomethylketon (ZK 37877) on the growth of the green algae Desmodesmus subspicatus. ZK 37877 is an intermediate of the synthesis of Dienogest. The study was conducted in agreement with the test guideline OECD no. 201.
The test substance was incubated in an aqueous solution including nutrients with an algae population of Desmodesmus subspicatus for a test duration of approximately 72 hours in an electronically controlled dosing and incubation apparatus. The nutrient solution was made up of mainly nitrate, phosphates and some trace elements. For the preparation of the test solutions a suspension with a nominal loading of 125 mg/L ZK 37877 was suspended in water, ultrasonified for approximately 30 min and constantly stirred for approximately 24 hours. This suspension was filtered through a glassfibre filter. The resulting solution was used for the preparation of the test solutions. The highest concentration was obtained from a I: 1.25 dilution by adding nutrient solution and inoculum, the further concentrations were obtained by dilutions of 1:5, 1:10, 1:25, 1:50 and 1:100 with demineralized water, nutrient solution and inoculum. Additionally, a control solution was prepared with demineralized water without test material. The organic carbon concentration of the stock solution was analyzed with a TOC analyzer in a sample taken at the start of the incubation. The substance concentration was calculated on the basis of the molecular fonnula. It was 24.4 mg/L. The further test concentrations were extrapolated from the result ofthe TOC-analysis.
As a parameter for the growth of the algae population, the fluorescence of the algal cells was measured with a fluorescence-photometer. The increase of biomass and the growth rate were calculated on the basis of the fluorescence. The calculated biomass and growth rate of each concentration were compared to those of the controls, and the inhibition was calculated.
The biomass and growth rate was inhibited at all test concentrations. This was considered to be a compound related effect.
Reference
Table 1: Inhibition of biomass and the growth rate (%) of Desmodesmus subspicatus after 72 hours exposure to ZK37877
Inhibition in percent | |||
Dilution of ZK37877 | Concentration of ZK 37877 (mg/L) | Biornass (integral) | Growth rate |
0 | 0,0 (control) | 0 | 0 |
1:100 | 0,24 | 80,7 | 64 |
1:50 | 0,49 | 82,4 | 71,1 |
1:25 | 0,97 | 84,6 | 77,3 |
1:10 | 2,44 | 88,9 | 92,9 |
1:5 | 4,87 | 86,1 | 83,6 |
1: 1,25 | 19,5 | 93,8 | 101,4 |
Description of key information
5-Dehydrocyanomethylketon had a concentration-depended inhibitory effect on the growth of Desmodesmus subspicatus. The EbC50 (biomass) and the ErC50 (growth rate) was below 1 mg/L. The highest concentration without effect could not be determined, since it was below the lowest
concentration tested. Consequently, ZK 37877 was considered to be very toxic to the green algae Desmodesmus subspicatus, since the EC50
was < 1 mg/L.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 1 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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